Microarray Design for Measuring Contraction of Aligned Isolated Myotubes
Sora Jinnai, Shigehiro Hashimoto
Proceedings of the 27th World Multi-Conference on Systemics, Cybernetics and Informatics: WMSCI 2023, pp. 36-39 (2023); https://doi.org/10.54808/WMSCI2023.01.36
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The 27th World Multi-Conference on Systemics, Cybernetics and Informatics: WMSCI 2023
Virtual Conference September 12 - 15, 2023 Proceedings of WMSCI 2023 ISSN: 2771-0947 (Print) ISBN (Volume): 978-1-950492-73-2 (Print) |
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Abstract
Measurement of single-myotube contraction is essential for the evaluation of engineered tissues. A microarray-associated scaffold plane was designed to evaluate the contraction of multiple myotubes simultaneously in vitro. Considering the dimensions of a single myotube, a microcylinder array with a diameter of 10 μm and an interval of 20 μm was created on the scaffold plane by photolithography. Cells will be cultured on columnar projections of 20 μm in height or on the flat membrane surface with micro-projections on the back. To culture each myotube in isolation, a layer of water-soluble gel is used. Concave spaces are prepared at intervals on the gel surface, and myoblasts will be seeded in the concave spaces. After the myoblasts differentiate and fuse into myotubes, the gel layer is dissolved and removed. Cyclic electrical pulses will be applied through electrodes immersed in the medium. The repetitive contractile motion of myotubes will be measured by the synchronized deformation of the micro-columns or the movement of back projections accompanying the deformation of membrane. Contraction motion of multiple myotubes can be measured simultaneously. By comparing the motion in membranes with protrusions on the back surface, the effect of the shape of the microcylinder can be evaluated.
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