Effect of Periodical Wall Shear Stress on Cultured Cell: Design of Couette Flow Device
Yuuki Takabe, Shigehiro Hashimoto
Proceedings of the 15th International Multi-Conference on Complexity, Informatics and Cybernetics: IMCIC 2024, pp. 20-26 (2024); https://doi.org/10.54808/IMCIC2024.01.20
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The 15th International Multi-Conference on Complexity, Informatics and Cybernetics: IMCIC 2024
Virtual Conference March 26 - 29, 2024 Proceedings of IMCIC 2024 ISSN: 2771-5914 (Print) ISBN (Volume): 978-1-950492-78-7 (Print) |
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Abstract
Endothelial cells are oriented in the direction of blood flow on the inner wall of blood vessels. Endothelial cells are exposed to pulsatile flow and are subjected to periodic wall shear stress. In this study, a device was designed to apply periodically fluctuating shear stress to cells. A Couette-type flow was generated in the culture medium sandwiched between a rotating disk and a stationary culture plate. By periodically varying the rotational speed of the disk in a rectangular wave pattern, periodically varying shear stress was applied to the culture wall surface. The device was placed in an incubator so that cells in culture could be continuously observed under a microscope while being exposed to a cyclic shear stress field. The flow of the culture medium was measured by tracking the floating cells as tracers. It was confirmed that the flow fluctuated in a rectangular wave pattern following the rotation of the disk. This device allows observation of the behavior of cultured cells under periodic wall shear stress. Research on cellular responses to mechanical stimulation is expected to be applied to regenerative medicine through tissue formation.
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