Published online Sep 30, 2010.
https://doi.org/10.5395/JKACD.2010.35.5.313
Real-time measurement of dentinal fluid flow during desensitizing agent application
Abstract
Objectives
The aim of this study was to examine changes in the dentinal fluid flow (DFF) during desensitizing agent application and to compare permeability after application among the agents.
Materials and Methods
A Class 5 cavity was prepared to exposure cervical dentin on an extracted human premolar which was connected to a sub-nanoliter fluid flow measuring device (NFMD) under 20 cm water pressure. DFF was measured from before application of desensitizing agent (Seal&Protect, SP; SuperSeal, SS; BisBlock, BB; Gluma desensitizer, GL; Bi-Fluoride 12, BF) through application procedure to 5 min after application.
Results
DFF rate after each desensitizing agent application was significantly reduced when compared to initial DFF rate before application (p < 0.05). SP showed a greater reduction in DFF rate than GL and BF did (p < 0.05). SS and BB showed a greater reduction in DFF rate than BF did (p < 0.05).
Conclusions
Characteristic DFF aspect of each desensitizing agent was shown in NFMD during the application procedure.
Figure 1
(a) Schematic diagram of the sub-nanoliter scaled dentinal fluid flow measurement system connected to a specimen. (b) Specimen preparation.
Figure 2
A representative curve of consecutive dentinal fluid flow (DFF) during Bi-Fluoride 12 application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; d, air dry.
Figure 3
A representative curve of consecutive DFF during Gluma desensitizer application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; d, air dry; r, rinse.
Figure 4
A representative curve of consecutive DFF during BisBlock application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; r, rinse.
Figure 5
A representative curve of consecutive DFF during SuperSeal application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; ga, gentle air dry.
Figure 6
A representative curve of consecutive DFF during Seal&Protect application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; LC, light-curing.
Figure 7
Reduction in dentinal fluid flow of desensitizing agents (%). Desensitizing agents under same bar did not show statistically significant difference.
Table 1
Components and application procedures of desensitizing agents used in this study
References
-
Brännström M, Lindén LA, Aström A. The hydrodynamics of the dental tubule and of pulp fluid. A discussion of its significance in relation to dentinal sensitivity. Caries Res 1967;1:310–317.
-
-
Pashley DH. Potential treatment modalities for dentine hypersensitivity: in-office products. In: Addy M, Embrey G, Edgar WM, Orchardson R, editors. Tooth wear and sensitivity: clinical advances in restorative dentistry. London: Martis Dunitz; 2000. pp. 351-365.
-
-
Vieira AH, Santiago SL. Management of dentinal hypersensitivity. Gen Dent 2009;57:120–126.
-
-
Lee IB, Kim MH, Kim SY, Chang J, Cho BH, Son HH, Back SH. Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid. J Korean Acad Conserv Dent 2008;33:141–147.
-
-
Kim SY, Cho BH, Baek SH, Lim BS, Lee IB. Real-time measurement of dentinal tubular fluid flow during and after amalgam and composite restorations. J Korean Acad Conserv Dent 2009;34:467–476.
-
-
Itthagarun A, Tay FR, Pashley DH, Wefel JS, Garclía-Godoy F, Wei SH. Single-step, self-etch adhesives behave as permeable membranes after polymerization. Part III. Evidence from fluid conductance and artificial caries inhibition. Am J Dent 2004;17:394–400.
-
-
Camps J, About I, Van Meerbeek B, Franquin JC. Efficiency and cytotoxicity of resin-based desensitizing agents. Am J Dent 2002;15:300–304.
-
-
Paes Leme AF, dos Santos JC, Giannini M, Wada RS. Occlusion of dentin tubules by desensitizing agents. Am J Dent 2004;17:368–372.
-
-
Thrash WJ, Jones DL, Dodds WJ. Effect of a fluoride solution on dentinal hypersensitivity. Am J Dent 1992;5:299–302.
-
-
Kakaboura A, Rahiotis C, Thomaidis S, Doukoudakis S. Clinical effectiveness of two agents on the treatment of tooth cervical hypersensitivity. Am J Dent 2005;18:291–295.
-
-
Davidson DF, Suzuki M. The Gluma bonding system: a clinical evaluation of its various components for the treatment of hypersensitive root dentin. J Can Dent Assoc 1997;63:38–41.
-
-
Ishihata H, Kanehira M, Nagai T, Finger WJ, Shimauchi H, Komatsu M. Effect of desensitizing agents on dentin permeability. Am J Dent 2009;22:143–146.
-