Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
권호 표지
DOI QR코드

DOI QR Code

Development and Characterization of Horse Bone-derived Natural Calcium Phosphate Powders

  • Jang, Kyoung-Je (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Cho, Woo Jae (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Seonwoo, Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Jangho (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Lim, Ki Taek (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Chung, Pill-Hoon (Department of Oral and Maxillofacial Surgery and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Chung, Jong Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University)
  • Received : 2014.05.16
  • Accepted : 2014.05.27
  • Published : 2014.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: This study was to develop an effective process for fabricating biocompatible calcium phosphate powders (CPPs) using horse bones, and to investigate the characteristics of them. Methods: The characteristics of horse bone powders (HBPs) were investigated according to the different osseous tissue types (compact bone and cancellous bone), bone types (spine and tibia), pretreatment methods (cold water, $H_2O_2$, and hot water), sintering time (4, 8 and 12h), and sintering temperature (600, 900, 1100 and $1300^{\circ}C$). In addition, the grinding methods were compared based on the wet grinding (ball mill) and dry grinding (blade grinder) method to make it as powders. Finally, their cytotoxicity and cell viability were checked. Results: Regardless of the types of osseous tissues and bones, HBPs were well fabricated as biocompatible CPPs. It was also found that the pretreatment methods did not influence on the resultants, showing well-fabricated HBPs. Considering the processing time, the hot water method was the most suitable compared to other pretreatment methods. Further, 12h-sintering time was sufficient to remove residual organic compounds. The sintering temperatures greatly affected the properties of bone powders fabricated. The x-ray diffraction (XRD) peak of horse bone sintered at $600^{\circ}C$ was most closed to that of hydroxyapatite (HA). Our bioactivity study demonstrated that the HBPs fabricated by sintering horse bones at $1300^{\circ}C$ showed the best performance in terms of cell viability whereas the HBPs $1100^{\circ}C$ showed the cytotoxicity. Conclusions: Using various types of horse bone tissues, biocompatible CPPs were successfully developed. We conclude that the HBPs may have a great potential as biomaterials for various biological applications including bone tissue engineering.

Keywords

References

  1. Bahrololoom, M. E., Javidi, M., Javadpour, S and J. Ma. 2009. Characterisation of natural hydroxyapatite extracted from bovine cortical bone ash. Journal of Ceramic Processing Research, 10(2):129-138.
  2. Baik, S. J. 2011. Development of Fast-Hardening Calcium Phosphate Cement Using Sintered Animal Bone Powders and Chitosan Solution for Bone Tissue Engineering. (The Degree of Master A Thesis for the Degree of Master), Seoul National University, Seoul.
  3. Barakat, N. A. M., Khalil, K. A., Sheikh, F. A., Omran, A. M., Gaihre, B., Khil, S. M and H. Y. Kim. 2008. Physiochemical characterizations of hydroxyapatite extracted from bovine bones by three different methods: Extraction of biologically desirable HAp. Materials Science & Engineering C-Biomimetic and Supramolecular Systems, 28(8):1381-1387. doi: DOI 10.1016/j.msec.2008.03.003.
  4. Betz, R. R. 2002. Limitations of autograft and allograft: new synthetic solutions. Orthopedics, 25(5 Suppl), s561-570.
  5. Cai, Y. R., Liu, Y. K., Yan, W. Q., Hu, Q. H., Tao, J. H., Zhang, M and R. K. Tang. 2007. Role of hydroxyapatite nanoparticle size in bone cell proliferation. Journal of Materials Chemistry, 17(36):3780-3787. doi: Doi 10.1039/B705129h.
  6. Danilchenko, S., Koropov, A., Protsenko, I. Y., Sulkio-Cleff, B and L. Sukhodub. 2006. Thermal behavior of biogenic apatite crystals in bone: An X-ray diffraction study. Crystal Research and Technology, 41(3):268-275. https://doi.org/10.1002/crat.200510572
  7. Fernandez-Moran, H and A. Engstrom. 1957. Electron microscopy and x-ray diffraction of bone. Biochim Biophys Acta, 23(2):260-264. https://doi.org/10.1016/0006-3002(57)90327-X
  8. Guizzardi, S., Montanari, C., Migliaccio, S., Strocchi, R., Solmi, R., Martini, D and A. Ruggeri. 2000. Qualitative assessment of natural apatite in vitro and in vivo. J Biomed Mater Res, 53(3):227-234. https://doi.org/10.1002/(SICI)1097-4636(2000)53:3<227::AID-JBM7>3.0.CO;2-E
  9. Haberko, K., Bucko, M. M., Brzezinska-Miecznik, J., Haberko, M., Mozgawa, W., Panz, T and J. Zarebski. 2006. Natural hydroxyapatite-its behaviour during heat treatment. Journal of the European Ceramic Society, 26(4):537-542. https://doi.org/10.1016/j.jeurceramsoc.2005.07.033
  10. Johnell, O and J. A. Kanis. 2006. An estimate of the worldwide prevalence and disability associated with osteoporotic fractures. Osteoporos Int, 17(12):1726-1733. doi: 10.1007/s00198-006-0172-4.
  11. Kim, H., Rey, C and M. J. Glimcher. 1996. X-ray diffraction, electron microscopy, and Fourier transform infrared spectroscopy of apatite crystals isolated from chicken and bovine calcified cartilage. Calcif Tissue Int, 59(1): 58-63. https://doi.org/10.1007/s002239900086
  12. Lafon, J. P., Champion, E., Bernache-Assollant, D., Gibert, R and A. M. Danna. 2003. Termal decomposition of carbonated calcium phosphate apatites. Journal of Thermal Analysis and Calorimetry, 72(3):1127-1134. https://doi.org/10.1023/A:1025036214044
  13. Laird, D. F. 2010. Reinforced Sintered Cancellous Bovine Bone as a Potential Bone Replacement Material. University of Waikato.
  14. Langer, R and J. P. Vacanti. 1993. Tissue engineering. Science, 260(5110):920-926. https://doi.org/10.1126/science.8493529
  15. Laquerriere, P., Kilian, L., Bouchot, A., Jallot, E., Grandjean, A., Guenounou, M and P. Bonhomme. 2001. Effect of hydroxyapatite sintering temperature on intracellular ionic concentrations of monocytes: A TEM-cryo-x-ray microanalysis study. Journal of biomedical materials research, 58(3):238-246. https://doi.org/10.1002/1097-4636(2001)58:3<238::AID-JBM1012>3.0.CO;2-I
  16. Mezahi, F. Z., Oudadesse, H., Harabi, A., Lucas-Girot, A., Le Gal, Y., Chaair, H and G. Cathelineau. 2009. Dissolution kinetic and structural behaviour of natural hydroxyapatite vs. thermal treatment. Journal of Thermal Analysis and Calorimetry, 95(1):21-29. doi: DOI 10.1007/s10973-008-9065-4.
  17. Mondal, S., Mondal, B., Dey, A and S. S. Mukhopadhyay. 2012. Studies on Processing and Characterization of Hydroxyapatite Biomaterials from Different Bio Wastes. Journal of Minerals & Materials Characterization, 55-67.
  18. Moore, W. R., Graves, S. E and G. I. Bain. 2001. Synthetic bone graft substitutes. ANZ J Surg, 71(6):354-361. https://doi.org/10.1046/j.1440-1622.2001.02128.x
  19. Ooi, C. Y., Hamdi, M and S. Ramesh. 2007. Properties of hydroxyapatite produced by annealing of bovine bone. Ceramics international, 33(7):1171-1177. doi: DOI 10.1016/j.ceramint.2006.04.001.
  20. Ozawa, M and S. Suzuki. 2002. Microstructural Development of Natural Hydroxyapatite Originated from Fish-Bone Waste through Heat Treatment. Journal of the American Ceramic Society, 85(5):1315-1317.
  21. Panda, N. N., Pramanik, K and L. B. Sukla. 2013. Extraction and characterization of biocompatible hydroxyapatite from fresh water fish scales for tissue engineering scaffold. Bioprocess Biosyst Eng, 1-8. doi: 10.1007/s00449-013-1009-0.
  22. Quarles, L. D., Yohay, D. A., Lever, L. W., Caton, R and R. J. Wenstrup. 1992. Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: An in vitro model of osteoblast development. Journal of Bone and Mineral Research, 7(6):683-692.
  23. Rodrigues, C., Serricella, P., Linhares, A., Guerdes, R., Borojevic, R., Rossi, M and M. Farina. 2003. Characterization of a bovine collagen-hydroxyapatite composite scaffold for bone tissue engineering. Biomaterials, 24(27):4987-4997. https://doi.org/10.1016/S0142-9612(03)00410-1
  24. Shi, Z., Huang, X., Cai, Y., Tang, R and D. Yang. 2009. Size effect of hydroxyapatite nanoparticles on proliferation and apoptosis of osteoblast-like cells. Acta Biomater, 5(1):338-345. doi: 10.1016/j.actbio.2008.07.023.
  25. Skalak, R and C. Fox. 1988. Tissue Engineering, Alan R. Liss. Inc., New York.
  26. Sobczak, A., Kowalski, Z and Wzorek, Z. 2009. Preparation of hydroxyapatite from animal bones. Acta Bioeng Biomech, 11(4):23-28.
  27. Taniguchi, Y., Tamaki, T., Oura, H., Hashizume, H and A. Minamide. 1999. Sintered bone implantation for the treatment of benign bone tumours in the hand. J Hand Surg Br, 24(1):109-112. https://doi.org/10.1016/S0266-7681(99)90055-4
  28. Tsai, W. C., Liao, C. J., Wu, C. T., Liu, C. Y., Lin, S. C., Young, T. H and H. C. Liu. 2010. Clinical result of sintered bovine hydroxyapatite bone substitute: analysis of the interface reaction between tissue and bone substitute. Journal of Orthopaedic Science, 15(2):223-232. doi: DOI 10.1007/s00776-009-1441-9.
  29. Ueno, Y., Sasaki, S., Shima, Y., Ueyoshi, A., Harada, M., Terao, K and T. Akiyama. 1983. Studies of sintered bone as a bone substitute. Orthop Ceramic Implants, 3:11-16.
  30. Wang, C. Y., Duan, Y. R., Markovic, B., Barbara, J., Howlett, C. R., Zhang, X. D and H. Zreiqat. 2004. Proliferation and bone-related gene expression of osteoblasts grown on hydroxyapatite ceramics sintered at different temperature. Biomaterials, 25(15):2949-2956. doi: DOI 10.1016/j.biomaterials.2003.09.088.
  31. Wang, J., de Boer, J and K. de Groot. 2008. Proliferation and differentiation of MC3T3-E1 cells on calcium phosphate/chitosan coatings. J Dent Res, 87(7):650-654. https://doi.org/10.1177/154405910808700713
  32. Wang, X. Y., Zuo, Y., Huang, D., Hou, X. D and Y. B. Li. 2010. Comparative study on inorganic composition and crystallographic properties of cortical and cancellous bone. Biomedical and Environmental Sciences, 23(6): 473-480. https://doi.org/10.1016/S0895-3988(11)60010-X

Cited by

  1. Development and characterization of fast-hardening composite cements composed of natural ceramics originated from horse bones and chitosan solution vol.11, pp.5, 2014, https://doi.org/10.1007/s13770-014-0036-5