ISIS Neutron and Muon Source Data Journal

Satisfying the need for contrast in antimicrobial research Completion

Abstract: Gram-negative bacteria, which cause a variety of diseases including meningitis, plague, sepsis and food poisoning, are becoming increasingly antibiotic resistant. One reason is their robust outer membrane (OM) which can resist antibiotic penetration. We have been combining our recently developed OM model with neutron science to measure the penetration of an important antibiotic, polymyxin (PMB), into the OM. These have been supported by in vivo data on live bacteria. We need to measure where the antibiotic resides in the OM model and this proposal seeks to follow up experiments on a fully deuterated OM model using deuterated -LPS produced in our own laboratory which were cut short by a beam failure. We were able to demonstrate that the d-LPS worked and were starting to see the antibiotic insertion when the beam failed we thus need replacement time to complete this important experiment.

Public release date: 21 September 2021

Principal Investigator: Professor Jeremy Lakey
Local Contact: Dr Nina-Juliane Steinke
Experimenter: Dr Luke Clifton
Experimenter: Dr Nico Paracini

DOI: 10.5286/ISIS.E.98213981

Parent DOI: 10.5286/ISIS.E.RB1900032

ISIS Experiment Number: RB1900032

Part Number: 1

Date of Experiment: 19 September 2018

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey et al; (2018): Satisfying the need for contrast in antimicrobial research Completion, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.98213981