ISIS Neutron and Muon Source Data Journal

Effect of serum on the structure of gene delivery vehicles

Abstract: To date complexes prepared from vesicles composed of dichain, cationic lipids (L) with negatively charged DNA (D), known as LD complexes have emerged as one of the major non-viral gene delivery vehicles. Despite the wide spread interest in LD complexes as transfection agents, very little is understood as to their mechanism of action. It is widely believed that understanding their mechanism of action and the barriers to transfection they encounter upon administration will enable the development of improved gene delivery agents. Among the barriers to transfection is serum. The stability of gene delivery vehicles in the presence of serum can be regarded as a first approximation to gain an insight into the behaviour of such lipid-based carriers in the body after injection. This proposal aims to study the effect of serum on the detailed nanostructure of gene delivery vehicles using SANS.

Public release date: 25 March 2015

Principal Investigator: Professor Jayne Lawrence
Experimenter: Dr Laila Kudsiova
Experimenter: Ms Fabrizia Foglia
Experimenter: Dr Helen Hailes
Experimenter: Dr Alethea Tabor
Experimenter: Dr Fabrizia Foglia
Experimenter: Dr Katharina Welser

DOI: 10.5286/ISIS.E.24088666

ISIS Experiment Number: RB1210318

Part Number: 1 - LOQ

Date of Experiment: 21 March 2012

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jayne Lawrence et al; (2012): Effect of serum on the structure of gene delivery vehicles, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.24088666