ISIS Neutron and Muon Source Data Journal

This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.


Using Magnetic Contrasts to Establish the Mechanism and Kinetics of Lipid Flip-Flop in Floating Phosphatidylserine Bilayers

Abstract: In order to maintain their asymmetric lipid distribution, cellular membranes have a number of means to translocate lipid molecules across the bilayer. On top of the well-studied active flipping processes carried out by the flippases & floppases, there are also passive transfer mechanisms which facilitate the lipid migration even in protein-free bilayers. This process, is considered to be the significant natural process that cells must overcome in order to sustain 'normal' cell asymmetry. Despite this, the mechanism by which this occurs in a protein independent manner is poorly understood and warrants investigation.We aim to build selectively deuterated floating lipid bilayers on silicon with a buried iron layer, this will allow the system to be studied at two contrasts using polarised neutrons without the need to exchange the solvent, allowing us to observe the rate of lipid exchange.

Public release date: 07 September 2015

Principal Investigator: Dr Stephen Roser
Experimenter: Dr Rob Barker
Experimenter: Dr Arwel Hughes

DOI: 10.5286/ISIS.E.24079736

ISIS Experiment Number: RB1010280

Part Number: 1 - POLREF

Date of Experiment: 23 April 2010

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Stephen Roser et al; (2010): Using Magnetic Contrasts to Establish the Mechanism and Kinetics of Lipid Flip-Flop in Floating Phosphatidylserine Bilayers, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.24079736



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