ISIS Neutron and Muon Source Data Journal

Self-assembly of protein polymer conjugates: Effect of copolymer structure

Abstract: Conjugation of therapeutic proteins to polymers is well established but most methods result in multi-site and multiple conjugation or crosslinking resulting in lack of orientation and loss of activity. Using an enzyme mediated conjugation method we have prepared well-defined protein-polymer conjugates using a model protein, green fluorescent protein (GFP) with diblock copolymers based on a several hydrophilic and hydrophobic biocompatible polymers. In this proposal we wish to determine the effects of block copolymer structure on the properties of aggregates formed by these polymers & conjugates with GFP in solution, to develop an understanding of the phase behaviour of these species. We will also study GFP-poly(N-isopropylacrylamide) a polymer which becomes less soluble as the temperature increases to observe changes in aggregation state based on polymer hydrophobicity.

Public release date: 21 September 2016

Principal Investigator: Professor Karen Edler
Experimenter: Ms Duygu Celebi
Experimenter: Dr Gavin Hazell
Experimenter: Dr Susanna Piluso
Experimenter: Mr Yun Jin
Experimenter: Dr Gabriel Cavalli
Experimenter: Dr Amani El Fagui

DOI: 10.5286/ISIS.E.42593636

ISIS Experiment Number: RB1320432

Part Number: 1

Date of Experiment: 17 September 2013

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2013): Self-assembly of protein polymer conjugates: Effect of copolymer structure, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.42593636