ISIS Neutron and Muon Source Data Journal

The Interaction of wild-type and mutant Puroindoline B with Model Bacterial Membranes

Abstract: Bacterial and fungal infections of cereal crops pose considerable risk to food security. Pathogenic resistance to conventional fungicides and pesticides is becoming widespread and, therefore, research into naturally occurring alternative antimicrobial agents is timely. Puroindolines are found in wheat endosperm and play a role in seed defence. Puroindoline B interacts with lipids via an unstructured arginine- and tryptophan-rich loop and we have shown that naturally occurring point mutations in this region lead to differences in the mode of lipid binding. We aim to use neutron reflectivity to determine the interfacial layer structure of the peptide binding to model bacterial bilayers. The findings will support our NMR and EPR studies which have highlighted differences in lipid head group dynamics as a result of binding of wild type and mutant puroindoline B.

Public release date: 12 February 2017

Principal Investigator: Professor Rebecca Green
Experimenter: Professor Anthony Watts
Experimenter: Dr Peter Judge
Experimenter: Mr Mike Sanders
Experimenter: Mr Daniel Yin
Experimenter: Mr Marc-Philipp Pfeil
Experimenter: Professor Richard Frazier
Experimenter: Dr Luke Clifton

DOI: 10.5286/ISIS.E.42583370

ISIS Experiment Number: RB1320341

Part Number: 1

Date of Experiment: 18 December 2013

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Rebecca Green et al; (2013): The Interaction of wild-type and mutant Puroindoline B with Model Bacterial Membranes, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.42583370