ISIS Neutron and Muon Source Data Journal

Probing the internal structure of gene delivery vehicles using contrast variation SANS

Abstract: Gene delivery vehicles, known as LPD complexes, prepared from mixing cationic lipid vesicles (L) with cationic peptide (P) and DNA (D) have proven to be very effective at delivering DNA into the nucleus of cells to obtain expresion. Although a considerable amout of work has gone into developing new cationic lipids that improve the level of DNA expression, far less work has investigated the effect of peptide on transfection. The present study aims to determine the structure of LPD complexes as a function of peptide structure. Studies have shown that peptide structure has a significant affect on transfection while electron microscopy studies have shown that as transfection decreases the amount of order seen in the core in the LPD complex increases. Contrast variation SANS will be used to probe the detailed structure of the LPD complex to understand the transfection results.

Public release date: 04 April 2015

Principal Investigator: Professor Jayne Lawrence
Experimenter: Dr Dave Barlow
Experimenter: Dr Laila Kudsiova
Experimenter: Ms Fabrizia Foglia
Experimenter: Dr Helen Hailes
Experimenter: Dr Alethea Tabor
Experimenter: Dr Fabrizia Foglia
Experimenter: Dr Katharina Welser
Experimenter: Ms Lili Cui
Experimenter: Miss Louise Collins
Experimenter: Dr Prashant Balani

DOI: 10.5286/ISIS.E.24088578

ISIS Experiment Number: RB1120399

Part Number: 1 - SANS2D

Date of Experiment: 25 March 2012

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jayne Lawrence et al; (2012): Probing the internal structure of gene delivery vehicles using contrast variation SANS, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.24088578