ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
In situ study of destruction of a denatured surface protein by protease (Savinase)
Abstract: New detergents will increasingly rely on sustainable products and the use of enzymes will become ever more important. However, the process of destruction of stains (often proteinaceous) by enzymes and the removal of residues from the surface is a complex process. We propose to use proteins as model stains and follow directly their destruction by a protease by neutron reflection with and without added surfactant. This should (a) give insights into the molecular process of cleaning and (b) give fundamental information about interfacial enzyme kinetics. We will use the well characterized enzyme, Savinase, and supported model layers of BSA denatured at a hydrophobic surface (OTS on silicon). Previous studies at the hydrophilic surface have shown that surfactant swells the BSA layer which, combined with the already denatured surface, may assist access of the layer to the attacking enzyme.
Public release date: 09 June 2012
Principal Investigator: Dr Diana Taylor
Experimenter: Professor Jeff Penfold
Experimenter: Dr Bob Thomas
DOI: 10.5286/ISIS.E.24068757
ISIS Experiment Number: RB910343
Part Number: 1 - SURF
Date of Experiment: 06 June 2009
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Diana Taylor et al; (2009): In situ study of destruction of a denatured surface protein by protease (Savinase), STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.24068757
