Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers
Creators
- 1. Department of Karyosystematics, Zoological Institute, Russian Academy of Sciences, Universitetskaya nab. 1, 199034 Saint-Petersburg, Russia
- 2. Department of Entomology, Biological Faculty, Lomonosov Moscow State University, Leninskie Gory, GSP-1, korp. 12, 119991 Moscow, Russia; Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia
- 3. Department of Ecology, Altai State University, Lenina Pr. 61, 656049 Barnaul, Russia; Institute of Biology, Tomsk State University, Lenina Pr. 36, 634050 Tomsk, Russia
- 4. Centro de Investigaciones en Optica, Lomas del Bosque 115, 37150 Leon, Mexico
Description
In this report, we analyze a wild-caught Colias specimen from Kyrgyzstan with unusual wing pattern, which was provisionally identified as a natural hybrid between Colias christophi helialaica Schulte, 1988 and Colias cocandica cocandica Erschoff, 1874 due to its intermediate phenotype. Using one mitochondrial (COI barcode) and three nuclear (Ca-ATPase, H3 and CAD gene fragments) markers, we confirm its hybrid origin and demonstrate that this specimen is a hybrid between a female of Colias christophi helialaica and a male of Colias cocandica cocandica. Our study shows that an analysis of mitochondrial and nuclear molecular markers can successfully discriminate natural hybrids in such a taxonomically challenging group of butterflies as the genus Colias.
Notes
Files
Shapoval et al_final.pdf
Files
(17.9 MB)
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