Mapped data: Transcription factor stoichiometry, motif affinity and syntax regulate single cell chromatin dynamics during fibroblast reprogramming to pluripotency

This record contains mapped sequencing data for the paper "Transcription factor stoichiometry, motif affinity and syntax regulate single cell chromatin dynamics during fibroblast reprogramming to pluripotency" by Nair, Ameen et al. It contains single-cell RNA-seq (scRNA) and single-cell ATAC-seq (scATAC) data from a time course of human dermal fibroblasts induced with Yamanaka factors OSKM using a Sendai virus based delivery system. The scRNA and scATAC data is performed at days 0, 2, 4, 6, 8, 10, 12, 14 and the final iPSCs. The experiment was re-performed and single-nucleus multiome (ATAC+RNA) was collected on days 1 and 2. 

The data is as follows:

scATAC: We used Chromap (commit https://github.com/haowenz/chromap/tree/6e97125b9, https://doi.org/10.1038/s41467-021-26865-w) to perform barcode correction, alignment and filtering for each of our samples. The corresponding fragment files (tab separated file containing mapped fragments with columns: chr, start, end, barcode, number of reads) and their tabix indices are available for each sample.

scRNA: We used cellranger v6.0.2 for read mapping and quantification to obtain the counts matrix. We used the GRCh38 2020-A reference. For each sample, the raw and filtered counts matrices are provided. E.g. `D0/raw_feature_bc_matrix.h5` contains an HDF5 object containing gene counts for each barcode and associated metadata for the Day 0 sample. Similarly, the files in `D0/raw_feature_bc_matrix/` contain the same gene x barcode matrix, with the counts matrix in Matrix Market format (`matrix.mtx.gz`), and gene (`features.tsv.gz`) and barcode names (`barcodes.tsv.gz`). 

multiome: The ATAC and RNA components are separately processed using the same tools as mentioned above for scATAC and scRNA. Outputs are in the `snATAC` and `snRNA` subdirectories respectively. In addition, the `ATAC.RNA.bc.map.tsv` file contains a map to link snATAC barcodes to snRNA barcodes.