Ekspresowa Analiza Zagrożenia Agrofagiem: Cucurbit aphid-borne yellows virus

Cucurbit aphid-borne yellows virus (CABYV) in Poland was identified in 2018 during monitoring of zucchini crops in Wielkopolskie and Kujawsko-Pomorskie Voivodeships. Symptoms were observed on the harvested plants in the form of distorted leaves with severe chlorosis and necrosis, while the fruits were stunted and discolored. Biological, serological (DAS- and TAS-ELISA) and molecular (RT-PCR) tests were performed for the collected zucchini plant and fruit samples to identify virus species that could be the cause of the observed disease symptoms. In addition, next-generation total RNA sequencing was performed for 4 samples by an external company. Using the above methods, known viruses infecting zucchini were identified, i.e. Cucumber mosaic virus (CMV), Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV) and CABYV, which is new to Poland. What's more, mixed infections were present in the overwhelming number of samples. In 2019, during monitoring of zucchini crops in the same locations also found CABYV in infected plants and zucchini fruits.