Published December 12, 2022 | Version v1
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A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis. Figure S1. Tv-PSP1 crystal packing. Figure S2. Tv-PSP1 secondary structure and general topology.

Creators

  • 1. Instituto de Biotecnología, Universidad Nacional Autónoma de México
  • 2. Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México
  • 3. Instituto Nacional de Psiquiatría Ramón de la Fuente, Mexico

Contributors

  • 1. Facultad de Farmacia, Universidad Autónoma del Estado de Morelos
  • 2. Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México
  • 3. Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México
  • 4. Departamento de Química, Universidad Autónoma Metropolitana

Description

Figure S1. Tv-PSP1 crystal packing. Crystal packing of the hexagonal space group P63 with cell dimensions
a=81.9 Å, b=81.9 Å, c=129.3 Å, and γ=120°. A) Trimer A in Grey surface is around the threefold axis symbol. B)
Trimer D in blue steel color, this trimer is under Trimer A on the same threefold axis. The trimer D on the final
structure is not visible in a large part of the structure, only are visible the fragments in contact with monomer A,
here was built a complete Trimer from previous refinement process to illustrate the position on the crystal.
C)Trimer B in green color is around the threefold axis symbol in the symmetric object of the twofold screw axis
of the cell. D)Trimer C in orange color is around the sixfold axis symbol. Figure was made in VMD program [39]. ID PDB: 7KGC.

 

Figure S2. Tv-PSP1 secondary structure and general topology. A) Tv-PSP1 secondary structure of the
asymmetric unit monomers obtained with VMD program [39]. Marginal differences are observed on the L1 and
L7. B) General topology of the monomer A structure. Beta strands in yellow color, 3-10 helixes in blue color, alfa
helixes in magenta color, turns and coil in green color.

Notes

The first four researchers are the authors of the PDB-coded structure 7KGC: Crystal structure of a perchloric acid-soluble protein (PSP) from Trichomonas vaginalis at 1.95 A, Tv-PSP1. Adelaida Díaz-Vilchis collected diffraction data with time donated by Dr. Lilian González Segura at the 19-ID beamline of the Advanced Photon Source, Argonne National Laboratory (Argonne, IL, USA) using a Pilatus 6 M detector.

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Additional details

Related works

Is described by
Journal article: https://www.mdpi.com/2076-0817/12/1/79 (URL)
Dataset: https://www.rcsb.org/structure/7KGC (URL)

References