Human Th17 cells engage gasdermin E pores to release IL-1a upon NLRP3 inflammasome activation
Creators
- Chao, Ying-Yin1
- Puhach, Alisa1
- Frieser, David2
- Arunkumar, Mahima1
- Lehner, Laurens1
- Seeholzer, Thomas3
- Garcia-Lopez, Albert4
- Van der Wal, Marlot5
- Fibi-Smetana, Silvia6
- Dietschmann, Alex7
- Sommermann, Thomas1
- Ćiković, Tamara8
- Taher, Leila6
- Gresnigt, Mark S.7
- Vastert, Sebastiaan J.5
- Van Wijk, Femke5
- Panagiotou, Gianni4
- Krappmann, Daniel3
- Groß, Olaf8
- Zielinski, Christina E.9
- 1. Department of Infection Immunology, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany
- 2. Center for Translational Cancer Research (TranslaTUM) & Institute of Virology, Technical University of Munich, Munich, Germany
- 3. Research Unit Cellular Signal Integration, Molecular Targets and Therapeutics Center, Helmholtz Zentrum München-German Research Center for Environmental Health, Neuherberg, Germany
- 4. Department of Systems Biology and Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany
- 5. Center for Translational Immunology, University Medical Center Utrecht and Utrecht University, Utrecht, The Netherlands
- 6. Institute of Biomedical Informatics, Graz University of Technology, Graz, Austria
- 7. Adaptive Pathogenicity Strategies, Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute, Jena, Germany
- 8. Institute of Neuropathology, Medical Center & Signalling Research Centres BIOSS and CIBSS & Center for Basics in NeuroModulation (NeuroModulBasics), University of Freiburg, Faculty of Medicine, Freiburg, Germany
- 9. Department of Infection Immunology, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany; Center for Translational Cancer Research (TranslaTUM) & Institute of Virology, Technical University of Munich, Munich, Germany; Institute of Microbiology, Faculty of Biological Sciences, Friedrich Schiller University, Jena, Germany; German Center for Infection Research, Partner Site Munich, Germany; Department of Cellular Immunoregulation, Charité-Universitätsmedizin Berlin, Berlin, Germany
Description
There is evidence that innate immune responses coopt adaptive properties such as memory. Whether T cells harness innate immune signaling pathways to diversify their repertoire of effector functions remains unknown. Here, we found that human T cells expressed gasdermin E (GSDME), a membrane pore-forming molecule that has recently been shown to execute pyroptotic cell death and thus to serve as a potential cancer checkpoint. In T cells, GSDME expression was, in contrast, associated with durable viability and was repurposed for the tunneled release of the alarmin IL-1a. This property was restricted to a subset of human Th17 cells with specificity for C. albicans and was regulated by a T cell-intrinsic NLRP3 inflammasome and its engagement of a proteolytic cascade of successive caspase-8, caspase-3 and GSDME cleavage following T-cell receptor stimulation and calcium-licensed calpain maturation of the pro-IL1a form. Our results propose GSDME pore formation in T cells as a mechanism of unconventional cytokine release through harnessing of innate signaling platforms in response to adaptive stimuli. This finding diversifies the functional repertoire and mechanistic equipment of T cells with implications for anti-fungal host defense.
Notes
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