Lipid Profiling in Sprague Dawley Rats Induced with High Fat Diet (HFD)

Hypercholesterolemia is a medical condition associated with the unbalance in lipids level due to unhealthy diets and potentially leading to various heartrelated diseases if remains untreated. This study focused on lipid profiling in Sprague Dawley (SD) rats to provide more insight in future research in prognosis and treatment of cardiovascular diseases. There were 15 male Sprague Dawley (SD) rats maintained in an animal house and fed with HFD (n=10) and Normal Diet (n=5). Lipid profiling within four different timelines was conducted using 3-in-1 combo test device for a complete lipid panel (Mission cholesterol meter) and Multi-Linear Regression (MLR) analysis performed using SPSS software. The obvious effects of High Fat Diet (HFD) shown in the unbalanced fluctuation of lipids level in SD rats from day 0 to day 90. According to MLR, the average mean of Low-Density Lipoprotein (LDL) and High-Density Lipoprotein (HDL) were in an increasing and decreasing patterns respectively. Meanwhile, triglyceride (TG) and total cholesterol (TC) were in an increasing and decreasing patterns respectively and each lipid in HFD were statistically correlated and significant. Unlike the control group, lipids level appeared to be in a constant manner. Our finding concluded that, dietary played a crucial role in the abnormality of lipid level which subsequently contributed to the occurrence of heart diseases