Published December 11, 2020 | Version v1
Journal article Open

INFLUENCE OF THE CRYOPRESERVATION ON THE VITALITY OF THE SPERM OF THE DIFFERENT BREEDS OF RAMS

Creators

  • 1. Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia, Bulgaria

Contributors

  • 1. University of Forestry, Sofia, Bulgaria

Description

The aim of the study was to determine the effect of cryopreservation on the vitality of sperm from dif-ferent breeds of rams. For the purpose of the study were used 15 healthy rams from three breeds – Ile-de-France, Lacaune, Synthetic Population of Bulgarian Milk (SPBM), during their insemination campaign. Sperm viability was determined by a Computer-assisted sperm analysis (CASA) after staining with a kit containing nigrosin-eosin (NE) solution. After cryopreservation, the highest percentage of vital sperm were found in the Ile-de-France breed. A significance difference was found between the Ile-de-France and SPBM breeds (P˂0.001) and between Ile-de-France and Lacaune (P˂0.01), regarding the studied indicator

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