Podospora anserina Niessl

EXPRESSION OF PEROXIDASE GENES IN P. ANSERINA MYCELIUM

We evaluated the expression of peroxidase genes during two days and four days of growth on M2 medium (Table 1), by analyzing microarrays and RNAseq data generated in previous studies (Bidard et al. 2012; Silar et al. 2019) (Table 1). We could evidence expression of all peroxidase genes (except for the “ Pa_2_10 ” pseudogene). The hybrid B peroxidase Pa_2_9370 and the aromatic peroxygenase Pa_0_370 genes were highly transcribed; on the contrary, the class II peroxidase Pa_1_5970 and the aromatic peroxygenase Pa_5_12205 gene were poorly expressed, a feature compatible with the lack of the aromatic peroxygenase Pa_5_ 1220 in secretomes data. Note that analysis of RNAseq data from perithecia showed that the class II peroxidase Pa_1_5970 gene was much more transcribed in these sexual fruiting bodies than in the mycelium, especially in four-day-old perithecia.