Molecular determination of Chlamydia trachomatis of infertile woman by 16SrRNA in Mosul City

Chlamydia trachomatis (CT) is the most common negative gram bacteria obligate intracellular pathogen that causes sexually transmitted diseases, including ocular trachoma worldwide. the objective was to diagnose genital CT infection among reproductive-age women attending Al-Batool Hospital in Mosul-Iraq. including Immuno-chromatographic test and quantitative Polymerase chain reaction (qPCR). A total of 125 females were included in this research, out of which 100 were infertile and 25 were fertile. Endo-cervical swabs were collected from all participants during the study period (July 2022 to September 2022). The immune chromatographic for chlamydia (ICT) and qPCR method was utilized to amplify and quantify chlamydial 16srRNA, known for its high specificity and sensitivity. qPCR is considered an ultrasensitive marker for the detection of CT. and was used to confirm the positive cases identified by ICT. Among the 125 women tested, a total of 12 cases (12%) were found to be positive for CT infection, all positive cases identified through immunographic test showed increased amplicon copy numbers with variable concentrations when analyzed by qPCR, providing strong evidence for the accuracy and reliability of molecular test. Out of the 100 infertile women, 12 were positive for CT infection, while none of the 25 fertile women tested positive. These findings suggest a potential association between CT infection and female infertility. The research demonstrated the utility of quantitative Polymerase chain reaction (qPCR) in diagnosing genital Chlamydia trachomatis infection among reproductive-age women.