Modifications of total synthesis of mycophenolic acid

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Introduction
2][3][4][5][6] However, the risks of rejection and side effects in the course of clinical treatment were not eliminated.][9][10][11][12][13][14][15][16][17][18] Although MPA is produced in industrial scale via fermentation processes, 19 its price for laboratory scale is still high.In the chemical literature are described attempts of total synthetic MPA from commercially available substrates.Some of them enable to prepare MPA analogs which are difficult to obtain by a modification of starting MPA molecule, since the structure of target derivative can be altered at the relevant synthetic stages.
In our research we decided to prepare some new MPA derivatives for examination of their immunosuppressive activity.1][22] In this article we report implemented in our work practical modifications of MPA synthesis.

Conclusion
Application of easy available tetralin as a solvent in the Claisen rearrangement of allyl ether 1 gave phenol derivative 2 with similar yield as obtained under conditions described by Patterson. 20][36][37] 1.
Alkene 3 (5.7 mmol) was dissolved in i-PrOH (30 mL) and potassium permanganate (16 mmol) was added portionwise.Then reaction was monitored with TLC technique (petroleum ether -ethyl acetate 10:1 v/v) and when starting material was consumed, sodium periodate (16 mmol) in water (5 mL) was added.The reaction mixture was stirred until the whole substrate reacted.Then, the reaction mixture was poured into water and extracted with ethyl acetate.The combined organic layers were washed with NaHCO3 until pH 7 was achieved, dried over MgSO4, filtered, evaporated under vacuum.The crude material was purified with column chromatography (petroleum ether -ethyl acetate 10:1 v/v) to give aldehyde 5 with 73 % yield.

Conflict of interest
There is no conflict of interest.