Authors:
Tianyi Zhou
;
Susan Perry
and
Svetlana Tatic-Lucic
Affiliation:
Lehigh University, United States
Keyword(s):
Viability, Hippocampal Neurons, Multi-Electrode Array (MEA), Dielectrophoresis (DEP), Sucrose, Membrane Potential.
Related
Ontology
Subjects/Areas/Topics:
Biomedical Engineering
;
Biomedical Instruments and Devices
;
Biomedical Sensors
;
Hardware
;
MEMS
;
Sensor Networks
;
Technologies Evaluation
Abstract:
In this work, we have investigated the viability of embryonic mouse hippocampal neurons after
dielectrophoretic positioning on multi-electrode arrays (MEA). We present a systematic evaluation of
positive dielectrophoretic conditions, including 1) an investigation of the effect of 10% sucrose (w/v in
deionized water), a commonly used, low-conductivity buffer medium, on the viability of mouse
hippocampal neurons over different time periods, and 2) a study of the effect of the membrane potential
induced by DEP electric field on the integrity of the cell membrane. Post-DEP high neuronal viability was
achieved experimentally, and spontaneous neuronal potentials from trapped neurons on the MEA were
successfully recorded.