Groundwater level, permeability and chemical components can be affected by earthquakes, however there are few comprehensive investigations on the combination of long-term continuous monitoring data and multiple strong earthquakes. In this study, continuous two-year dataset of groundwater levels and chemical compositions of groundwater (Ca²⁺, Mg²⁺ and HCO₃^-) in well #32 were collected to analyze the groundwater dynamic changes induced by earthquakes in the aquifer-aquitard system. The groundwater level appeared co-seismic rise change induced by Yangbi M_W 6.1 earthquake and Luding M_W 6.6 earthquake. The vertical permeability, estimated by the tidal response model, exhibited decrease changes during the period of Yangbi M_W 6.1 earthquake and Luding M_W 6.6 earthquake. Meanwhile, the continuous two-year chemical compositions showed that Ca²⁺ and HCO₃^- concentrations decreased, and Mg²⁺ concentrations increased during the two earthquakes period. The correlation between the vertical permeability and chemical compositions showed that there was a significant negative correlation between the vertical permeability and Mg²⁺, and a significant positive between the vertical permeability and Ca²⁺, HCO₃^-. A possible mechanism for observed fluctuations in some chemical compositions during earthquakes periods was that the reduction of mixing effect of different groundwater caused by permeability decreased. The flow of groundwater richened in Ca²⁺ and HCO₃^- from the overlying aquifer to the observation aquifer has been reduced. Meanwhile, due to the weakening of dilution effect, the Mg²⁺ concentration of the observation aquifer increased. This study can enhance understanding of the groundwater dynamic changes induced by earthquakes.