A Qualitative Baiting Technique for Selective Isolation and DNA Diagnosis of Rhizoctonia spp,Causal AGents of Rice Sheat Diseeases,from Soil - Collections

＜departmental bulletin paper＞
A Qualitative Baiting Technique for Selective Isolation and DNA Diagnosis of Rhizoctonia spp,Causal AGents of Rice Sheat Diseeases,from Soil

Creator	Author PID K001636 Creator Name Matsumoto, Masaru 松元, 賢マツモト, マサル Affiliation Affiliation Name Institute of Tropical Agriculture,Kyushu University
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2003-10-01
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	48
Issue	1/2
First Page	13
Last Page	20
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/4521
Related DOI	Journal of the Faculty of Agriculture, Kyushu University \|\| 48(1/2) \|\| p013-020
	九州大学大学院農学研究院紀要 \|\| 48(1/2) \|\| p013-020
	http://www.agr.kyushu-u.ac.jp/index.html
Related URI	Journal of the Faculty of Agriculture, Kyushu University \|\| 48(1/2) \|\| p013-020
	九州大学大学院農学研究院紀要 \|\| 48(1/2) \|\| p013-020
	http://www.agr.kyushu-u.ac.jp/index.html
Relation	Journal of the Faculty of Agriculture, Kyushu University \|\| 48(1/2) \|\| p013-020
	九州大学大学院農学研究院紀要 \|\| 48(1/2) \|\| p013-020
	http://www.agr.kyushu-u.ac.jp/index.html

File	FileType	Size	Views	Description
p013	pdf	638 KB	482

PISSN	0023-6152
NCID	AA00697606
Record ID	4521
Peer-Reviewed	Unrefereed
ISSN	0023-6152
NCID	AA00697606
Notes	A baiting technique was evolved for selective isolation and DNA diagnosis of casual agents of rice sheath diseases by Rhizoctonia spp. From artifically inoculated and naturally infested soils using eleven plant stem segments and selective medium containing benomyl and metalaxyl. Rice, mat rush and wheat stem segments soaked in metalaxyl at 50μg/ml were successfully used to isolate R. solani AG 1, R. solani AG 2, R. oryzae-sativae and R. fumigate from artificially infested soils. Moreover, rice and wheat system segments soused in metalaxyl at 100μg/ml were successfully used to extract genomic DNA derived from all Rhizoctonia spp. Metalaxyl untreated stems were colonized in significantly higher numbers and contained in significantly higher concentration of DNA of these Rhizoctonia isolates than untreated stems. Untreated stems were also colonized isolates and extracted DNA derived from other common soil-inhabiting fungi.
Created Date	2009.04.22
Modified Date	2022.11.11