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＜departmental bulletin paper＞
Complete Nucleotide Sequence of Bacillus sub tilis (na tto) Plasmid Responsible for γ-Polyglutamate Synthesis

Creator
Author PID
K001564
Creator Name
Hara, Toshio
原, 敏夫
Affiliation
Affiliation Name
Microbial Genetics Division, Institute of Genetic Resources, Faculuty of Agriculture, Kyushu University
九州大学農学部附属遺伝子資源研究センター
Creator Name
Yasuda, Shuhey
Affiliation
Affiliation Name
The Riken Institute
Author PID
L002941
Creator Name
Ogata, Seiya
緒方, 靖哉
Affiliation
Affiliation Name
Microbial Genetics Division, Institute of Genetic Resources, Faculuty of Agriculture, Kyushu University
九州大学農学部附属遺伝子資源研究センター
Creator Name
Soeda, Eiichi
添田, 栄一
Affiliation
Affiliation Name
The Riken Institute
理研
Creator Name
Ueda, Seinosuke
上田, 誠之助
Affiliation
Affiliation Name
Department of Applied Microbial Technology, Kumamoto Institute of Technology
熊本工業大学応用微生物工学科
Language
English
Publisher
Kyushu University
九州大学農学部
Date
1992-02
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
36
Issue
3/4
First Page
209
Last Page
218
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/23986
Related DOI
九州大学農学部紀要 || 36(3/4) || p209-218
Journal of the Faculty of Agriculture, Kyushu University || 36(3/4) || p209-218
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 36(3/4) || p209-218
Journal of the Faculty of Agriculture, Kyushu University || 36(3/4) || p209-218
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 36(3/4) || p209-218
Journal of the Faculty of Agriculture, Kyushu University || 36(3/4) || p209-218
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
23986
Peer-Reviewed
Unrefereed
Subject Terms
γ-Polyglutamate Synthesis
Bacillus sub tilis (na tto)
Nucleotide sequence
ISSN
0023-6152
NCID
AA00697606
Notes
The complete 5,812 base pairs nucleotide sequence has been determined for Bacillus subtilis (natto) plasmid pUH1, which encodes a ymglutamyltranspeptidase (EC 2.3.2.2) for synthesis of y-polyglutamate. Nucleotide sequence analysis reveals two long open reading frames coding for the gene for replication (rep) protein and y-glutamyltranspeptidase gene. Several putative regulatory sequences conserved were found upstream from these frames including the ShinemDalgarno sequence, Pribnow box and the “-35 region” at preferred distances for efficient transcription. The amino acid sequences predicted from the rep and ymglutamyltranspeptidase coding frames showed a significant homology, in each small segment, with those of E229 coded from the plasmid PC194 and of C403 from the plasmid pE194 of Staphylococcus aureus, respectively. However, no convincing homology of the pUH1 predicted ymglutamyltranspeptidase protein could be found with E. coli and mammalian ymglutamyltranspeptidases.
Created Date 2012.08.01
Modified Date 2022.11.11

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