Wearing virtual reality glasses are likely to cause visual fatigue and simulator sickness. In this research, effects of controller and body postures on simulator sickness and visual fatigue are studied by conducting experiments. 32 participants were asked to finish a series tasks while wearing virtual reality glasses. They were asked to fill in questionnaires before and after each experiment to measure visual fatigue and simulator sickness. As a result, participants in standing postures have more severe symptoms of visual fatigue than in sitting postures. And participants who use traditional controllers have more severe symptoms of visual fatigue and simulator sickness than those who use head-movement controller. The result may be useful in design of the interaction of virtual reality glasses.