The purpose of this study is to evaluate physiological and psychological stress in truck driver towing self-driving trucks. The truck A with which two self-driving truck B and C are coupled in series is run along a guide line expressway for running for about 30 minutes, 3times in a day. This experiment last 3 days, with 3 subjects derived the lead truck while towing 2 self-driving truck. For safety, driver was on the self-driving trucks B and C. In addition, each subject towing self-driving operation 3 times, solo operation (not towing self-driving) was 1 time. The bio-signal data (ECG, body temperature, skin temperature) of the subject were continuously measured at each driving phase. Subject's data phase "solo" compares with the towing data compared to detect the difference. As a result of those data, we have significant increase in skin temperature and heart rate between solo and towing operations.