Intensity of cytosol expression of 8-OHdG in normal renal tubules is associated with the severity of renal fibrosis

a Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan b Department of Nephrology, Buddhist Dalin Tzu Chi General Hospital, Chiayi, Taiwan c Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan d School of Medicine, Chung Shan Medical University, Taichung, Taiwan e Division of Nephrology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan


Introduction
Reactive oxygen species (ROS), composed of superoxide radicals, hydrogen peroxide and hydroxyl radicals, are involved not only in the aging process but are also either directly or indirectly implicated in a wide variety of clinical disorders, such as atherosclerosis, reperfusion injury, pulmonary toxicity, macular degeneration, cataractogenesis, diabetes, cancers and renal fibrosis [1][2].Although it is difficult to directly measure these free radicals because of a very short half life, products of radical damage to DNA, lipids, proteins and protective species are good markers of oxidative stress [3].Recent studies have focused on measuring 8-OHdG, which is a major DNA oxidation product, and its free base 8-hydroxyguanine (8-OH-G) in blood cells or urine as an important biomarker of oxidative DNA damage induced by ROS [4][5].8-OHdG, a metabolite of oxidative damage to leukocyte DNA, has been identified as a marker of oxidative stress in chronic renal patients on renal replacement therapy [5] and hypertensive patients with proteinuria [6].Scarring of the kidney, which is caused by a progressive fibrosis lead-ing to impairment of kidney function, occurs due to a variety of primary insults, such as diabetes mellitus, hypertension, primary glomerulopathies, autoimmune diseases, toxic injury or congenital abnormalities [7].High glucose, advanced glycation end products, angiotensin II and TGF-β1 are considered to increase intracellular ROS in renal cells and contribute to the development and progression of diabetic renal injury [2,8].The role of ROS in increased extracellular matrix (ECM) synthesis, which plays a critical role in glomerular mesangial expansion and tubulointerstitial fibrosis, has previously been reviewed [2,9].As ROS strongly correlate with ECM and tubulointerstitial fibrosis, we intended to study the relationship between renal expression of the ROS marker, 8-OHdG and renal fibrosis.Moreover, as we needed human renal tissues with various degrees of fibrosis, we performed this study using remnant tissues from nephrectomised kidneys instead of renal tissues from biopsies.

Materials and methods
From January 2006 to January 2009, pathological specimens from 74 patients who had undergone unilateral or bilateral nephrectomy were retrospectively recruited.The mean age of the patients at surgery was 60.6 ± 13.5 years.The indications for nephrectomy were urothelial cell car- cinoma (UCC) (n = 29; 39.2%), renal cell carcinoma (RCC) (n = 26; 35.1%), renal abscess or emphysematous pyelonephritis (n = 10; 13.5%), renal stones with or without hydronephrosis (n = 4; 5.4%) and others (including angiomyolipoma, renal cyst and primitive neuroectodermal tumour) (n = 5; 6.8%).Estimated glomerular filtration rate (eGFR) was calculated using the abbreviated Modification of Diet in Renal Disease formula (aMDRD) as follows: 186 × (sCr) -1.145 × (age) -0.203 × (0.742 if female), and the mean eGFR was 49.4 ± 31.1 ml/min.Chronic kidney disease (CKD) was defined according to the K/DOQI guidelines [10] and 47 patients (65.3%) were diagnosed with CKD.Among the patients with CKD, 12 patients had received renal replacement therapy (RRT) and the mean duration of RRT before operation was 6.6 years (range 3 to 10 years).This study was approved by the institutional review board at Chung Shan Medical University Hospital.Recipient age, gender, body mass index, systolic and diastolic blood pressure, laboratory and clinical data at operation including fasting glucose, cholesterol, triglycerides, haemoglobin and albumin were recorded.

Tissue processing
Pathological material was processed for conventional histological procedures.Representative sections were taken in the renal parenchyma at least 2 cm away from the tumour areas (in cases of nephrectomy due to tumour).Each section's dimension was at least 2 x 2 cm 2 .The formalinfixed, paraffin-embedded tissues were cut into 4-mm haematoxylin-and eosin-stained sections and reviewed to evaluate the glomerular, renal tubular and interstitial conditions.The scoring of fibrosis was based on Banff scoring for chronic lesions [11].To analyse the degree of fibrosis in the interstitium and glomeruli, we defined the low fibrosis group as a score of 0 or 1 and the high fibrosis group as a score of 2 or 3.

Immunohistochemical stain
Paraffin embedded kidney tissue sections (4-mm) on poly-1-lysine-coated slides were deparaffinised.After treatment with 3% H 2 O 2 in methanol, the sections were hydrated with gradient alcohol and PBS, incubated in 10 mM citrate buffer and finally heated at 100 °C for 20 min in PBS.After incubation with the 8-OHdG antibody [Santa Cruz, California, USA; 8-OHdG (clone 15A3)] for 20 min at room temperature, slides were incubated with a horseradish peroxidase (HRP)/Fab polymer conjugate for another 30 minutes.After being thoroughly washed three times with PBS, the sites of peroxidase activity were visualised using 3, 3-diamino-benzidine tetrahydrochloride as a substrate and haematoxylin as the counter stain.All of the interpretations of the immunohistochemical (IHC) data were scored by two pathologists blindly and independently and based on the method described as follows.Every specimen was sectioned 2 x 2 cm 2 and every slide was examined entirely by nuclear and cytoplasmic 8-OHdG stains in the normal and atrophic renal tubules, normal and atrophic glomeruli and tumorous parts.Those renal components available, including renal tubules, glomerulus or tumours were examined and graded.The number of immunoreactive cells was calculated semi-quantitatively and

Statistical analysis
Data were expressed as means ± standard deviation (SD).Categorical variables were analysed by the chi-square test and proportional comparison test if the renal specimens were not complete.The statistical significance between nonparametric variables was analysed by the Mann-Whitney U test.After evaluation by pathologists, there were 49 out of 74 specimens diagnosed as having different degrees of interstitial or glomerular fibrosis.We then analysed the correlations of clinical variables and 8-OHdG density of each renal component with interstitial and glomerular fibrosis by univariate linear regression analysis.Variables significantly associated with interstitial and glomerular fibrosis were tested for independence by stepwise multivariate linear regression (SMLR) analysis.A pvalue of less than 0.05 was considered statistically significant.The data were analysed using MedCalc version 11.2 statistical software.

Figure 2
Increased cytoplasmic expression of 8-OHdG in the residual normal tubules in fibrotic renal parenchyma (A) and adjacent to the fibrotic glomeruli (B).(IHC stain, 400x).
Stepwise multivariate linear regression was applied to evaluate the predictors of IFS and GFS, and the variables of serum creatinine, glucose and 8-OHdG intensity in NTc and in NTn were evaluated.The results demonstrated that serum creatinine (r = 0.351, p = 0.021; r = 0.563, p <0.001) and 8-OHdG intensity in NTc (r = 0.397, p = 0.01; r =

Original article
Swiss Med Wkly.2011;141:w13268 0.278, p = 0.043) were independent factors predicting IFS and GFS (table 5). Figure 2 shows the increased cytoplasmic expression of 8-OHdG with regard to the normal tubules in the fibrotic renal parenchyma and adjacent to the fibrotic glomeruli.

Discussion
In this study, the severity of renal fibrosis was demonstrated to be independently associated with the 8-OHdG expression in NTc and serum creatinine.Scarred kidneys are almost uniformly characterised by the triad of glomerulosclerosis, interstitial fibrosis and tubular atrophy [13].When renal injury occurs, glomerular or interstitial infiltrated inflammatory cells become activated and produce injurious molecules such as ROS, as well as fibrogenic and inflammatory cytokines [14][15].Huang et al. reported that the IHC location of 8-OHdG is on the nuclei of renal tubular and cystic epithelial cells in patients with ESRD [16].In our study, 8-OHdG was expressed predominantly in normal and atrophic renal tubular nuclei compared to tubular cytoplasm and mainly in the renal cellular nuclei of normal glomeruli rather than of atrophic glomeruli.It has been speculated that high glucose and TGF-β1 increase ECM synthesis and secretion.Additionally, they have been shown to decrease ECM degradation by inhibiting proteolytic systems, such as plasmin and matrix metalloproteases in the glomeruli, through ROS to induce tubulointerstitial fibrosis [2,15].Although our patients with The denominator represents the total number of specimen which contains the observation target components.Data were analyzed by the chi-square test and proportional comparison test if there were no complete components of renal specimen; p < 0.05 indicates significance.higher GFS or IFS tended to have more diabetes (p = 0.322, p = 0.283, respectively) [14,17], the blood glucose level was associated with glomerular or interstitial fibrosis in the univariate logistic regression analysis but was excluded after multivariate analysis.An overproduction of ROS and down-regulated expression of cellular antioxidant enzymes may play a pivotal role in

Original article
Swiss Med Wkly.2011;141:w13268 the mediation of glomerular, tubular, vascular and interstitial damage.[17] Fujii et al. reported that the myocardial (r = 0.848, p <0.001) and perivascular fibrosis (r = 0.906, p <0.001) scores in rats were significantly associated with the number of 8-OHdG positive cells [18].Oxidative stress measured by urinary 8-OHdG is believed to be a risk factor for both cardiovascular and renal disease, such as the association of detection of oxidative markers in the urine of diabetic patients and proteinuria [19][20].In addition, the phenomenon of cytoplasmic 8-OHdG staining in this study was similar to the report from Nomoto et al. that cytoplasmic fine granular 8-OHdG expression of hepatocytes in non-alcoholic fatty liver disease reflects 8-OHdG-positive mitochondrial DNA oxidative stress [21].Therefore, from the clinicopathological point of view, our results provide evidence that the expression of 8-OHdG in normal tubular cytoplasm could be an alternative pathological marker in mitochondrial damage and correlate with renal fibrosis.The occurrence of cancer has been reported to be associated with DNA damage.It has also been reported that the presence of nuclear 8-OHdG of hepatocytes is a significant risk factor for hepatocellular carcinoma, especially in patients with hepatitis C virus infection [22].Significant overexpression of 8-OHdG in DNA was observed from leukocytes in bladder cancer [23] and increased 8-OHdG ratio (8-OHdG level in RCC compared with that in normal tissue) was significantly associated with RCC prognostic indicators [24].Among our patients, the patients with higher GFS (p = 0.001) and IFS (p <0.001) were associated with a higher percentage of UCC.The average eGFR of the patients with UCC (34.8 ± 22.6 ml/min) was lower than that of the patients with RCC (67.3 ± 29.9 ml/min).This is because UCC is the most common malignancy in dialysis patients in Taiwan and chronic tubulointerstitial nephritis is the most likely underlying renal disease in haemodialysis patients with UCC [25].One concern is whether UCC or RCC affects the expression of 8-OHdG in adjacent renal tissue.Table 4 demonstrates that the pathological diagnosis of the nephrectomised kidney was not associated with either interstitial or glomerular fibrosis.The limitations of this study include the retrospective character, small sample size and the fact that some specimens did not include all the components of renal tissues.
In conclusion, our results provide evidence that cytosol expression of 8-OHdG in normal renal tubules is associated with renal glomerular and interstitial fibrosis.Further studies to clarify the molecular basis of this phenomenon are needed.

Figures (large format) Figure 1 High expression of 8 -
Figures (large format)

Figure 2
Figure 2Increased cytoplasmic expression of 8-OHdG in the residual normal tubules in fibrotic renal parenchyma (A) and adjacent to the fibrotic glomeruli (B).(IHC stain, 400x).

Table 1 :
Expression of 8-OHdG in different portions of kidneys.

Table 2 :
Characteristics of patients divided by low or high renal interstitial or glomerular fibrosis score.

Table 3 :
Association between fibrosis scores and expression of 8-OHdG in different portions of kidneys.

Table 4 :
Linear regression analyses on the associations between interstitial and glomerular fibrosis with clinicopathologic variables.

Table 5 :
Factors predicting interstitial or glomerular fibrosis by multivariate analysis using stepwise linear regression.Variables included serum creatinine, glucose, and 8-OHdG intensity in normal tubular cytoplasm (and normal tubular nuclei in interstitial fibrosis) *p < 0.05 was considered statistically significant in the stepwise multivariate linear regression analysis.
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