Fabrication of Curcumin Encapsulated Chitosan-PVA Silver Nanocomposite Films for Improved Antimicrobial Activity

Abstract

The present study explores the in situ fabrication of chitosan-poly(vinyl alcohol)-silver nanocomposite films in view of their increasing applications as antimicrobial packaging, wound dressing and antibacterial materials. The reduction of silver ions into silver nanoparticles (AgNPs) is achieved in acidic solution of chitosan (C) and poly (vinyl alcohol) (PVA) using their functional groups (-OH, -COOH, -NH2 groups). The presence of silver nanoparticles in the chito-san-PVA film is confirmed by UV-Vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy and X-ray Dif-fraction (XRD) analysis. The Scanning Electron Microscopic (SEM) images illustrate the presence of embedded silver nanoparticles throughout the films. In addition, the formed silver nanoparticles have an average particle size of ~ 16.5 nm as observed by Transmission Electron Microscopy (TEM). The anti-microbial and anti-fungal activity of the chitosan-PVA silver nanoparticle films have demonstrated significant effects against Escherichia coli (E. coli), Pseudomonas, Staphylococcus, Micrococcus, Candida albicans, and Pseudomonas aeruginosa (P. aeruginosa). To improve further their therapeutic efficacy as anti-microbial agents, curcumin encapsulated chitosan-PVA silver nanocomposite films are developed which showed enormous growth inhibition of E. coli compared to curcumin and chitosan-PVA silver nanoparticles film alone. Therefore, the present study clearly provides novel antimicrobial films which are potentially useful in preventing/treating infections.

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K. Vimala, M. Yallapu, K. Varaprasad, N. Reddy, S. Ravindra, N. Naidu and K. Raju, "Fabrication of Curcumin Encapsulated Chitosan-PVA Silver Nanocomposite Films for Improved Antimicrobial Activity," Journal of Biomaterials and Nanobiotechnology, Vol. 2 No. 1, 2011, pp. 55-64. doi: 10.4236/jbnb.2011.21008.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] D. G. Yu, M. Y. Teng, W. L. Chou, M. C. Yang, “Characterization and Inhibitory Effect of Antibacterial PAN Based Hollow Fiber Loaded with Silver Nitrate,” Journal of Membrane Science, Vol. 225, No. 1-2, November 2003, pp. 115-123. doi:10.1016/j.memsci.2003.08.010
[2] M. Rai, A.Yadav, A.Gade,“Silver Nanoparticles as A New Generation of Antimicrobial,” Biotechnology Advances, Vol. 27, No.1, January-February 2009, pp. 76-83. doi:10.1016/j.biotechadv.2008.09.002
[3] M. Catauro, M. G. Raucci, F. de Gaetano and A. Marotta, “Antibacterial and Bioactive Silver Containing Na2O?CaO?2SiO2 Glass Prepared by Sol Gel Method,” Journal of Materials Science: Materials in Medicine, Vol.15, No.7, July 2004, pp. 831-837. doi:10.1023/B:JMSM.0000032825.51052.00
[4] J. H. Crabtree, R. J. Burchette, R. A. Siddiqi, I. T. Huen, L. L. Handott, A. Fishman, “The Efficacy of Silver-Ion Implanted Catheters in Reducing Peritoneal Dialysis-Re- lated Infections, ” Peritoneal Dialysis International, Vol. 23, No.4, July- August 2003, pp.368-374.
[5] D.-C. Tien, K.-H. Tseng, C.-Y. Liao, T.-T. Tsung, “Identification and Quantification of Ionic Silver from Colloidal Silver Prepared by Electric Spark Discharge System and Its Antimicrobial Potency Study,” Journal of Alloys and Compounds, Vol. 473, No. 1-2, April 2009, pp. 298-302. doi:10.1016/j.jallcom.2008.05.063
[6] J. S. Kim, E. Kuk, K. N.Yu, J. H. Kim, S. J. Park, H. J Lee, S. H. Kim, Y. K. Park, Y. H. Park, C. Y. Hwang, Y. K. Kim, Y. S. Lee, D. H. Jeong, M. H. Cho, “Antimicrobial Effects of Silver Nanoparticles,” Nanomedicine: Nanotechnology, Biology and Medicine, Vol.3, No.1, March 2007, pp. 95-101. doi:10.1016/j.nano.2006.12.001
[7] R. Kumar, H. Munstedt, “Polyamide/Silver Antimicrobials: Effect of Crystallinity on the Silver Ion Release,” Polymer International, Vol. 54, No. 88, August 2005, pp. 1180-1186. doi:10.1002/pi.1828
[8] J. J. Castellano, S. M. Shafii, F.Ko, G. Donate, T. E. Wright, R. J. Mannari, W. G. Payne, D. J. Smith, M. C. Robson, “Comparative Evaluation of Silver Containing Antimicrobial Dressings and Drugs,” International Wound Journal, Vol. 4, No.2, June 2007, pp. 114-122. doi:10.1111/j.1742-481X.2007.00316.x
[9] M. Mishra, H. Kumar, K. Tripathi, “Diabetic Delayed Wound Healing and the Role of Silver Nanoparticles,” Digest Journa of Nanomaterials and Biostrctures, Vol. 3, No. 2, June 2008, pp. 49-54.
[10] Q. Li, S. Mahendra, D. Y. Lyon, L. Brunet, M. V. Liga, D. Li, P. J. Alvarez, “Antimicrobial Nanomaterials for Water Disinfection and Microbial Control: Potential Applications and Implications,’’ Water Research, Vol. 42, No. 18, November 2008, pp. 4591-4602. doi:10.1016/j.watres.2008.08.015
[11] R. Jung, Y. Kim, H. S. Kim, H. J. Jin, “Antimicrobial Properties of Hydrated Cellulose Membranes with Silver Nanoparticles,” Journal of Biomaterials Science: Polymer Edition, Vol. 20, No. 3, 2009, pp. 311-324. doi:10.1163/156856209X412182
[12] J. Jain, S. Arora, J. M. Rajwade, P. Omray, S. Khandelwal, K. M. Paknikar, “Silver Nanoparticles in Therapeutics: Development of an Antimicrobial Gel Formulation for Topical Use,” Molecular Pharmaceutics, Vol. 6, No. 5, May 2009, pp. 1388-1401. doi:10.1021/mp900056g
[13] A. Petica, S. Gavriliu, M. Lungu, N. Buruntea, C. Panzaru, “Colloidal Silver Solutions with Antimicrobial Properties,” Materials Science and Engineering: B. Vol. 152, No. 1-3, August 2008, pp. 22-27. doi:10.1016/j.mseb.2008.06.021
[14] S. K. Bajpai, Y.M. Mohan, M. Bajpai, R. Tankhiwale, V. Thomas, “Synthesis of Polymerstabilized Silver and Gold Nanostructures,” Journal of Nanoscience and Nanotechnology, Vol. 7, No. 9, September 2007, pp. 2994-3010. doi:10.1166/jnn.2007.911
[15] H. Kong, J. Jang, “Antibacterial Properties of Novel Poly(methyl methacrylate) Nano Fiber Containing Silver Nanoparticles,” Langmuir, Vol. 24, No.5, January 2008, pp. 2051-2056. doi:10.1021/la703085e
[16] V. K. Sharma, R. A. Yongard, Y. Lin, “Silver Nanoparticles: Green Synthesis and Their Antimicrobial Activities,” Advances in Colloid and Interface Science, Vol. 145, No. 1-2, January 2009, pp. 83-96. doi:10.1016/j.cis.2008.09.002
[17] V. Thomas, M. Namdeo, Y. Murali Mohan, S. K. Bajpai, M. Bajpai, “Review on Polymer Hydrogel and Microgel Metal Nanomposites: A Facile Nanotechnological Approach,” Journal of Macromolecular Science, Part A: Pure Applied Chemistry, Vol. 45, No. 1, January 2008, pp. 107-119. doi:10.1080/10601320701683470
[18] M. Zeng, Z. Fang, C. X, “Novel Method of Preparing Microporous Membrane by Selective Dissolution of Chitosan/Poly Ethylene Glycol Blend Membrane,” Journal of Applied Polymer Science, Vol. 91, No. 5, March 2004, pp. 2840-2847. doi:10.1002/app.13469
[19] H S. Kim, K H. Lee, S G. Kim, “Growth of Monodisperse Silver Nanoparticles in Polymer Matrix by Spray Pyrolysis,” Aerosol Science and Technology, Vol. 40, No. 7, July 2006, pp. 536-544. doi:10.1080/02786820600714361
[20] S. Liu, J. He, J. Xue, W. Ding, “Efficient Fabrication of Transparent Antimicrobial Poly (Vinyl Alcohol) Thin Films,” Journal Nanooparticle Research, Vol. 11, No.3, October 2007, pp. 553-560. doi:10.1007/s11051-007-9321-8
[21] S. Lu, W. Gao, H. Y. Gu,“Construction, Application and Biosafety of Silver Nanocrystalline Chitosan Wound Dressing,” Burns, Vol. 34, No. 5, August 2008, pp. 623-628. doi:10.1016/j.burns.2007.08.020
[22] A. M. Durango, N. F. F. Soares, S. Benevides, J. Teixeira, M. Carvalho, C. Wobeto, N. J. Andrade, “Development and Evaluation of an Edible Antimicrobial Film Based on Yam Starch and Chitosan,” Packaging Technology Science, Vol. 19, No. 1, January-February 2006, pp. 55-59. doi:10.1002/pts.713
[23] S. Tripathia, G. K. Mehrotra and P. K. Dutta, “PhysicoChemical and Bioctivity of Crosslinked Chitosan PVA Film for Food Packaging Applications,” International Journal of Biological Macromolecules, Vol. 45, No. 4, November 2009, pp. 372-376. doi:10.1016/j.ijbiomac.2009.07.006
[24] O. Suwantong, P. Opanasopit, U. Ruktanonchai, P. Supaphol, “Electrospun Cellulose Acetate Fiber Mats Containing Curcumin and Release Characteristic of the Herbal Substance,” Polymer, Vol. 48, No. 26, December 2007, pp. 7546-7557. doi:10.1016/j.polymer.2007.11.019
[25] S. K. Bajpai, Y. Murali Mohan, “Recent Advances in Nanoscience and Technology”, Bentham Science Publishers, Dubai, 2009.
[26] T. R. Thatiparti, A. Kano, A. Maruyama, A. Takahara, “Novel Silver-Loaded Semi-Interpenetrating Polymer Network Gel Films with Antibacterial Activity,” Journal of Polymer Science Part A: Polymer Chemistry, Vol. 47, No. 19, October 2009, pp.4950–4962. doi:10.1002/pola.23546
[27] A. Findon, G. McKay, H. S. Blair “Transport Studies for the Sorption of Copper Ions by Chitosan,” Journal of Environmental Science and Health. Part A, Environmental science and engineering, Vol. 28, No. 11, 1993, pp. 173-185. doi:10.1080/10934529309375870
[28] N. G. Kandile, A. S. Nasr, “Environment Friendly Modified Chitosan Hydrogels as A Matrix for Adsorption of Metal Ions, Synthesis and Characterization,” Carbohydrate Polymers, Vol. 78, No. 4, November 2009, pp 753-759. doi:10.1016/j.carbpol.2009.06.008
[29] K. Vimala, K. Samba Sivudu, Y. Murali Mohan, B. Sreedhar, K. Mohana Raju, “Controlled Silver Nanoparticles Synthesis in Semi Hydrogel Networks of Poly(Acrylamide) and Carbohydrates: A Rational Methodology for Antibacterial Application,” Carbohydrate Polymers, Vol. 75, No. 4, February 2009, pp. 463-471. doi:10.1016/j.carbpol.2008.08.009
[30] K.Vimala, Y. Murali Mohan, K. Samba Sivudu, K. Varaprasad, S. Ravindra, N. Narayana Reddy, Y. Padma, B. Sreedhar, K. MohanaRaju, “Fabrication of Porous Chitosan Films Impregnated with Silver Nanoparticles: A Facile Approach for Superior Antibacterial Applications,” Colloids and Surfaces B: Biointerfaces, Vol. 76, No. 1, March 2010, pp. 248-258. doi:10.1016/j.colsurfb.2009.10.044
[31] J. Ostrowska Czubenko, M. Gierszewska Dru?yńska, “Effect of Ionic Crosslinking on the Water State in Hydrogel Chitosan Membranes,” Carbohydrate Polymers, Vol. 7, No. 3, July 2009, pp.590-598. doi:10.1016/j.carbpol.2009.01.036
[32] S. Ekici, D. Saraydin, “Interpenetrating Polymeric Network Hydrogels for Potential Gastrointestinal Drug Release,” Polymer International, Vol. 56, November 2007, No. 11, pp. 1371-1377.
[33] V. Thomas, Y. Murali Mohan, B. Sreedhar, S. K. Bajpai, “Fabrication, Characterization of Chitosan/Nanosilver Film and Its Potential Antibacterial Application,” Journal of Biomaterials Science, Part A: Polymer Edition, Vol. 20, No.14, 2009, pp. 2129-2144.
[34] H. Hatcher, R. Planalp, J. Cho, F. M. Torti, S. V. Torti, “Curcumin: From Ancient Medicine to Current Clinical Trials,” Cellular and Molecular Life Sciences, Vol. 65, No. 11, June 2008, pp. 1631-1652. doi:10.1007/s00018-008-7452-4

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