Vegetative Propagation of Coleus forskohlii (Wild) Briq using their Stem Cutting for Ex-situ Conservation in Herbal Garden

Biodiversity includes variation and variability among the different living species of different natural habitats. Out of them plant diversity also placed in significant construction of biodiversity. Plant diversity is affected by several biotic and abiotic factors and is also regulated by their genes. Plant life is always affected by their population structure, natural effect in different phases of their life.


Introduction
Biodiversity includes variation and variability among the different living species of different natural habitats. Out of them plant diversity also placed in significant construction of biodiversity. Plant diversity is affected by several biotic and abiotic factors and is also regulated by their genes. Plant life is always affected by their population structure, natural effect in different phases of their life.
Interaction between environmental components and biota is important concern among the nature and living species. Each one species in nature willing to live long and to sustain in nature. Aimed for above reason the plants are forming numerous seeds after pollination and fertilization. A seed includes resting embryo between the cotyledons and are also protected by a cover known as seed coat. Seeds are variable in their size, shape, colour and weight etc. These are also unique for their germination tendency in the presence of favorable environmental condition. Resting phase of embryo in seeds is also variable in different plant species in nature.
Plants are remarkable source of a variety of products such as food, fodder, fuel, medicine etc. Due to presence of certain chemical compounds in plants these are performing their role in treatment of specific disorders. The group of the plants used for such purpose is referred as Medicinal and Aromatic Plants. Demand of these plants among the society is increasing day by day because of their rich potential of treatment, low cast and better effect.
Focusing on above points Conservation of these plants are of great concern in current situation over the world. To support the conservation process not only protection but their rapid propagation is also an urgent need so, plant/plant parts should be applied to regenerate them. Plants can be propagated either by seeds or by applying their vegetative parts including modified plant structures like bulb, tuber, rhizome and corm etc. All plants in nature are not equally capable to produce seeds but are well gifted by nature that they can propagate by their vegetative parts. The plant is also well adapted to regenerate using their stem cutting. Stem cutting made following proper length and mature stem focusing on the presence of nodes and small buds. The plant is marked for their wide range of propagation using both sexual and asexual modes.
The current is focusing the rapid vegetative propagation of Coleus forskohlii (Wild) Briq using their mature stem cutting aimed for their multiplication as well as for Ex-situ conservation in Herbal Garden. Kavitha et al. [1] found Coleus forskohlii: A comprehensive review on morphology. Ahmed and Vishwakarma [2] found Coleoside, a monoterpene glycoside from Coleus forskohlii. Kala

Abstract
Coleus forskohlii is a plant renowned for its medicinal as well as for aromatic values. It is medium heighted, herbaceous nature plant belonging to family Lamiaceae. Medium water supply is needed for this plants successful growth and development. This plant is also produces a beautiful violet coloured flower which further develops in to seeds of small size and round in shape. Mature stems are easily cut and found to be potential to convert in to its new small plants similar to their parental plants. The plant is well known for their propagation using their seeds and by stem cutting in the presence of favourable environmental conditions. The current paper deals with the study on its vegetative propagation using their mature stem cuttings in poly bags including sand, manure and soil mixture equally. Selected stem cuttings for the above purpose were allowed to grow in each one of the poly bags with medium water supply at the starting of the experimentation. Later on as per need of the developing plants facilities were given and managed to support the rich growth and development of the plant to make the experiment success. After development of the new plants these are carefully removed from poly bags and are further grown in the prepared beds in Herbal garden to multiply as well for ex-situ conservation. Related findings are discussed further more in this paper.

Materials and Methods
The plant Coleus forskohlii (Wild) Briq having efficient potential to develop new plants like their parental plants following by the seeds and by stem cuttings. The plant is well propagated using their mature stem cuttings. Each one cut part of the stem selected from mother plant based on their diseases free and maturity were further grown in directly in prepared beds.
For the same purpose there are fifty poly bags, sand, soil manure etc were collected. Mature plant parts/ stem cuttings were also selected and carefully removed from mother plant without damaging it.
Each poly bags were filled with sand, soil, manure and are further used for developing new plants of Coleus forskohlii (Wild) Briq using stem cuttings. Each one cut part of them included 4-6 buds and 10-15 cm long was cut from mother plant without damaging it. These stem cuttings were further used to develop the new plants following deeping in prepared fields and also in fifty poly bags. Well-developed plants in poly bags were shifted to required beds in Herbal garden (Figures 1-5). Proper monitoring was done to know the requirement of the developing plants in poly bags time to time. As per need of the plants facilities were provided.

Results and Discussion
The plant Coleus forskohlii (Wild) Briq is a herbaceous, green, branched herb of a great medicinal and aroma source. It includes many medicinal values giving it a rich value in market. Proper water facility and drainage system needed in the cultivated fields for avoiding water logging and to control on plant damages. Tap root present, branched. Stems are cylindrical, branched, hairy, upper part weak and green whereas below portion of the plant are solid in comparison to the top. Leaves are simple, small petiole, green, hairy, rough surface, dentate margin, thick and including aroma.
Flowers are small, attractive, blue-violet in colours and originating in a single flower axis. After pollination these are capable to forming several small, round seeds having potential to develop new individuals of this plant in favourable environmental conditions. Mixed media in poly begs performed their excellent combination which supports the easy and fast development of root as well as for shoot system in grown stem cuttings of Coleus forskohlii (Wild) Briq. With support of the favourable climatic condition Coleus forskohlii (Wild) Briq stem cuttings were started to develop new buds on their nodular part. These buds are further developing shoot system whereas roots are developing from nodular part of the stem at the base deep in soil in poly bags. Low temperature and high moisture level support the process of root and shoot development.
Finally it is concluded that the current study support the process of Coleus forskohlii (Wild) Briq rapid vegetative propagation in poly bags and is supporting their wide dissemination in required fields. This practice is helpful for Ex-situ conservation of this Medicinal plant Coleus forskohlii (Wild) Briq.