Nano-Silica Modified by Hematoporphyrin for Determination Some of Traces Metals Ions ( Cu ( II ) , Zn ( II ) and Mn ( II ) ) in Environmental and Pharmaceutical Formula

Recovering of heavy metal ions Zn(II), Cu(II) and Mn(II) in aqueous solution has been investigated using a novel nano silica modified by hemtaprophyrin. Nano sorbent was prepared by sol gel method. Transmission electron microscope and EDAX analysis showed the nano scale with porosity of a new sorbent. The proposed structure of modified silica with HP was predicted according to FTIR and elemental analysis. The new sorbent have good thermal stability. The phenomena of the sorbent sorption included the static studies such as pH and the Langmuir and Freundlish models were applied at equilibrium state. The linear regression analysis was Langmuir isotherm according to the values of relation coefficient (R2). The limit of detection (LOD) for Zn, Cu and Mn were 0.01, 0.12 and 0.03 μg L-1 respectively, within the analytical range 0.1-10 μgL-1. This method achieved highly precision for the samples of environmental and pharmaceutical formulations of % RSD less than 10%. *Corresponding author: Esam Bakeir, Department of Chemistry, College of Science, King Faisal University, Hofuf, Saudi Arabia, Tel: 00966509725364; E-mail: Esambakir99@yahoo.com Received July 15, 2017; Accepted July 22, 2017; Published July 27, 2017 Citation: Bakeir E, Attaf S, El-shahat M (2017) Nano-Silica Modified by Hematoporphyrin for Determination Some of Traces Metals Ions (Cu(II), Zn(II) and Mn(II)) in Environmental and Pharmaceutical Formula. J Environ Anal Toxicol 7: 495. doi: 10.4172/2161-0525.1000495 Copyright: © 2017 Bakeir E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Citation: Bakeir E, Attaf S, El-shahat M (2017) Nano-Silica Modified by Hematoporphyrin for Determination Some of Traces Metals Ions (Cu(II), Zn(II) and Mn(II)) in Environmental and Pharmaceutical Formula. J Environ Anal Toxicol 7: 495. doi: 10.4172/2161-0525.1000495


Introduction
Toxicity of heavy metal ions such as copper, Lead and zinc consider a big environmental pollution problem where there are many sources of this metal ions like soils, industrial wastes and geological samples [1] Flame and graphite furnace absorption spectrometry techniques used for determination concentration of this metal ions at low concentration directly [2].Low cost and efficiency of the use of solid phase extraction technology have become more common for the analysis of the components of samples in high and low concentrations.This technique can be automatically controlled and used in the pharmaceutical, environmental research and food industries [3].The disadvantage of some analytical techniques for example flame or graphite furnace atomic spectroscopy, inductively coupled plasma atomic emission spectroscopy(ICP-AES) and Inductively Coupled Plasma-Mass Spectroscopy (ICP-MS) for determination trace metal ions such as copper, zinc and nickel are less sensitivity for this metal ions with matrix interference so solving these problem, the preconcentration and separation techniques are used to maintain the limit of detection within the range of the proposed method.The development of the preconcentration and separation techniques is necessary [4][5][6].The most common of preconcentration methods is solid phase extraction(SPE) have several advantages over other methods [7][8][9] such as coprecipitation [10] ion exchange [11], solvent extraction [12].The efficiency of water treatment with removal trace metal ions from drinking, ground and wastewater carried out with selective preconcentration and separation processes [13].Functionalized sorbents have Features such as i) highly selectivity to targeted metal ion, ii) Overcome the difficulty of phase separation of the solubility between water and organic solvent iii) Determination the metal ions within part per billion concentrations, Iv) The adsorption of metal ions is visual for color complexes [14].The development of SPE materials for determination heavy metal ions incorporation inorganic or organic materials in matrices such as modified silica [15], cellulose [7], Amberlite XAD-4 modified with nano-manganese dioxide [16] activated carbon [17] polyurethane foam [18] and multiwall carbon nanotubes (MWCNTs) [19].
The most efficient sorbent for solid phase extraction (SPE) is modified silica gel incorporated with chelating organic ligand or inorganic compound to removal of metal ions due to high porosity, hydrophilicity and ease of surface modification.There are physically and chemically modified on the surface of silica gel using chelating organic ligand.The modification of silica gel surface using organic chelating agent increased the selectivity and sensitivity of SPE towards metal ions.Increasing the selectivity of silica sorbent by introduce donor atoms (O, N, P and S) of immobilized organic matter along the surface of silica and form a stable complex with uptake of the desired metal ions [20].there are many chelating ligands immobilized introduce on silica surface such as Silica gel functionalized with 1,8-dihydroxyanthraquinone [21], 2-aminomethylpyridine [22], p-dimethylaminobenzaldehyde, [23] have been used to enrich for metal ions.
The aim of this article, preparation modified silica with hematoporphyrin sorbent using sol gel method.FTIR, elemental analysis, thermal gravimetric analysis, transmission electron microscope and EDX techniques were studied the chemical structure, size diameter and thermal stability of desired sorbent.The adsorption parameters of determination of metal ion Zn(II), Cu(II) and Mn(II) using a nano sorbent have been studied at different pHs, with various shaking time, flow rate, type of eluent and interference of ions.Langmuir and freundlich isotherm models have been tested for the adsorption equilibrium of maximum adsorbed metal ions and kinetic analysis was examined the order of the reaction.The analytical validation (precision, accuracy and Limit of detection LOD) of this method was examined for water and pharmaceutical samples.
Nano-Silica Modified by Hematoporphyrin for Determination Some of Traces Metals Ions (Cu(II), Zn(II) and Mn(II)) in Environmental and Pharmaceutical Formula

Experimental
All solvents and reagents were highly analytical grade without purification such as Copper sulphate pentahydrate, Zinc sulfate heptahydrate, manganese sulfate pentahydrate and tetraethoxysilane purchased from Sigma-Aldrich.All glassware remained overnight in 10% (v/v) HNO 3 then washed several times with double-distilled water.A standard stock solution (100 mg L -1 ) of zinc, copper and nickel was prepared by dissolving the respective metal salts, and the working solutions were prepared by appropriate dilution with double-distilled water.Series of stock solutions of metal ions were used for plotting the calibration curve of the range 0-10.0 μg L -1 .Dilute Hydrochloric acid and sodium hydroxide were used to adjust the pH of the samples.There are three types of the extracted samples were used in adsorption process.

Olives samples
The samples were received from local market(Egypt), Cleaning, cutting then drying olive leaves in oven for 10 h at 80°C.Take 0.50 g of dried sample dissolve in concentrated HNO 3 and it dryness.The residue dissolved by double distilled water and neutralized with NaOH.The solution transferred into 50 ml measuring flask and filled with Distilled water.

Fish liver
The samples were received from local market (Egypt).Cleaning, cutting then drying the sample in an oven at 105°C for 24 h.Dry sample was ignited into a clean dried porcelain crucible for 2 h at 200°C gradually to 600°C in a muffle furnace.An accurate weighed 0.4 g of the final sample digested in HNO 3 till nearly dryness.The residue dissolved by double distilled water and neutralized with NaOH.The solution transferred into 50 ml measuring flask and filled with Distilled water.

Tap water
The samples were collected from sewage (chemistry dept.College of science, Ain shams university, Egypt).The sample was filtered to remove the suspended particles and adjusted pH=6.5 of samples by using 0.01 mol L -1 HNO 3 .

Pharmaceutical sampling
Pharmaceutical capsule of Kerovit was brought from Amoun Pharma Co (EGYPT).The sample of 1.768 g was dissolution into 20 mL of conc.HNO 3 then neutralized with NaOH and diluted to obtain 1 liter of sample.
The adsorption process of the samples was tested with nano sorbent HP-Silica which it prepared by sol gel method.From the BET data of nano sorbent HP-Silica given the surface area, average pore volume and pore radius were 176.490 m²/g and 0.097 cc/g and 7.173 nm respectively.

Procedure for adsorption experiment
The adsorption behavior of copper, nickel, zinc, cadmium and lead metal ions were examined by both batch and column conditions.The operational variables were optimized through batch process.The batch method was used to determine the adsorption capacity.The elution of the adsorbed metal ions was done in the column process.

Adsorbent preparation
Modified nano silica with hematoporphyrin was prepared sol gel method [24], Add 0.01 molar of hematoporphyrin to the solution of tetra ethoxy silane (TEOS), ethanol, H 2 O and hydrochloric acid (HCl) with molar ratio (1:2:4:0.01)respectively.The solution constantly stirring at room temperature for 1 h.The solution became gel form with homogenous.Dried gel at 110°C in an electric oven for 3 days.After nano HP-Silica was dried, it was stored for future experimental studies.

Batch process:
The uptake of metal ions was recorded for the sample volume 10 ml with different concentration ranges 0.5-30 μg L -1 of Zn(II), Cu(II) and Mn(II).The pH of analyte was adjusted by adding HCl or NaOH solution.The optimized pH of samples transferred in a clean conical flask containing 0.1 g of nano HP-Silica.The shaking time of solution was 1 h then filtered the solution using Whatman 41.The concentration of the filtrate solution was measured at flame atomic absorption spectroscopy (FAAS).The adsorption values of metal ions (Zn(II), Cu(II) and Mn(II)) was estimated by the eq (1): where C 0 and C e initial and equilibrium metal ions concentration (μg L -1 ) respectively, V(mL) is the volume of metal ions and m (g) is the weight of nno sorbent.
The removal percentage of metal ions (%) was estimated by eq (2): In batch process, there are several parameters were studied on the adsorption capacity of adsorbent such as pHs, adsorbate concentration, shaking time, eluent nature and its concentration.

Column preparation:
In the column experiments, 0.1 g of nano HP silica was packed in a thin syringe.The solutions of metal ions was adjusted to a proper pH and percolated with a certain flow rate vacuum pump.The adsorbed eluted with an appropriate eluent (0.1 mol.L -1 HNO 3 ) then determined their concentrations.

The column method:
The sample (5 mL sample volume with concentration 0.5 μg L -1 ) of metal ions was passed through the column and pH adjusted precondition passed the solution on glass column (3 cm long and 1.0 cm in diameter) was packed with 0.1 g of nano HPsilica beds help of silica wool at bottom.The flow rate was performed to the desired value determined experimentally (0.254.0 mL min -1 ).The adsorbed metal ions were recovered by passing 0.1 mol L -1 HNO 3 .The column washed many times with double distilled water.The adsorption and % removal of trace metal ions were estimated from the difference between the initial concentration of metal ions C 0 and the recovery concentration Ce according to Eq. ( 1) and ( 2).The flow rate and sample volume were optimized and tested in column.

Instrumentation
The IR spectrum of samples was recorded using a Perkin Elmer (USA) FT-IR Nicolet 6700 spectrophotometer.Thermal gravimetric analysis measured by TGA-50H (RASHA) analyzing.BET data were measured on NOVA-station.The adsorption concentration of metal ions were performed with FAAS (SOLAAR 3 Help©969 MKI) was used to determine the concentration of extracting metal ions.All pH measurements were measured with WTW pH meter.Scanning electron microscope (SEM) images were obtained from JEOL/ EQ JSM Instruments 1200 (Japan).Automatic analyzer CHNS, Elementar vario EL(III), Germany used to elemental analysis.

Characterization of nano HP-silica
Figure 1, show the spectra of a) pure TEOS have a wide band around at 3284 cm -1 refers to stretching vibration of O-H group of each Si-OH and H-OH bonding.A weaker O-H bending vibration band is recorded at 1653 cm -1 .The bands located at 465 and 1087 cm -1 for stretching vibration of Si-O-Si group The band's position at 1272, 1496 and 953 cm -1 are refer to the -CH 3 of the grafting of -Si-(CH 3 ) 3 groups attaching on the silica backbone, So the (CH 3 ) hydrophobic group attached to the silica backbone [25].The IR spectrum of HP incorporated in silica matrix, most of silica bands were observed with small shift and the stretching vibration band of (N-H) for hematoporphyrin was appeared at 2787 cm -1 [26] so the proposed mechanism of the reaction between HP and silica matrix is hydroxyl and carboxylic groups of hematoporphyrin attached to Si-OH of silica matrix.
Form Table 1 shown the % contents of %C and %H for HP-silica were higher than silica and also the %N was observed in new adsorbent nano HP-silica, this indicated to introduce HP on the surface backboned of silica.
Thermogravimetric analysis (TGA) was measured from 30 to 600°C for each silica and nano HP-silica sorbent.In Figure 2, the TGA curves shown a 15% and 5% weight loss ending up at 30-75°C for silica and HP-silica respectively, which is assigned to the adsorbed solvents (H 2 O, EtOH) from the condensation of silica network.And the 5% weight loss from 100-600°C is attributed to the pyrolysis of residual organics.The TG curve of the silica was observed no weight loss up to 300°C but for nano sorbent have weight loss 250-325°C expected decomposition of organic moiety of hematoporphyrin and without loss of weight to 500°C.

Morphological analysis (SEM) and compositional analysis (EDAX):
SEM-EDX also used to detect the quantity of silicon (Si) and oxygen (O) which are main composition of silica (SiO 2 ) and also detect the quantity of nitrogen (N) in silica gel modified HP-silica.Scanning electron microscope was used to analysis the surface morphology of silica powder and nano HP-silica sorbent as represented in Figures 3 and 4. The spherical and agglomerated silica nanoparticles shown the hematoporphyrin incorporated in the silica backbone with highly pore size.The EDAX analyses prove the successful synthesis of the silica material where Figure shows the Si/O ratio composition of silica and nano sorbent [27,28].TEM images show the size average of silica and nano sorbent was 8.0 nm and 7.4 nm respectively.The small size sorbent was made a high surface area and improved the extraction of metal ions from the solution [29].
BET analysis: Figure 5, shows the curve of adsorption-desorption isotherms of N 2 gas at surface of HP-silica sorbent were tested.Type II observed of the isotherm behaviour.The sample has a surface area of 176.50 m 2 /g, pore volume 0.097 cm 3 /g and pore radius 7.17 nm.The non-porosity nature of the sample has a lower BET surface area [30].

Batch method
The adsorption capacity of metal ions depend on several variables for example chemical nature of each sorbent and adsorbate, pHs values, shaking time, eluent type and it is concentration were tested on silica modified by hematoporphyrin.
Influence of solution pH: Figure 6 shows the adsorption capacity of metal ions(adsorbate) gradually increasing on the surface of modified silica by hematoporphyrin from acidic to neutral medium then decreasing in basic medium.At low pH, the surface of sorbent(HPsilica) was protonated with positive charge which it causing electrostatic repulsion with the metal ions (adsorbate) [31].the adsorption capacity gradually increasing till neutral medium because the complex formation take place between the metal ions and chelating organic moiety hematoporphyrin at the surface of silica.At higher pH, the metal ions were precipitated as M (OH) nor mixed charged hydroxide complexes.Precipitate made contamination on the surface of sorbent and hence a slight decline of the retention efficiency was achieved [32].The optimized pH of best % of recovery of the metal ions at pH=6-7.

Adsorption kinetic models
The minimum time period necessary to achieve maximum uptake of metal ions on the surface of modified silica by hematoporphyrin was tested the shaking time intervals 5, 10, 15, 20, 25 and 30 min.From Figure 7, The minimum time of maximum adsorption capacity was recorded for Zn(II) ion at time 20 min which it was less than the minimum time of maximum adsorption capacity of other metal ions.This indicated the fast sorption of diffusion of Zn(II) ion throughout a hypothetical or hydrodynamic boundary [33].
The kinetic mechanism controlling the adsorption of the metal ions was investigated by applying the pseudo first-order, pseudo-second order and intraparticle diffusion models to fit the experimental results.
The pseudo first order rate equation given by Lagergren and Kungliga [34] and supposed reaction between adsorbent and the adsorbate is monomolecular.
The linear equation plotted between log (q e -q t ) vs time t(min -1 ) shown in equation 3.
where q e and q t are the sorbet metal ion (μg g -1 ) at equilibrium, k 1 (rate constant)=2.303*slopeand time t (min), respectively.
The pseudo second order rate equation given by Ho [35] and supposed the reaction between the adsorbent and adsorbate is bimolecular.The linear relation given by equation 4 1 * e e t t q k q = (4) A plot of t/q t versus t should give a straight line if pseudo-secondorder kinetics are applicable and q e , k 2 and kq e 2 (mg g -1 min -1 ) can be determined from the slope and intercept of the plot, respectively where q e =1/slope and k 2 =(slope) 2 /Intercept.From Table 2 Validity the data of pseudo first-order model could be investigated by analyzing where the q e,cal values show increasing trends (111.43,59.06 and 80.18 μg g -1 ) for Zn(II), Cu(II) and Mn(II) respectively with correlation coefficients (R^2) 0.84-0.977and higher values of chi-square tests X^2 [36].Comparing the experimental q e values, a large difference was observed which proves the pseudo first order model is inconvenient for an interpretation of the adsorption kinetics data.
Validity the data of pseudo second-order model could be investigated by analyzing where q e,cal values show increasing trends (126.58,113.63 and 121.95 μg g -1 ) for Zn(II), Cu(II) and Mn(II) respectively with correlation coefficients (R^2) 0.988-0.992and lower values of chi-square tests X^2 [36].Furthermore, the calculated q e values from these second-order model were very close to experimental q e values.In the case of the pseudo-first-order model, the calculated q e values differed severely from the experimental q e values.

Intra-particle diffusion model:
The rate determine step of the reaction between the adsorbate and adsorbent was detected by the hypothesis of the intra-particle diffusion was investigated applying the Morris-Weber equation 5 [37].
where the intra-particle diffusion rate constant kid (μg g -1 min -1/2 ) was computed from the slope in the plot of q t values versus the squareroot of shaking time and intercept (I) represented contacted layer thickness.the plots (q t vs. t 1/2 ) represent multi-linearity, which indicates two or more steps occurring in the adsorption process.The relationship between q t vs. t 1/2 is the linear realation.The intra-particle diffusion constant was calculated using linear equation (Table 2).

Determination of isotherm adsorption model
Langmuir and Freundlich model interpreted the data of sorption equilibrium.All the isotherm model discussed in detail as follows [38,39]:

Metal ions q e (exp) µg g -1
Pseudo first-order Second order Second Intraparticle diffusion q e (µg g -1 ) k 1 (min -1 ) R 2 chi-square tests X 2 q e (µg g -1 ) k 2 (g µg -1 min -1 ) R 2 chi-square tests X 2 kid, 1 (µg g -1 min 0.5 ) kid, 2 (µg g -1 min 0.  (q K C ) q = (1+ K C ) (6) Linear equation where q e and K L are Langmuir constants representing the maximum adsorption capacity for the solid phase loading and the energy constant related to the heat of adsorption, respectively.It can be seen from Table 3 that the isotherm data fits the Langmuir equation well (R 2 =0.975-0.998).The values of q m and K L for Zn(II), Cu(II) and Mn(II) were determined from the Figure and were found to be 178.57,116.28 and 666.7 mg/g and 0.7616, 0.8032 and 1.0162/mg, respectively.The Freundlich isotherm model is an empirical relationship describing the adsorption of solutes from a liquid to a solid surface and assumes that different sites with several adsorption energies are involved.The Freundlich adsorption isotherm is the relationship between the amounts of lead adsorbed per unit mass of adsorbent (q e ) and the concentration of the lead at equilibrium (C e ).
1 * The logarithmic form of the equation becomes, where K f and n are the Freundlich constants characteristic of the system.K f and n are the indicator of the adsorption capacity and adsorption intensity, respectively.The ability of the Freundlich model to fit the experimental data was examined.For this case, the plot of log Ce vs log qe was employed to obtain from the intercept the value of K f and from the slope that of n.From Table 3, the Freundlich constants K f and n for Zn(II), Cu(II) and Mn(II) were found to be 2.48, 2.76 and 3.00 mg/g and 0.79,0.87 and 0.91 respectively.The isotherm data fit the Freundlich model well (R 2 =0.987, 0.976 and 0.992).Comparison of the obtained isothermal data is depicted in Table 3.The Langmiur and Freundlich regression coefficients proved that the correlation coefficients were stronger with respect to Langmuir model than the Freundlich model.Therefore, adsorption of the studied metal ions from aqueous solution onto Nano HP silica is matted well to the Langmuir isotherm model according to the R values.The maximum adsorption capacity obtained from the Langmuir isotherm was in agreement with the experimental values.

Sample flow rate
Sample flow rate is a measure of the contact time between the metal ion in the liquid phase and the solid sorbent.The lower the flow rates the longer the contact time and the larger the extent of metal binding.A vacuum pump was connected to the end of the syringe tap to adjust   The Langmuir model is assumed that monolayer of adsorbate molecules filled the surface of adsorbent with at maximum adsorption without any penetration for surface plane and the energy of adsorption the flow rate.An aliquot of 5 mL of concentration 0.5 μg mL -1 from individual metal ion was adjusted at pH 6-7 and introduced into the syringe at varying rates from 0.25 to 4.0 mL min -1 .Good results showed for Zn rather than other elements.At flow rate variations in the range from 0.25 to 2.0 ml min -1 , the recovery was approximately constant and quantitative.At flow rates higher than 2.0 mL min -1 , the recovery decreased very slowly.For achievement of a good precision, the flow rate was set at 1.0 mL min -1 in subsequent experiments.However, higher flow rates up to 2.0 mL min -1 could be employed when the sample concentration is too low to benefit from the decrease in analysis time but at the expense of efficiency.

Desorption metal ions
Eluent type can influence the lifetime of sorbent and efficiency of the method.The use of complexing agents like thiourea or EDTA was excluded since it might be sorbed by the Nano-HP silica, thus causing chemical changes in the nature of its surface leading to irreproducible recovery data.Hydrochloric and nitric acids are presumed to be the most suitable for desorption of the bound metal ions.The most frequently used is nitric acid because it is suitable with its non-oxidative effect.Universally, dilute mineral acids were found effective for removal of metal ions from Nano-HP silica based sorbents [40].However, at too low acid concentration the amount of protons may be not enough to protonate the chelation site and to make exchange with the retained metal ion.High acid concentration is not recommended for desorption due to increasing the volume of eluate by neutralization prior to FAAS determination that would reduce the preconcentration factor or contamination from acid.Therefore, concentration of nitric acid was carefully optimized.For this purpose, the metal ions were eluted from the column with 10 mL of HNO 3 solution at varying concentration between 0.1-0.5 mol L -1 with 1.0 mL min -1 flow rate.The recovery of Zn, Cu and Mn, was quantitative in the range 90-100% within concentration 0.1 mol L -1 .At acid concentration more than 0.1 mol L -1 , the recovery decreased which could be due to the synergistic effect caused by NO 3 -ion.Accordingly, the 0.1 mol L -1 concentrations were selected as the optimum eluent concentration in the subsequent experiments to achieve high recovery.The volume of eluent necessary to quantitatively remove these elements from the sorbent was investigated.

Breakthrough curve
The dynamic capacity of the column was computed by percolating it with a metal ion solution with concentration 0.5 μg mL -1 and pH 6.8 ± 0.4 at a flow rate 1.0 mL min -1 .Breakthrough curves were obtained by measuring the content of metal ions in 5 mL collected fractions from the effluent as shown in Figure 8. Evidently, the breakthrough point was found in 20, 15 and 10 mL for Zn, Cu and Mn, respectively.The zero-point sorption was reached after passing of 20 mL for Mn and 25 mL for Cu and Zn.The steep portions in the curves are of higher slopes, which reflect strong retention of metal ions to the sorbent.The working breakthrough capacity could be calculated by the equation: where C w is the column working capacity (µg g -1 ), V b is the collected volume of effluent between the first fraction and the breakthrough point (mL), C o is the initial concentration of metal ions, m is the weight of Sorbent (g).The capacity values were found to be 200 µgg - 1 for Mn(II) and 250 µgg -1 for Zn(II) and Cu(II), respectively.This revealed good retention of the element onto the column and predicted good preconcentration capability and low limit of detection.The breakthrough capacity is less than the total capacity determined from the extraction isotherm because the first one is determined under dynamic flow of metal ion solution where the contact time is short.On the other hand, the total capacity is determined under static conditions where enough contact time between the metal ion and the sorbent allows high mass transport to the solid phase.

Interference effect
The influences of some foreign ions which may interfere with sorption of the studied selements onto Nano-HP Silica were investigated in order to identify the method selected.For this purpose, 10 mL model solution containing 0.5 µg mL -1 Cu, Zn or Mn was mixed with the interfering species and adjusted to optimum pH and passed through the column at a flow rate 1.0 mL min -1 .The concentration of examined ions were: Na (1000 mg L -1 ), Ca(II) (500 mg L -1 ), Mg(II) (100 mg L -1 ), Fe (IIII) (0.1 mg L -1 ), Co(II) (0.5 mg L -1 ), Pb(II) (0.01 mg L -1 ), chloride (1000 mg L -1 ), sulphate (500 mg L -1 ), nitrate (1000 mg L -1 ), and oxalate (700 mg L -1 ).The obtained results are depicted in Figure 9. Strong interference was found by sulfate and lead on the Cu and Zn determinations, respectively.The alkaline and alkaline earth metal ions did not interfere with the extraction.Generally, the majority of the examined foreign ions were found to not interfere, which confirms the adequate selectivity of Nano-HP silica material and feasibility for quantitative determinations in natural samples with relevant accuracy.Furthermore, the added concentrations of foreign ions were higher than those existing in most natural samples which predict the validity of the developed procedure.

Influence of sample volume and preconcentration factor
The volume of the sample was directly related to the value of the preconcentration factor (PF).The high volume of the sample from which the metal ion can be quantitatively preconcentrated, the greater the value of PF.Therefore, the recovery of 0.5 μg of metal ions placed in solutions of varying volumes from 100 to 1000 mL was determined after desorption by 10 mL from 0.4 mol L -1 HNO 3 solution.The obtained results are summarized in Table 4. Quantitative recoveries (93-97%) were achieved at sample volumes in the range of 100-500 mL.Above 500 mL, the recoveries decreased and were not quantitative which might be attributed to incomplete retention of metal ions, probably by the washing action of the column by the sample itself.Therefore, a sample volume ≤ 500 mL was recommended for the simultaneous preconcentration of all metal ions in order to ensure good recovery.A preconcentration factor of range 12.4-29.5 was obtained by the ratio of the maximum sample volume to the eluent volume.

Reusability and precision
Reusability test was studied because it indicates the number of preconcentration cycles in which the column can be used without losing its analytical efficiency.Indeed, the whole experimental work was carried out on the same sorbent plugs either in batch or packed column methodology.There was no need to replace the plugs since it gave quantitative sorption when tested from time to time.Therefore, the reusability could be obtained by counting the total number of preconcentration cycles in which the plugs were used.It was found that the capacity of the sorbent was practically constant after its repeated use more than 70 cycles of preconcentration and desorption.The use of low concentration of eluent assisted extending the lifetime of the Sorbent and enabled the multiple use of Sorbent.The precision of the determination of these metal ions was evaluated under the optimum conditions for preconcentration and desorption by column method.Model solution was prepared by dilution of AAS standards to a concentration of 1.0 μg mL -1 each metal ion.The sample was subjected to preconcentration desorption cycle and the precision was estimated as the relative standard deviation (RSD) for five replicates.Results showed good precision (less than 10%) with RSD vary in the range 6.2-8.5%.

Accuracy
The add-found test was employed to evaluate the accuracy of the developed preconcentration method.Synthetic samples, containing individual metal ion at 0.5 µg mL -1 level, were prepared and passed into the column under the optimized conditions then the recovery and relative standard deviation RSD% values were found.The obtained results are compiled in Table 5. Quantitative recovery was achieved varying between 98.00 and 102.00% and the corresponding RSD values were in the range 0.20-1.02%.Obviously, the recovery is adequate with most measurements and RSD % is quite good.The results are in good agreement, showing that the proposed method might be used for metal

Analytical figures of merit
The analytical Figures of merit of the present procedure with determination by FAAS were evaluated.Linear calibration graphs have been obtained that could be represented by the regression equations: A=0.19+0.104C(R=0.999),A=-0.0123+0.10125C(R=0.999) and A=0.016+0.0515C(R=0.997) for Cu, Zn and Mn, respectively.The analytical range was found to be 0.1-10 μg L -1 .The detection limit defined as (3σ), where σ is the standard deviation of the blank determination, was found to be 0.12, 0.01 and 0.03 μg L -1 and the limit of quantification (10σ) was 0.41, 0.03 and 0.12 µg L -1 , respectively.Obviously, the limit of detections is less than the concentration of the tested metal ions in the majority of real samples which enables quantification of these elements with sufficient accuracy.

Analysis of real samples
The applicability of the developed sorbent was evaluated by analysis of several real samples.Firstly, the pharmaceutical preparation, Kerovit tablet, was analyzed.The obtained results are shown in Table 6.Adequate recovery values were obtained in the range 90.2-93.3%.The corresponding RSD values varied in the ranges 4.1-8.6%,which are reasonable.Secondly, the content of metal ions under investigation was determined in fish liver, olive leaves and drinking water.The obtained results are summarized in Table 7.The RSD % values are found to be in the range 3.7-9.7%which is considered relevant (less than 10%) for real samples.The obtained data conferred susceptible accuracy of the

Metal ion
Added a , µg mL -1 (mg) Found a , µg mL -   developed method based on the satisfactory values of RSD, in addition to the reasonably high sensitivity and validity of the proposed method, for determination in pharmaceutical and environmental samples.

Conclusion
Nano-sorbent prepared by simple and fast fabrication solgel method.The prepared sorbent was characterized by various microscopic and spectroscopic tools.It showed fast equilibration with the studied elements and easy regeneration.Novel sorbent was in a size of 7.4 nm of spherical shape.Hematoporphyrin was bonded from-COOH and-OH groups to surface of silica at-Si-O-Si and -OH.A good a preconcentration factor was achieved.The developed methods have been successfully applied for determination of metal ions in pharmaceutical and environmental samples.Nano-HP silica is highly selective for determination of Zn(II) rather than Cu(II) and Mn(II).Nano-HP silica was considered a perfect solid phase extraction with a good limit of detection and longer line arrange of concentration metal ions.This made the proposed procedure alternative to other reported solid phase extraction methods for application in pharmaceutical and environmental samples.

Figure 4 :Volume 7 •
Figure 4: SEM images and EDAX anlysis of the a) silica matrix and b) Nano-HP silica.A b

Figure 7 :
Figure 7: Effect of shaking time on adsorption of metal ions by nano-HP silica: metal ion concentration 0.5 μg mL -1 , sample volume (10 mL) and pH=6.8 ± 0.4, weight of sorbent 0.1 g and temperature 25°C.

Figure 9 :
Figure 9: Bar presentation of interference effect by some cations and anions on the recovery of Zn(II), C(II) and Mn(II).
even at low concentrations.Finally, the recovery percentage and RSD% values are extensively efficient for analytical determination of these elements at low concentrations.

Table 1 :
Elemental analysis (C, H and N) of nano silica and nano HP-Silica.

Table 2 :
Adsorption kinetic data obtained from pseudo-first order and pseudo-second-order models at 25°C.

Table 3 :
Langmiur and Freundlich isotherm constants for adsotption of metal ions on nano-HP silica at 25°C.

Table 4 :
Preconcentration of metal ions on 0.1 g nano-HP silica packed on column.

Table 5 :
Accuracy of the Nano-HP silica method calculated by add-found test.
a Mean value for five measurements

Table 6 :
Determination of Cu, Zn and Mn in the pharmaceutical sample Kerovit.
* Mean value of five replicate measurements (n=5)

Table 7 :
Determination of metal ions in environmental samples by the developed procedure by Nano-HP silica.