Prevalence of Bovine Trypanosomosis and Apparent Density of Tsetse Flies in Eastern Part of Dangur District, North Western Ethiopia

Trypanosomosis is a parasitic disease that causes serious economic losses in livestock, in sub-Saharan African countries. A cross sectional study was conducted from October 2011 to March 2012 in the eastern part of Dangur district, Benishangul-Gumuz regional state, Ethiopia to determine the prevalence of bovine trypanosomosis and apparent density of tsetse flies. For prevalence study, a total of 543 blood samples were collected from randomly selected animals. Packed Cell Volume (PCV) was determined and samples were examined for the presence of trypanosomes using the buffy coat technique. In total, 46 (8.5%) of the samples were tested positive for trypanosomes. The majority of the infections were caused by Trypanosoma congolense (95.7%), and the remaining was caused by Trypanosoma vivax. The difference between prevalence of trypanosomes among study sites was statistically significant (p<0.05). The mean PCV value of parasitemic animals (22.6%) was significantly lower (p<0.05) than that of aparasitemic animals (27.0%). A total of 528 tsetse flies were caught by deploying 78 monopyramidal traps. Of these tsetse flies, 71.8% were Glossina tachinoides and the remaining were G. morsitans submorsitans. The overall apparent density of tsetse flies was 3.4 flies per trap per day (F/T/D). In conclusion, this study revealed that trypanosomes and their vectors are prevalent and pose a huge threat to cattle production in the area. Therefore, proper intervention strategies should be put in place and implemented to minimize the burden of the disease. Prevalence of Bovine Trypanosomosis and Apparent Density of Tsetse Flies in Eastern Part of Dangur District, North Western Ethiopia


Introduction
Trypanosomosis is a disease complex caused by several species of blood and tissue dwelling protozoan parasites of the genus Trypanosoma [1][2][3]. It is a disease of domestic livestock that causes a significant negative impact on food and economic growth in many tropical and subtropical countries of the world including sub-Saharan Africa [4]. The course of the disease may run from an acute and rapidly fatal to a chronic long lasting one depending on the vector-parasitehost interactions. It is characterized mainly by intermittent fever, progressive anaemia and loss of condition of susceptible hosts which if untreated leads to high mortality rates [5,6].
The disease is distributed over approximately 10 million km 2 of Sub Saharan Africa between latitudes 14°N and 29°S which directly coincide with distributions of tsetse flies [2,7]. In Ethiopia, the most important tsetse born trypanosomes inflicting economic losses in domestic livestock are T. congolense, T. vivax, and T. brucei [8]. The distribution of tsetse flies is determined principally by climate and influenced by altitude, vegetation and presence of suitable hosts [9]. Five species of, tsetse flies, G. m. submorsitans, G. pallidipes, G. tachinoides, G. f. fuscipes and G. longipennis have been recorded in Ethiopia. Tsetse infested areas lie in lowlands and in the valleys of Abay (Blue Nile), Baro, Akobo, Didessa, Ghibe and Omo Rivers. The infestation is confined to the southern and western regions of Ethiopia between 33°-38°E and 5°-12° N which amounts to about 200,000 km 2 . Out of the nine administrative regions of Ethiopia, five (Amhara, Benishangul-Gumuz, Gambella, Oromia and Southern Nations and Nationalities and People Regional State (SNNPRS) are infested with more than one species of tsetse fly [8].
Although few studies were conducted in Northwestern Ethiopia, no study was conducted in Dangur district. Owing to the fact that, tsetse and trypanosomosis fronts in many places in Ethiopia are unstable and tsetse animal interface is constantly moving [10], studies on the epidemiology of trypanosomosis are crucial to plan and implement evidence based interventions. Aiming at filling the information gap in Dangur district, this study was conducted to determine the prevalence of trypanosomosis, identify trypanosome and tsetse species in cattle in the study areas.

Study area description
The study was conducted in 5 Kebeles (lowest administrative units in Ethiopia): Kitili, Burji, Gublak, Ipupuwa and Beles 2 of Dangur district, located 563 kms west of Addis Ababa in the Benishangul-Gumuz administrative region. Mixed agriculture is the mainstay of the livelihood of the society where crop and livestock production play integral roles. The district ( Figure 1) is situated at 11°18' N and 36°14' E with a total area of 838,700 hectares. It has an elevation that varies from 800 to 2000 meters above sea levels (masl) and has 70% plains, 8% valley and 22% mountainous topographic feature. The average annual low and high temperatures are 30°C and 38°C respectively and the mean annual rainfall ranges from 900 to 1400 ml. The dominant vegetations in the

Study animals
The study population constituted of indigenous zebu cattle managed under smallholder mixed crop-livestock farming system. The animals are kept under traditional extensive husbandry system with communal grazing and watering points [11]. Animal population of the district consists of 23,610 cattle, 7,945 sheep, 17,201 goats, 18 horses, 63 mules, 6194 donkeys and 43,448 poultry [11].

Sampling and sample size determination
A cross-sectional study design was conducted in dry season (November 2011-March 2012) to estimate the prevalence of trypanosomosis in cattle in the area. The study sites (kebeles) were selected based on their accessibility to transport. Sample size allocation was done based on the cattle population of the respective kebeles. Cattle owners were informed one day a head of sample collection to gather their animals at one place and simple random sampling technique was employed to select the study animals from the population. The sample size required was calculated at 50% prevalence with level of precision at 5% and 95% confidence interval using the formula described by Thrusfield [12]. As the actual prevalence was unknown, 50% was used to produce the largest sample size possible. Hence, a total of 384 animals were needed to be sampled. However, 543 animals were sampled to increase the precision of the study. Age, sex and body condition score of the studied animals were recorded during sampling. The age was estimated by means of their dentition [13]. The body condition status of selected animals was assessed and ranked as good, medium and poor [14].

Parasitological and hematological data
Blood samples were collected from superficial ear veins using sterilized lancet and heparinized micro-haematocrit capillary tubes. Immediately after blood collection, the tubes were sealed on one side with Cristaseal (Hawksley Ltd, Lancing, UK). The capillary tube was then transferred to a hematocrit centrifuge and spun for 5 min at 1200 revolutions per minute. The centrifuged capillary tube was measured on a hematocrit reader to estimate the Packed Cell Volume (PCV) as an indicator of anaemia. Then, the capillary tube was cut using a diamond tipped pen 1 mm below the buffy coat to include the uppermost layers of the red blood cells. The content of the capillary tube was expressed on to slide, homogenized onto a clean glass slide and covered with a 22 × 22 mm cover slip. The slide was examined using the 40 × objective for the movement of parasites [15]. Then Packed Cell Volume (PCV) was calculated using micro-haematocrit reader. For the purpose of species identification, thin blood smears were made and fixed with methanol for 3 minutes, stained with Giemsa stain for 30 minutes and examined under a microscope using the oil immersion 100 x objectives [16].

Entomological data
A total of 78 monopyramidal traps including 16, 20, 15, 6 and 21 were deployed in the riverside and wooded grassland areas of Kitili, Burji, Gublak, Ipupuwa and Beles 2 kebeles, respectively. The density and species of tsetse flies were assessed using odour-baited monopyramidal traps deployed at 200-250 m intervals. The odour baits used contained acetone, octanol and cow urine with appropriate apertures in order to release the necessary amounts of attractants. After 48 hours of trapping, the trap cage was collected [17]. The species and sex of the captured flies were identified based on morphological characteristics [18]. The

Data analysis
Raw data were entered into a Microsoft Excel spreadsheet and descriptive statistics were used to summarize the data. STATA ® version 11.0 statistical software programs were used to analyze the data. The point prevalence was calculated for all data as the number of infected individuals divided by the number of individuals examined and multiplied by 100. The association between the prevalence of trypanosome infection and risk factors were assessed by chi-square test (χ 2 ), whereas the two sample student's t-test was used to assess the difference in mean PCV between trypanosome positive and negative animals. The test result was considered significant when the calculated p-value was less than 0.05 at 95% confidence interval.

Parasitological results
A total of 543 animals were sampled including 123, 129, 70, 119 and 102 from Kitili, Burgi, Gublak, Ipupuwa and Beles 2 kebeles, respectively. Out of these, 46 (8.5%) were infected with trypanosomes. The majority (95.5%) of trypanosome species identified was T. congolense and the remaining 4.5% was T. vivax. Statistically different variation was observed in the infection status among different sites (Table 1, p=0.01). The prevalence of trypanosome in different age groups was 7.6%, 8.7% and 6.7% in young, adult and old animals respectively ( Table 2). The prevalence of trypanosomosis was not significantly different among age and sex groups. Of the 543 cattle examined, 97 (17.9%), 247 (45.5%) and 199 (36.7%) were in poor, medium and good body conditions, respectively. Higher proportion (18.3%) of cattle ranked as having poor body conditions were significantly more infected compared to those ranked as medium (8.1%) and good (4%) body conditions (Table 3, p=0.001).
The mean PCV value of the parasitemic animals (22.6%) was lower compared to the mean PCV value of aparastemic animals (26.9%) as indicated in Table 4. There was a statistically significant difference in mean PCV among parasitemic and aparasitemic animals (p=0.02). Besides, a total of 528 tsetse flies were caught ( Table 5). The majorities (71.8%) of the flies were G. tachinoides and the remaining were G. m. submorsitans.

Discussion
In this study, the overall prevalence of bovine trypanosomosis was 8.5% (95% CI=6.3-11.1). Similar findings were reported from different parts of Ethiopia. Earlier studies indicated the prevalence of bovine trypanososmosis ranging from 8.6 to 9% and from 6.6 to 11.3% in southwestern and north western parts of the country respectively [20][21][22][23][24][25].
However, our finding was higher than previous reports from districts in southern part of Ethiopia that showed the diseases prevalence ranging from 4.2 to 4.4% [26,27]. In these districts tsetse control has been carried out by the southern tsetse and trypanosomosis control project for many years which significantly reduced the prevalence. On the other hand, the current finding is lower than other reports of earlier studies in Ethiopia where the prevalence ranging from 17.3% to 28.1% were reported [28][29][30]. These variations could be attributed to seasonal differences during sampling periods and methods employed for the studies. The present study showed that the majority (95.5%) of the infections is caused by T. congolense and the remaining 5% is caused by T. vivax. The predominance of T. congolense in tsetse infested areas of Ethiopia has been reported by many authors. In Southwest Ethiopia, Abebe and Jobre reported an infection rate of 58%, 31.2% and 3.5% for T. congolense, T. brucei and T. Vivax, respectively [10]. Another study in south western Ethiopia recorded an infection rate of 37% for T. congolense [31]. The present finding is also supported by earlier works done in which 82.4% T. congolense and 5.9% T. vivax infections in Arbaminch, southern Ethiopia has been reported [26]. The predominance of T. congolense infection in cattle may be due to the high number of serodems of T. congolense as compared to T. vivax and the development of better immune response to T. vivax by the infected animal [9].     The trypanosome infection in male animals is slightly higher than in the female animals, but the variation was not statistically significant (p=0.21); showing that both male and female cattle were equally susceptible to trypanosomosis infection. This is in line with previous studies in Ethiopia [22,23].
There was a significant difference in trypanosome prevalence between the study kebeles (p=0.001). The high prevalence of the disease in Burji might have been attributed to the presence of relatively more suitable habitats (denser grassland and bush coverage) for the vectors compared to other areas. However in Beles 2 lower disease prevalence was observed despite a dense tsetse fly population. This could be attributed to the proximity of this Kebele to a veterinary clinic where the community has more easily access to animal health care compared to other areas. Treating animals with prophylactic drugs against the disease minimizes the prevalence of trypanosomosis in high tsetse fly population densities [32].
This study also showed that there is strong association between the body condition of cattle and trypanosome infection. The occurrence of infection was 4%, 8.1% and 18.6% in cattle with good, medium and poor body conditions, respectively. Thus, the majority of the infected animals manifest poor body conditions because of the effect of the disease. However, poor body condition could also be the consequence of other pathogens and nutritional stress [33]. The finding agrees with the reports of earlier studies in Ethiopia [21,28]. In this study, strong associations existed between the mean PCV value of the animals and occurrence of parasitaemia. The mean PCV value of aparastaemic (27.0%) was significantly higher than that of parastaemic animals (22.6%). The lower mean PCV value in parasitaemic animals than that of aparasitaemic ones was well recorded in previous studies in Ethiopia [34,35]. Another study conducted in southwestern Ethiopia indicated that in an increase in PCV value, the proportion of positivity decreases and hence mean PCV is a good indicator for the health status of the herd in endemic areas [36]. As anaemia is the classical sign of the disease pathogenicity [16], the low PCV in parasitaemic animals could have contributed in reducing the mean PCV of the cattle. The 8.5% overall prevalence of bovine trypanosomosis recorded in this study might not fully express the true extent of the disease burden because of the very low sensitivity and high variability of the parasite detection methods. Even though relatively high in acute state of infection, the sensitivity of buffy coat technique decreases over the course of the infection and becomes very low in chronic state of the disease [37]. A study conducted in Zambia also indicated that the buffy coat method fails to detect 66% of the infected animals [38]. However; the authors suggested that, the PCV-value of an individual animal is a good indicator of the presence of a trypanosomal infection. Therefore, the apparent parasitological prevalence of trypanosomosis is a little or much lower than the true parasitological prevalence in endemic areas. Hence, in endemic areas, it is necessary to complement the parasitological detection methods with PCR/RFLP and other sensitive molecular techniques to better understand the epidemiology of trypanosomosis and institute appropriate interventions.
The entomological survey revealed that tsetse fly species in the study area are G. m. submorsitans and G. tachinoides. In the study area, there is a typical habitat pattern for riverine species (G. tachinoides) along the rivers surrounded by savannah habitats suitable for G. m. submorsitans. Both of the identified fly species in the present study are among the five Glossina species recorded in Ethiopia [8]. The overall apparent density of tsetse flies was 3.38 F/T/D. Earlier studies in the western part of the country, reported the apparent density of Glossina species ranging from 0.3 to 24.4 F/T/D [26,39,40]. Such wide variations could have been resulted from differences in season and density of vegetation cover and types of traps deployed, type and volume of odour attractants utilized during the studies. The low density of tsetse in the study area may have been due to the expansion of settlements and farmlands in the area. It may also be explained by the migration of the game as a result of climate and habitat changes [9]. The relative abundance of G. tachinoides (71.8%) than G. m. submorsitans (28.2%) might have been due to ability of this species to adapt to unsuitable habitats. Riverine flies appear to be largely unaffected by human population density and can even adapt to human-made environments [41].

Conclusion
In conclusion, T. congolense is the predominant species of trypanosome in the study area although T. vivax was also present. Tsetse fly species caught in the study area were G. m. submorsitans and G. tachinoides. This study also indicated that infection with trypanosomosis negatively affects the body condition and PCV profile of animals. Taken together, tsetse borne trypanosomosis is posing a considerable threat to cattle production in Dangur district, western Ethiopia. Therefore, it is imperative to extend and strengthen the national tsetse and trypanosomosis control scheme in tsetse infested areas in Ethiopia to minimize the burden of the disease.