Recent Advances and Applications of Biosensors in Novel Technology

In recent time, several novel biosensors such as enzyme based, immunosensors, tissue-based, DNA biosensors, piezoelectric, and thermal biosensors have been explored with high sensitivity by many research groups. These biosensors exhibit many essential benefits, including operational simplicity, remarkable sensitivity, low-cost instrumentation, the potential of automation, inherent miniaturization. They have offered elegant paths for the detection of even trace amounts of analytical agents of biological significance existing from macromolecules (e.g., disease biomarkers and antigens) and small molecules (e.g., natural toxins and haptens) to cells, viruses, and bacteria. Many electrochemical techniques viz., amperometry and voltammetry, photoelectrochemistry, electrochemiluminescence, impedance, piezoelectricity, potentiometry, alternating current electrohydrodynamics, and field-effect transistor have been utilized in the development of immunoassays to attain high sensitivity for electrochemical signal transduction. Recent developments in biological methods and instrumentation after using fluorescence tag to various nanocarriers such as nanoparticles, nanowires, nanotubes, etc. have improved the sensitivity of biosensors. Utilization of nucleotides/aptamers, affibodies, molecule imprinted polymers, and peptide arrays offer great tools to prepare advanced biosensors. Merging of nanotechnology with biosensor systems enhanced the diagnostic capability. This review gives an outline of the advances in biosensors technology relating biomedical sciences.


Introduction
The biosensor can be defined as a device that utilizes biological molecules or living organisms such as antibodies and enzymes to identify chemicals. The biosensor was first introduced in the 1960s and described the application of enzyme based bioelectrodes and their biocatalytic action. Afterward, in 1962, Clark presented a biosensor based model involving an oxygen electrode for the detection of electrochemical such as hydrogen peroxide or oxygen for the bioanalytical application. Later, the use of biosensors has expanded in the frontier of interdisciplinary research, i.e., analytical chemistry, bioelectronics, combines biology, and physics [1]. Different types of biosensors are being employed, which includes enzyme based, immunosensors, tissue-based, DNA biosensors, piezoelectric, and thermal biosensors [2].
The biosensor is a potential analytical device, which is used as a biological element (e.g., cell receptors, tissue, organelles, enzymes, microorganisms, nucleic acids, antibodies, etc.) to interacts (i.e., recognizes or binds) with an analyte [3]. A biosensor usually consists of three elements; a biorecognition, biotransducer, and an electronic system that include a processor, signal amplifier, and display [4,5]. The recognition part often termed as a bioreceptor, which exploits biomolecules as receptors to interact with a specific analyte. High selectivity of the analyte with bioreceptor is necessary. Biosensors are classified according to their types of interactions between biosensor and analyte, i.e., antibody/antigen, nucleic acids/DNA, enzymes/ ligands, biomimetic materials or cellular structures/cells [6].
Biosensors exhibit widespread applications in numerous field viz., in novel drug discovery, biomedicine, diagnosis, food safety and processing, environmental monitoring, security, and defense. Pharmaceutical residues have conventionally been detected via qualitative/semi-quantitative screening techniques. Earlier, only distrusted samples were analyzed for validation by chromatographic techniques, i.e., high-performance liquid chromatography (HPLC) or gas chromatography (GC) attached to mass spectrometry (MS). Afterward, most of the pharmaceutical molecules have been derivatized to achieve good volatility for testing via gas chromatography. Nowadays HPLC-MS has shown wide acceptance (e.g., estrogens and antibiotics) in the analysis. For instance, capillary electrophoresis (CE) is another technique, which has not been accepted widely for analysis owing to its lack sensitivity over HPLC. Current advances in sample-preparation methods, detectors, and other apparatuses have enhanced the limits of detection (LODs). Instead, immunochemical methods with high-sample throughput ability could be used to achieve demands of pharmaceutical examinations [7].
The miniaturization of electrochemical immunosensors on microfluidic stages can not only overcome the limitations of conventional methods, including long detection times and the use of large volumes of reagents but also expand the use of immunosensors as a point of care tools [8][9][10].

Types of Bioreceptors Enzymatic interactions
The enzyme-based sensor was first-time described in 1967 by Updike and Hicks [11]. In enzymatic interactions, initially, the substrates molecules, which is specific to an enzyme, bind to the enzyme and converts this complex into products. The mostly used bioreceptor include glucose oxidase (GOD), polyphenol oxidase (PPO), and urease. The enzymatic interactions exhibit some advantages such as; are highly selective, show catalytic activity, which improving sensitivity, and are fast acting. Some limitations are also associated with this are expensive (i.e., high cost of the source, extracting, isolating, and purifying), the activity might be lost during immobilization on a transducer, loss of activity owing to deactivation after a short period of time (due to storage in non-appropriate conditions).
Catalytic activity and explicit binding of enzyme make them potential bioreceptors. Analyte identification is enabled via several mechanisms: a) enzyme activation/inhibition is detected via the analyte; b) conversion of the analyte to a final product by the enzyme, which is detected by the sensor, or c) modifications in enzyme properties, which are occurring due to enzyme-analyte interaction is monitored [12]. The use of enzymes as a biosensor has some benefits such as: b) they can recognize a wide range of analytes (substrates, modulators, products, and inhibitors); a) they have the capability to catalyze several of reactions; and c) they are suitable for different transduction approaches to detect the analyte. Especially, enzymes are not used up in reactions. Hence biosensor can be utilized unceasingly. Though, biosensors have limited lifespan owing to limited enzymes stability.
The tyrosinase (Tyr), as an immunoassay label, can convert a weakly electroactive phenol to a highly electroactive catechol to trigger a βnicotinamide adenine dinucleotide disodium salt (NADH)-related catalysis, which leads to a high signal-to-noise ratio for the immunoassay [13]. To minimize electrochemical signal interference, Yang and coworkers designed a washing-free electrochemical immunosensor with glycerol-3-phosphate dehydrogenase (GPDH) as an enzyme label. Because the reaction of GPDH with dissolved oxygen is low and the electrochemical interference of Ru(NH 3 )6 2+, an electrochemical oxidation product generated by GPDH, is also very low [14]. Xu et al. developed a highly sensitive electrochemical aptasensor by using nanocarriers of porous platinum nanotubes grafted with polyamidoamine based dendrimers as electrocatalysts for detection of thrombin. The enzyme-based signal amplification results showed a low detection limit, i.e., 0.03 pM [15].

Antibody/antigen interactions
Antibodies (Ab) or immunoglobulins proteins can bind to a foreign antigen (Ag) and assist in neutralizing foreign/invading particles.

Ab + Ag ↔ Ab•Ag
Antibodies-antigens interaction is very selective and ultra-sensitive, they can bind with high power (Ka=106). Despite advantages, it presents some limitations also such as no catalytic effect is seen, only one-time use of biosensor is possible (disposable strips). Immunosensors, for instance, antibodies, utilize high binding affinity for a specific molecule or antigen. This specific property of antibodyantigen interaction is similar to the lock and key fit where antigen binds only to a specific antibody. This linking result into a physicochemical alteration in enzymes, radioisotopes, or fluorescent molecules, which produce a signal. Some limitations that include are; the antibody-antigen interaction is usually irreversible, and the binding capacity of an antibody is extremely dependent on conditions of the assay (e.g., temperature and pH). However, organic solvents, chaotropic reagents, and ultrasonic radiation can disrupt antibody/ antigen interaction [12]. In an approach, a small protein with promising biophysical characteristics has been generated as antigenbinding proteins with the specific binding ability to target various proteins without affecting properties of the molecule. Selection of elements, which can specifically attach to a target antigen, was performed (in vitro) via phage display, yeast display, ribosome display, or mRNA display. However, artificial proteins for binding are usually smaller (i.e., <100 amino-acid residues) than antibodies. They have high stability with deficiency of disulfide bonds in contrast to antibodies [16,17]. Artificial proteins are thus a promising candidate to create biosensors [18,19]. Li et al. studied a non-enzymatic immunosensor as an important tool for the detection of carbohydrate antigen 15-3 using hierarchical nanoporous PtFe alloy [20,21].

Nucleic acid interactions
The nucleic acid is the building blocks of genetics. It operates selectively owing to base-pairing features, If the sequence of bases containing certain known part of the nucleic acid molecule, then the complementary sequence of it can be synthesized (called as a probe) and labeled using an optically detectable agent (eg., a fluorescent label). It has shown great potential in the detection of several types of cancers [22,23], viral [24] infections, and genetic [25] disorders. The nucleic acid biosensors are developed on the principle that the singlestrand of the nucleic acid molecule has the capability to identify and bind to its complementary strand. The interaction occurs owing to the formation of hydrogen bonds between the strands of two nucleic acids [26].
Biosensors that interact with nucleic acid are called as genosensors. In this type of interaction complementary base-pairing principle is used for recognition for example in DNA; adenine-thymine and cytosine-guanine. Complementary sequences are synthesized using known target nucleic acid sequence, labeled, and afterward immobilized on the sensor. Hybridization probes form a base pair with target sequences and thus generate optical signals. These optical signals are detected and favor transduction principle [12]. Song et al. induced DNA hybridization (in situ) via utilizing silver NPs aggregate as an electrochemical aptasensor (disposable) for signal amplification [27,28]. In another study, a gold nanomaterial and graphene based DNA biosensor showed high sensitivity [29]. Khimji et al. developed DNA-functionalized polyacrylamide hydrogels based optical biosensors [30]. Several DNA based biosensor utilizing NPs are being attach to the surface to facilitate immobilization. Cells can persist their activity for an extended period over organelles. They are usually utilized to monitor treatment results of drugs and can detect various parameters viz. toxicity, organic derivatives, and stress condition. Cells are employed to control herbicides, which are a main aquatic pollutant [34]. In another cell application, quartz microfiber is used to entrap micro algae, and then chlorophyll fluorescence is modified via the herbicides, which is collected from the tip of a bundle of the optical fiber and detected through a fluorimeter. Results reveal that certain herbicide detection limit can be reached up to sub-ppb concentration level. microbial corrosion is also monitored by using few cells [35]. Organelles inside a cell perform distinct functions independently. Organelles using specific enzymes carry out various essential metabolic pathways and fulfill body need. Some typical organelles include mitochondria, chloroplast, and lysosome. Mitochondria exhibits a substantial role in calcium ion metabolism to control functioning and modulating the calcium associated signaling pathways. It also presents some advantages such as sometimes it shows longer response times and recovery times, substrate requires to diffuse into the cytoplasm, and have less selectivity (as demonstrated by tissues owing to many enzymes). Elsewhere results revealed that mitochondria hold potential to respond high concentration of calcium that generates inside the proximity owing the opening of the calcium channel [36]. Thus, mitochondria are utilized to detect calcium concentration with high sensitivity (e.g., in water pollution detection) [37].
Tissues are employed as a biosensor for the plenty of enzymes. Some important advantages of tissues as biosensor include [38]: a) tissues can be immobilized easily as compared to organelles or cells, b) essential cofactors already exist for well-functioning of enzyme, c) Low cost and easy availability, d) the extraction, centrifugation, and purification of enzymes is not essential, e) a wide range of possibilities are available to fulfil different objectives, and f) in natural environment, enzymes show higher activity and stability. Despite advantages, tissues show some disadvantages such as they show less selectivity as compared to purified enzymes since they contain a variety of enzymes, reduced substrate specificity, slower response time, require more tissue material as a substrate to diffuse through, and this decrease the effect of enzymes.

Epigenetics
The term epigenetics in its contemporary application emerged in the 1990s; Epigenetic is the stably heritable phenotype resulting from variations in an organism that is caused due to modification of gene expression instead of a change in the genetic code itself. Photonic biosensors have been developed, which can detect tumor cell in a urine sample to an ultra-sensitivity level [39]. Elsewhere, epigenetic modifications are detected after exploitation of integrated optical resonators (e.g., post-translational modifications in histone and DNA methylation) using body fluids of patients suffering from cancer or other ailments [40].

Surface Attachment of Biological Elements
A vital step in relating to biosensor is linking of biological elements (i.e., cells/small molecules/proteins) on the surface of the sensor (e.g., polymer, glass, or metal,). However, the simplest method of the surface attachment is the functionalization of the surface via coating with the biological element. This is achieved by epoxysilane, aminosilane, nitrocellulose, or polylysine. Afterward, the attached biological molecules can be fixed via Layer-by-layer deposition using coatings of charged polymer [41]. Instead, 3-dimensional lattices (xerogel/ hydrogel) can be employed to entrap these biological agents by chemical/physical method. The commonly utilized hydrogel is glassy silica (i.e., sol-gel), which is developed after polymerization of monomers of a silicate such as-tetra alkyl orthosilicates (e.g., TMOS/ TEOS) in the presence of biological elements and PEG [42].

Placement of biosensors
The suitable positioning of biosensors is decided to owe their field of applications viz. biotechnology, biomedicine, food technology, and agriculture. In biomedical applications, biosensors are usually categorized according to in vitro or in vivo application. In vitro biosensor investigations are performed inside a test tube, a microtiter plate, a culture dish, or somewhere else outside the living animal. The sensor includes a bioreceptor and a transducer. Enzymeconductimetric based biosensor is a good example of in vitro type of biosensor utilized for determination of blood glucose level [43]. The exclusion of lab examination can save both time and money. However, POCT biosensor is utilized for confirming HIV virus, where it is not possible to test patients. Now it is easier to perform quick and easy testing using a biosensor. Whereas, a biosensor for in vivo is a device, which is implanted within the body. Hence, in vivo biosensor used for implants must be sterilized fulfill all the strict regulations to avoid primary inflammatory response [44].

Types of Biotransducer
Biosensors are classified as: a) electrochemical biosensors, b) optical biosensors, c) electronic biosensors, d) piezoelectric biosensors, e) gravimetric biosensors, and f) pyroelectric biosensors. Few important biosensors are described here.

Electrochemical
Electrochemical biosensors usually worked on the principle of enzymatic catalysis that generates or uses electrons (i.e., redox enzymes). Sensor substrate generally includes three electrodes; a) a reference electrode, b) a working electrode, and c) a counter electrode. The target analyte, which is used found on the electrode active surface, and the reaction may result in either transfer of electron across the double layer or can produce a potential to the double layer. The flow rate of electrons is directly proportional to the concentration of the analyte [45]. Potentiometric biosensor produces a logarithmic response. These biosensors are prepared using screen printing of electrode patterns over the surface of the plastic substrate and coated using the conducting polymer attached to some enzyme or antibody. All biosensors generally require very less quantity of sample since biological sensors are highly selective for the analyte. The signal is generated owing to physical and electrochemical variations in the layer of conducting polymer owing to changes over the surface of the sensor. These variations can be ascribed to pH, redox reactions, ionic strength, and hydration. Wang et al. studied the role of non-mediating ultrasensitive electrochemical immunosensors in Au/Ag/Au core/double shell NPs as enzyme-mimetic labels [46].

Electrochemical immunosensor
Many electrochemical immunosensor utilizing novel carrier systems have been developed for instance; carbon nanotube/manganese dioxide [47], graphene and Cu@Ag core-shell NPs [48], gold NPs [49]. Horseradish peroxidase and ferroferric oxide were used as a signal

Impedimetric biosensors
Impedimetric devices permit direct recognition of biomolecular activities without utilizing enzyme. In the biomedical investigation, impedimetric biosensors are widely employed in the label-free recognition of the ciprofloxacin (an antibiotic drug) at a very low concentration, (
Nanostructure based transducer surfaces have attracted in the recent progress of biosensors. These biosensors offer enhanced selectivity and sensitivity over existing techniques. Kim and coworkers prepared a chloramphenicol containing amperometric immunosensor using cadmium sulfide nanoparticles and modified using dendrimer bonded to the conducting polymer (AuNP) [64]. An amperometric immunosensor (label-free) utilizing Ag-NPs decorated with infinite coordination polymer fibers for carcinoembryonic antigen has been reported [57]. Hayat et al. developed a label-free novel amperometric immunosensor for the detection of okadaic acid [58]. Okadaic acid was also detected using amperometric immunosensor with great sensitivity in mussel sample via automated flow-through method [59]. In another study, a selective ultrasensitive detection of cortisol (metabolic regulator hormone) was developed after functionalizing gold nanowires and was aligned in such a way to work as an electrode in a three-electrode system [65]. Elsewhere, one of sub-unit was assembled using a gold support and the other on catalytic platinum NPs (PtNPs). Afterward, the creation of cocaine-aptamer complex was confirmed via PtNP-mediated reduction of hydrogen peroxide in the presence of cocaine. AuNP-grafted carbon nanotubes with various hydrazine labels have been utilized in an amplification approach to detect neomycin (an antibiotic) with a limit of detection (LOD) of 6.76 ± 0.17 ng/mL [66].

Ion channel switch
Ion channels offer high sensitivity in detection of biological molecules [67]. It is utilized for the quantitative detection of a wide range of target molecules, especially drug, proteins, toxins, and bacteria [68]. Ion channels are embedded in a tethered bilayer membrane and linked on the gold electrode, which generates an electric circuit. The capture biomolecules like antibodies are bound to the ion channel in such a way that the bonded target molecule controls the flow of ions through a channel. This produces a significant variation in the electrical conduction that is directly proportional to concentration. Biosensor based on ion channel can be formed using gramicidin (a chain of the dimmer peptide), inside the tethered bilayer membrane [69]. An antibody linked one of the peptides of the gramicidin is mobile, and other is static. The flow of ionic current stops through the membrane when dimer is broken. However, the extent of the variation in electrical signal is significantly amplified after separating of the membrane from the metal surface via a hydrophilic spacer.
Optical biosensors offer some great advantages as compared to conventional analytical methods since they enable real-time, label-free, and direct detection of numerous chemical and biological substances. Their advantages include sensitivity, high specificity, cost-effectiveness, and small size [83]. Optical biosensors can develop a cheap, multiplexed, and easily portable systems. Despite all these advantages, it can carry out label-free measurements. Newly fabricated nanostructures with size in nanometers have potential to direct light and can be utilized to examine different optical properties and phenomena of materials [84].

Microarrays
Microarrays are bi-dimensional receptor arrays, which offer simultaneous identification of several substances. Microarrays also have potential to detect small sized molecules, especially in biomedical. Peng et al. [85] prepared microarray to analyze three veterinary active molecules residues (i.e., tylosin, chloramphenicol, and clenbuterol. This microarray was synthesized using an indirect format, where small molecules are immobilized on the surface of a glass slide to compete with corresponding drug-antibodies. Subsequent the antigen-antibody linking, outcomes were measured via a secondary antibody labeled using Cy5. The detectability was attained in terms of IC50 for drugs; tylosin, chloramphenicol, and clenbuterol as 10.53 mg/L, 0.14 mg/L, and 0.53 mg/L, respectively.

Surface plasmon resonance
Surface plasmon resonance (SPR) can be defined as the oscillation (resonant) of conduction electrons over the interface between positive and negative permittivity material stimulated via incident light. SPR method has been developed and exploited as a powerful label-free between electrical field and free electrons present in a metal [86]. SPR waves circulate along the interface of a conducting layer, which is rich in free electrons and dielectrics. This method offers a simultaneous characterization of biomolecular interactions with high sensitivity using low quantity of sample. SPR technology has great application in protein-DNA interaction studies using biosensor-SPR approach [27].
Surface plasmons are widely used to increase the sensitivity of surface for several spectroscopic analyses including Raman scattering, second harmonic generation, and fluorescence ( Figure 1). SPR) is being exploited in different areas, especially in pharmaceuticals [60]. Recently, Fernandez et al. synthesized an SPR biosensor for identification of antibiotics in the milk using multiplexed analysis [87]. This multiplexed portable biosensor containing six channels were employed to analyze three antibiotics of different families viz., chloramphenicol, sulfonamides, and fluoroquinolones. The biosensor has been employed for detection of antibodies with wide-ranging spectrum. LODs values showed 1.1 µg/kg, 1.7 µg/kg, and 2.1 µg/kg for chloramphenicol, sulfapyridine, and enrofloxacin, respectively. These values highlight possibility of determining antibiotic residues lower the maximum residue limits.

Reagentless fluorescent (RF) biosensor
An RF biosensor facilitates detection of an analyte in the complex biological blend without using any other reagent. Hence, RF biosensor can work continuously when it is immobilized using a solid support. When a fluorescent biosensor interacts with an analyte, it results in an alteration in fluorescence properties. An RF biosensor is prepared after assimilating a biological receptor and a solvatochromic fluorophore. Fluorophore transduces the detection process into a quantifiable optical signal. Usually, an extrinsic fluorophore is widely utilized for which emission properties differ from intrinsic fluorophores of proteins such as tyrosine and tryptophan. This offer immediate detection and measuring of an analyte in the biological mixture. Both, antibodies and antigen binding proteins (AgBP) can be targeted directly against any kind of antigen, which makes them promise module for RF biosensors. When the structure of the antigen-complex is known, then this approach of integrating into AgBP of the solvatochromic fluorophore is used to convert into an RF biosensor [18]. Studies have suggested that fluorophore with the tendency of not forming a covalent bond on the surface of bioreceptor can produce RF biosensors of high quality owing to enhance in background signal due to interaction with the binding pocket over the antigen surface [88].

Glucose biosensors
The personal glucose meter (GM) is a typical device for point of care testing in household situations as of its comfort of use and consistent quantitative results. Recently, the DNA sensors, which were attached with a general GM were developed for the efficient detection of several targets, e.g., protein biomarkers [89,90] and recreational drugs [91,92].
Valentini et al. developed a glucose biosensor using gold microelectrodes coated via Single-Walled Carbon Nanotubes (SWCNTs), by the Electrophoresis Deposition Process. This nanostructured biosensor was successfully utilized to layer a poly (pyrrole)/glucose oxidase film. A highly extended linear concentration (ranging from 4 mM to 100 mM) of biosensor offered the opportunity to determine glucose level from 0.560 mM to 12.0 mM, with a high detection limit of 50 µM (useful for hypo-glycemia disease) [93]. A glucose biosensor working on the principle of direct transfer of an electron from glucose oxidase (GOD) and self-assembled over the surface of the electrochemically reduced carboxyl graphene (ERCGr) altered glassy carbon electrode has been prepared. X-ray photoelectron spectroscopy study of ERCGr showed that most of the oxygen-bearing groups in the carboxyl graphene, e.g., epoxy/ether and hydroxyl groups were removed excluding carboxylic acid. The cyclic voltammetric study of the electrode demonstrated a pair of quasi-reversible and welldefined redox peaks (-0.467 V) with a distance between peaks of 49 mV, which revealed that the electron transfer has occurred in between the electrode and GOD. The glucose biosensor exhibited a linear effect on the concentration of glucose in the range from 2 mM to 18 mM (detection limit=0.02 Mm) [94,95].

Applications
Biosensors exhibit several promising applications in the biomedical sciences ( Table 1). Some of the important applications are discussed in this review.

Glucose monitoring
Commercially available glucose monitoring is dependent on amperometric sensing of glucose via means of glucose oxidase that oxidizes glucose to produce hydrogen peroxide. Then, hydrogen peroxide is detected via the electrode. In order to overcome limitation owing to amperometric sensors. Several research articles are existing into novel sensing techniques, e.g., fluorescent glucose biosensors [115]. Palanisamy et al. studied successful recognition of the direct electrochemistry of GOD on modified electrode of silver NPs (RGO/Ag) nanocomposite and reduced graphene oxide [116]. In another study, Tang et al. demonstrated antigen-antibody interaction via GOD conjugated hollow gold-silver microspheres (AuAgHSs) further coupled using Prussian blue NPs (an artificial catalase), on a graphene-based immunosensor [117].

DNA biosensors
DNA biosensors (i.e., genosensors) are being utilized theoretically in different area viz., forensic science, medical diagnostics, agriculture, and in environmental clean-up. A significant advantage of DNA-based sensing devices is that it does not require any external monitoring. However, DNA biosensors are complex mini machines, which consist of micro lasers, sensing elements, and signal generator. In DNA, two strands are stick to each other via virtue of attraction due to chemical forces. When two strands match up at each nucleotide site, it produces a fluorescent signal (glow), which then transmitted to the signal generator. Genosensors have been employed for their intrinsic suitability and physicochemical stability to distinguish various organism strains. DNA hybridization occurs specifically on the surface of the physical transducer; this principle is used for genosensors based detection [118].

Microbial biosensors
Many microbial based biosensors (e.g., arsenic biosensor) have been developed by scientists using biological engineering. Bacteria are used for detection of pollutants in the samples. In order to detect arsenic, biologists utilize Ars operon [119].

Nanomaterials as biosensors
The nanomaterial hybrids developed after utilizing nanostructures (i.e., nanowires, nanoparticles, carbon nanotubes, nanorods, etc.) reveal combined characteristics of the specific nano materials [120]. A complex of silver and gold NPs have been developed to intensify the electrochemical signal in affinity-based sensors [121]. Other nanocomposites explored for immobilization study include Fe3O4chitosan, magnetic nanoparticles-silica, Pt/carbon nanotubes, and PANI/PVA-AgNP [122][123][124][125]. However, composite nanomaterials and their hybrid have shown great attraction as electrochemical biosensors. These hybrid constructions can act as signal amplifiers, immobilization supports, and probe for generating an electrochemical signal. For instance, Iron-silica complexes have been utilized for immobilization of biomolecule owing to their improved biocompatibility [126]. ZnO and Pt can be used in the preparation of several classes of nanomaterials to enhance the efficiency of amperometric biosensors [127]. Attaching of NPs to the QCM results into the large surface area, variability in shape, and physicochemical malleability. However, optional linking of metals empowers for different properties such as a) change in responses, b) specificity and c) selectivity. This transducer's features in a grouping with NPs increase surface area to volume ratios, which form it a perfect biosensor to primarily measure the malignancy and metastatic power of cancer cells. Atay Gold NPs and graphene Immobilization support [29] Platinum nanotubes modified with poly-amidoamine Amplification of signals [15] Enzymatic biosensor Carboxygraphene Enhance electrical properties [94] Graphene nanoplatelet-titanate nanotube composite Immobilization support [129] Graphene oxide and silver NPs Immobilization support [116] iron oxide-chitosan nanocomposite Immobilization support [130] Hydrogen per oxide sensor Graphene oxide (ERGO)-silver NPs Enzyme impersonators [131] MnO2 Enzyme impersonators [132] Immunosensor Ferroferric oxide NPs Amplification of signals [50] Gold NPs Amplification of signals [133] Gold NPs/polyaniline nanofibers Immobilization support [134] Graphene, platinum

Nanomaterials biosensors in cancer cell
In contrast to radiology imaging tests that direct some forms of energy (i.e., magnetic fields and X-rays, etc.) through the body to take interior images only. Biosensors can directly examine the malignant influence of the tumor. Both detector and biological elements utilize a small quantity of sample to test. Biosensors exist with compact design, rapid detection, rapid signals, high sensitivity, and high selectivity for the analyte. As compared to typical radiology imaging, biosensors have many benefits such as-a) it determines the extent of spread of cancer, b) checking of the effectiveness of treatment, c) prove more efficient (in cost, time, and productivity). Biological researchers have developed oncological biosensors for the treatment of breast cancer [137]. Another example of the biosensor is a transferrin quartz crystal microbalance (QCM). Atay et al. revealed selectivity and the specificity (in vitro) between QCM and MCF 7 cells, MDA-MB 231 breast cells, and starved MDA-MB 231 cells. They developed a technique of washing these various metastatic cells as compared to sensors to calculate mass shifts owing to different amounts of transferrin receptors. Results showed high selectivity for transferrin receptors since they are over-expressed in cancer cells. They revealed higher binding affinity to QCM [137].
Human epidermal growth factor receptor 2 (HER2 or ERBB2) is a significant biomarker found in breast cancer and was detected by Electrochemical impedance spectroscopy using a self-assembled monolayer of cysteine [138]. Lipoproteins [139], serum oncomarker CA125 [140], and tissue polypeptide antigen [141] were also sensitively detected via impedance, and impedimetric derived immunoassays in ovarian cancer patients for early diagnostics. In another study, Wu et al. confirmed an electrochemical visual platform for the efficient detection of cancer biomarkers by the integration of a closed bipolar electrode array and driving electrodes into a multichannel chip [142]. Shi et al. developed a bipolar electrode sensing platform, which coupled an electrochemiluminescence sensor with anodic dissolution for the sensitive detection of cancer cells [143]. In another study, the microfluidic immunoarray presented exceptional performance (within 36 min) in the instantaneous detection of the four types of prostate cancer protein biomarkers [144].

Food analysis
In the food industry, antibodies coated optics are generally utilized to detect food toxins and pathogens. Since fluorescence based optical and sulfonamides have been prepared to utilize SPR based sensor systems, often revised from current ELISA or from another immunological assay. The biosensor is an efficient, attractive, and alternative method to various other techniques. Since it is reliable and responds quickly. It showed high potential in the food industry for monitoring quality and safety of food as well as in bioprocessing industries [145].

Ozone biosensors
Ozone biosensors are important since ozone filters harmful ultraviolet radiation. The finding of the hole in the ozone layer has become a matter of worry. How much ultraviolet (UV) light reaches the surface of the earth and how deeply it reaches into sea water. Ultraviolet radiation can penetrate sea and can produce harmful effects on marine organisms, especially floating microorganisms (plankton). Plankton is the basis of marine food chains and is supposed to affect earth's weather and temperature via maintaining a balance between oxygen and CO 2 by photosynthesis. Karentz et al. have developed a simple technique for measuring intensity and UV penetration. A thin plastic bags was submerged to several depths holding particular strains of E. coli. The study revealed that E. coli were impotent to repair damage caused via ultraviolet radiation to their DNA. The bacterial "biosensors" showed persistent significant damage owing to UV light at depths of 10 m and regularly at 20 m and 30 m [146].

Discussion
The first biosensor was introduced in the 1960s and described the application of enzyme based bioelectrodes and their biocatalytic action. Afterward, several types of biosensors are being designed and utilized that include; cell or tissue-based, enzyme based, immunosensors, thermal and piezoelectric biosensors, and nucleic acid biosensors.
Enzyme based biosensors are being developed using immobilization techniques, i.e., covalent or ionic bonding and adsorption of enzymes via van der Waals forces by utilizing enzymes such as polyphenol oxidases, oxidoreductases, amino-oxidases, and peroxidases. Whereas, the tissues-based sensors were designed from animal and plant sources. In addition, the organelle-based sensors were developed by exploiting chloroplasts, membranes, microsomes, and mitochondria. Organelle-based biosensor reveals high stability but shows longer time for detection with decreased specificity. Antibodies based biosensors have more affinity towards particular antigens, viz., the antibodies bind specifically to the toxins or pathogens, or interact with different components of the immune system of the host. The nucleic acid biosensors are developed on the fact that single-strand of the nucleic acid molecule could recognize and attach to its complementary strand. The interaction occurs owing to the formation of hydrogen bonds between the nucleic acid strands.
Piezoelectric biosensors such as the surface acoustic wave device and the quartz crystal microbalance are based on the determination of deviations in resonance frequency in the piezoelectric crystal owing to weight changes in the structure of the crystal. First optical biosensor for commercial purpose was introduced in the late 1980s, since then a large number of optical biosensors has been described in research and development especially in diagnostic and pharmaceutical industries. These research include virology, epitope mapping, ligand fishing, cell biology cell adhesion, bacteriology, nucleotide-nucleotide binding, enzyme mechanisms, molecular engineering, nucleotide-protein, and signal transduction. Optical biosensors comprise of several optical components to produce a light beam having a specific property. SPR method has been developed and exploited as a powerful labelfree technique for the investigation of nucleic acid interactions. This method offers a simultaneous characterization of biomolecular interactions with high sensitivity using low quantity of sample. SPR technology has great application in protein-DNA interaction studies using biosensor-SPR approach. Surface plasmons are widely used to increase the sensitivity of surface for several spectroscopic analyses including Raman scattering, second harmonic generation, and fluorescence. The personal GM is a typical device for point of care testing in household situations as of its comfort of use and consistent quantitative results. Recently, the DNA sensors, which were attached with a general GM were developed for the efficient detection of several targets, e.g., protein biomarkers and recreational drugs.

Conclusion
Several newly developed techniques ranging from electrochemical, optical including fluorescence-based, and electromechanical are modern transducing methods, which are widely utilized in the development of biosensors. Different biosensors are now available in various fields viz., biomedicine, novel drug discovery, environmental monitoring, food safety processing, security, and defense. Thus, the invention of various powerful accurate and sensitive biosensors of immense importance e.g., electrochemical immunosensors, surface plasmon resonance sensor, glucose biosensors, nucleic acid biosensors and so on has offered many benefits over existing methods (i.e., improved sensitivity, less time, high-throughput screening, the possibility of developing label-free detection methods, and real-time analysis). Recent developments in biological techniques and instrumentation after utilizing fluorescence tag to various nano carriers namely; NPs, nanowires, nanotubes, etc. have improved the sensitivity of biosensors. Utilization of nucleotides or aptamers, affibodies, molecule imprinted polymers, and peptide arrays offer great tools to prepare advanced biosensors as compared to traditional techniques. Merging of nanotechnology with biosensor systems can enhance the diagnostic capability.