Open Access, Peer-reviewed
pISSN 2288-3886
eISSN 2288-3959
    J Nutr Health. 2014 Oct;47(5):313-320. Korean.
    Published online Oct 31, 2014.
    https://doi.org/10.4163/jnh.2014.47.5.313
    © 2014 The Korean Nutrition Society
    Original Article

    Hypoglycemic and antioxidant effects of jaceosidin in streptozotocin-induced diabetic mice

    Eunkyo Park,1 Byoung-Mog Kwon,2 In-Kyung Jung,3 and Jung-Hyun Kim3
      • 1Department of Home Economics, Graduate School, Chung-Ang University, Seoul 156-756, Korea.
      • 2Division of Biomedical Convergent, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea.
      • 3Department of Physical Education, Chung-Ang University, Seoul 156-756, Korea.
    • To whom correspondence should be addressed. tel: +82-02-820-5378, Email: jjhkim@cau.ac.kr
    Received July 24, 2014; Revised August 08, 2014; Accepted August 27, 2014.

    This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Abstract

    Purpose

    In this study, we investigated the effects of jaceosidin on blood glucose regulation in type 1 diabetic mice.

    Methods

    C57BL/6 mice were divided into four groups; normal control (Normal), diabetes control (D-Control), diabetes low-jaceosidin (D-0.005%), and diabetes high-jaceosidin (D-0.02%). Type 1 diabetes was induced by streptozotocin and mice were then fed a diet containing jaceosidin for eight weeks. Fasting blood glucose, oral glucose tolerance test, insulin tolerance test, lipid peroxidation, and antioxidant enzyme activities were assessed.

    Results

    Jaceosidin supplementation for eight weeks had no effect on body weight, organ weight, and blood lipid profiles. However, jaceosidin supplementation significantly lowered fasting blood glucose level and reduced insulin resistance. We also found that jaceosidin supplementation increased antioxidant capacity by enhancement of catalase and GSH-px activities.

    Conclusion

    These results suggest that jaceosidin could be a therapeutic candidate to ameliorate hyperglycemia through increase of antioxidant enzyme activity.

    Keywords
    jaceosidin; diabetes mellitus; antioxidant; lipid peroxidation

    Figures

    Fig. 1
    Effects of jaceosidin intake on fasting blood glucose, oral glucose tolerance test and insulin tolerance test in STZ-induced mice. A: Fasting blood glucose levels after 8 weeks of diet, B: Oral glucose tolerance test (OGTT) was performed at 6 week of diet. C: Area of under curve (AUC) was calculated based on OGTT results. D: Insulin tolerance test (ITT) was performed at 7 week of diet. E: AUC was calculated based on ITT results.

    Tables

    Table 1
    Composition of experimental diets (g/Kg diet)

    Table 2
    Effects of jaceosidin on changes in body weight, food intake, and food efficiency ratio in STZ-induced diabetic mice

    Table 3
    Effects of jaceosidin on organ weight in STZ-induced diabetic mice

    Table 4
    Effects of jaceosidin on liver function in STZ-induced diabetic mice

    Table 5
    Effects of jaceosidin on liver lipid peroxidation and antioxidant enzyme activities in STZ-induced diabetic mice

    Notes

    This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (NRF-2013R1A1A2006028).

    References

      1. Lim S, Lee EJ, Koo BK, Cho SI, Park KS, Jang HC, Kim SY, Lee HK. Increasing trends of metabolic syndrome in Korea-based on Korean National Health and Nutrition Examination Surveys. J Korean Diabetes Assoc 2005;29(5):432–439.
      1. Alberti KG, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998;15(7):539–553.
      1. Pambianco G, Costacou T, Ellis D, Becker DJ, Klein R, Orchard TJ. The 30-year natural history of type 1 diabetes complications: the Pittsburgh Epidemiology of Diabetes Complications Study experience. Diabetes 2006;55(5):1463–1469.
      1. Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes 1991;40(4):405–412.
      1. Eppens MC, Craig ME, Cusumano J, Hing S, Chan AK, Howard NJ, Silink M, Donaghue KC. Prevalence of diabetes complications in adolescents with type 2 compared with type 1 diabetes. Diabetes Care 2006;29(6):1300–1306.
      1. Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001;414(6865):813–820.
      1. Loomans CJ, de Koning EJ, Staal FJ, Rookmaaker MB, Verseyden C, de Boer HC, Verhaar MC, Braam B, Rabelink TJ, van Zonneveld AJ. Endothelial progenitor cell dysfunction: a novel concept in the pathogenesis of vascular complications of type 1 diabetes. Diabetes 2004;53(1):195–199.
      1. Giacco F, Brownlee M. Chapter 35. Pathogenesis of microvascular complications. In: Holt RI, Cockram C, Flyvbjerg A, Goldstein BJ, editors. Textbook of Diabetes. 4th edition. Chichester: Wiley-Blackwell; 2010. pp. 555.
      1. Jacob RA. The integrated antioxidant system. Nutr Res 1995;15(5):755–766.
      1. Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev 2002;23(5):599–622.
      1. Yasunari K, Maeda K, Nakamura M, Yoshikawa J. Oxidative stress in leukocytes is a possible link between blood pressure, blood glucose, and C-reacting protein. Hypertension 2002;39(3):777–780.
      1. Monnier L, Mas E, Ginet C, Michel F, Villon L, Cristol JP, Colette C. Activation of oxidative stress by acute glucose fluctuations compared with sustained chronic hyperglycemia in patients with type 2 diabetes. JAMA 2006;295(14):1681–1687.
      1. Baynes JW, Thorpe SR. Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes 1999;48(1):1–9.
      1. Irshad M, Chaudhuri PS. Oxidant-antioxidant system: role and significance in human body. Indian J Exp Biol 2002;40(11):1233–1239.
      1. Lee SD, Park HH, Kim DW, Bang BB. Bioactive constituents and utilities of Artemisia sp. as medical herb and foodstuff. Korean J Food Nutr 2000;13(5):490–505.
      1. Ryu SN, Kang SS, Kim JS, Ku BI. Quantitative analysis of eupatilin and Jaceosidin in Artemisia herba. Korean J Crop Sci 2004;49(6):452–456.
      1. Al-Mustafa AH, Al-Thunibat OY. Antioxidant activity of some Jordanian medicinal plants used traditionally for treatment of diabetes. Pak J Biol Sci 2008;11(3):351–358.
      1. Hill JO, Peters JC. Biomarkers and functional foods for obesity and diabetes. Br J Nutr 2002;88 Suppl 2:S213–S218.
      1. Min SW, Kim NJ, Baek NI, Kim DH. Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps on carrageenan-induced inflammation in mice. J Ethnopharmacol 2009;125(3):497–500.
      1. Riccardi G, Capaldo B, Vaccaro O. Functional foods in the management of obesity and type 2 diabetes. Curr Opin Clin Nutr Metab Care 2005;8(6):630–635.
      1. Kang YJ. In: Beneficial effects of eupatilin and jaceosidin isolated from Artemisia princeps on regulation of glucose, lipid and antioxidant metabolism in type 2 diabetic mice [dissertation]. Daegu: Kyungpook National University; 2008.
      1. Farhangkhoee H, Khan ZA, Mukherjee S, Cukiernik M, Barbin YP, Karmazyn M, Chakrabarti S. Heme oxygenase in diabetes-induced oxidative stress in the heart. J Mol Cell Cardiol 2003;35(12):1439–1448.
      1. Nagareddy PR, Xia Z, McNeill JH, MacLeod KM. Increased expression of iNOS is associated with endothelial dysfunction and impaired pressor responsiveness in streptozotocin-induced diabetes. Am J Physiol Heart Circ Physiol 2005;289(5):H2144–H2152.
      1. Komers R, Lindsley JN, Oyama TT, Schutzer WE, Reed JF, Mader SL, Anderson S. Immunohistochemical and functional correlations of renal cyclooxygenase-2 in experimental diabetes. J Clin Invest 2001;107(7):889–898.
      1. Huang X, Yuang J, Goddard A, Foulis A, James RF, Lernmark A, Pujol-Borrell R, Rabinovitch A, Somoza N, Stewart TA. Interferon expression in the pancreases of patients with type I diabetes. Diabetes 1995;44(6):658–664.
      1. Wentholt IM, Kulik W, Michels RP, Hoekstra JB, DeVries JH. Glucose fluctuations and activation of oxidative stress in patients with type 1 diabetes. Diabetologia 2008;51(1):183–190.
      1. Ruggenenti P, Remuzzi G. Primary prevention of renal failure in diabetic patients: the Bergamo Nephrologic Diabetes Complication Trial. J Hypertens Suppl 1998;16(1):S95–S97.
      1. Blake R, Trounce IA. Mitochondrial dysfunction and complications associated with diabetes. Biochim Biophys Acta 2014;1840(4):1404–1412.
      1. Yang HG, Kim HJ, Kim HS, Park SN. Antioxidative and antibacterial activities of Artemisia princeps Pampanini extracts. Korean J Microbiol Biotechnol 2012;40(3):250–260.
      1. Kim MJ, Kim DH, Lee KW, Yoon DY, Surh YJ. Jaceosidin induces apoptosis in ras-transformed human breast epithelial cells through generation of reactive oxygen species. Ann N Y Acad Sci 2007;1095:483–495.
      1. Lortz S, Tiedge M. Sequential inactivation of reactive oxygen species by combined overexpression of SOD isoforms and catalase in insulin-producing cells. Free Radic Biol Med 2003;34(6):683–688.
      1. Fu Z, Zhen W, Yuskavage J, Liu D. Epigallocatechin gallate delays the onset of type 1 diabetes in spontaneous non-obese diabetic mice. Br J Nutr 2011;105(8):1218–1225.
      1. Alam MM, Meerza D, Naseem I. Protective effect of quercetin on hyperglycemia, oxidative stress and DNA damage in alloxan induced type 2 diabetic mice. Life Sci 2014;109(1):8–14.
      1. Hong JH, Jeon JL, Lee JH, Lee IS. Antioxidative properties of Artemisia princeps Pamp. J Korean Soc Food Sci Nutr 2007;36(6):657–662.
      1. Lee SJ, Shin JH, Ju JC, Kang SK, Sung NJ. Hypoglycemic and hypolipidemic effects of Orostachys japonicus with medicinal herbs in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2013;42(4):587–594.
      1. Ramachandran V, Saravanan R. Asiatic acid prevents lipid peroxidation and improves antioxidant status in rats with streptozotocin-induced diabetes. J Funct Foods 2013;5(3):1077–1087.
      1. Kim MW. Effect of Sea Buckthorn leaves on hepatic enzyme levels in streptozotocin induced diabetic rats. J Korean Soc Food Sci Nutr 2013;42(1):40–45.
      1. Anaya-Eugenio GD, Rivero-Cruz I, Rivera-Chávez J, Mata R. Hypoglycemic properties of some preparations and compounds from Artemisia ludoviciana Nutt. J Ethnopharmacol 2014;155(1):416–425.
      1. Yuan H, Meng S, Wang G, Gong Z, Sun W, He G. Hypoglycemic effect of triterpenoid-rich extracts from Euryale ferox shell on normal and streptozotocin-diabetic mice. Pak J Pharm Sci 2014;27(4):859–864.
      1. Nakano M, Onodera A, Saito E, Tanabe M, Yajima K, Takahashi J, Nguyen VC. Effect of astaxanthin in combination with alpha-tocopherol or ascorbic acid against oxidative damage in diabetic ODS rats. J Nutr Sci Vitaminol (Tokyo) 2008;54(4):329–334.
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    MeSH Terms
    Animals
    Antioxidants*
    Blood Glucose
    Body Weight
    Catalase
    Diabetes Mellitus
    Diet
    Fasting
    Glucose Tolerance Test
    Hyperglycemia
    Insulin
    Insulin Resistance
    Lipid Peroxidation
    Mice*
    Organ Size
    Streptozocin
    Metrics
    Share
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