Okra cultivation under irrigation with saline water and foliar application of hydrogen peroxide

Foliar application of hydrogen peroxide can induce plant defense mechanisms against salt stress, favoring plant acclimation in regions with qualitative and quantitative scarcity of water resources, such as the Brazilian semi-arid region. From this perspective, this study aimed to evaluate the effects of foliar hydrogen peroxide application on chlorophyll a fluorescence, growth, and production of okra under irrigation with saline water. The experiment was conducted under field conditions in Pombal, PB, using a randomized block design with a 5 × 3 factorial arrangement corresponding to five electrical conductivity levels of water – ECw (0.3, 1.3, 2.3, 3.3, and 4.3 dS m -1 ) and three hydrogen peroxide concentrations – H 2 O 2 (0, 25, and 50 μM), with five replications. Irrigation water salinity levels up to 2.2 dS m -1 increase the maximum fluorescence of okra plants 75 days after transplanting. Foliar application of 50 µM hydrogen peroxide proved to be beneficial for plant height, stem diameter, stem dry matter, root dry matter, and total dry matter of okra when plants were grown in low-salinity water. The hydrogen peroxide concentrations of 25 and 50 µM increased the number of leaves. However, these concentrations reduced the average weight of the okra dry fruits. Foliar application with 50 µM hydrogen peroxide had a significant effect on the dry leaf phytomass of the okra cv. Clemson


INTRODUCTION
Okra (Abelmoschus esculentus (L.) Moench) is a crop of great socio economic importance for northeastern Brazil, being found in virtually all tropical and subtropical areas and standing out for its early vegetative cycle, high yield, low production costs, and high food and nutritional value.It is a vegetable source of fiber, vitamin A, C, B6, calcium, iron, magnesium, phosphorus, sodium and potassium (Couto and Coqueiro, 2020;Soares et al., 2020) Although okra plants are adapted to different soil conditions of the semi-arid region of Brazil (Modesto et al., 2019;Lima et al., 2020), this crop faces production limitations due to the qualitative and quantitative scarcity of water resources (Goes et al., 2020) as a result of long drought periods and high evapotranspiration rates (Lopes et al., 2020).
Most vegetables are glycophytic, showing changes in the photosynthetic process and allocation of photoassimilates due to osmotic and ionic effects, with impacts on plant growth and production (Oliveira et al., 2017;Alvarenga et al., 2019).Okra plants, in turn, have a threshold salinity of 1.3 dS m -1 in the saturation extract (Campos, 2013;Gheyi et al., 2016), but can be cultivated under higher salinity levels as long as in association with substances that mitigate the deleterious effects of salt stress in plants (Zafar et al., 2021;Mendonça et al., 2022a;Silva et al., 2023).Although there are several strategies in the literature that aim to mitigate the effects of saline stress on okra plants, the intensity of the deleterious effects on the plants depends on the culture, cultivar, development phase, irrigation and fertilization management and climatic conditions (Pinheiro et al., 2022;Mendonça et al., 2022a) In this scenario, it is essential to develop strategies that mitigate the effects of salt stress in plants.The available alternatives include foliar application of hydrogen peroxide (H2O2) (Veloso et al., 2023), which can act as a signaling molecule in plants under biotic and abiotic stresses and induce defense mechanisms before exposure to stress (Mendonça et al., 2022b).
H2O2 can favor the accumulation of proteins and soluble carbohydrates that act as organic solutes and contribute to the osmotic adjustment of plants under salt stress (Ramos et al., 2021;Veloso et al., 2023).Furthermore, it can act in cells as a secondary messenger, increasing the flow of Ca 2+ ions and modifying the pattern of proteins and gene expression (Bienert et al., 2006).Research carried out using foliar H2O2 application has highlighted beneficial effects in plants under salt stress conditions, as observed in pepper (Aragão et al., 2023), soursop (Silva et al., 2019a), sour passion fruit (Silva et al., 2019b), and colored fiber cotton (Veloso et al., 2023).
The hypothesis of this study is that the foliar application of hydrogen peroxide in low concentrations works as an abiotic stress signaling molecule, playing an important role in the growth and production of okra plants under saline stress.From this perspective, this study aimed to evaluate the effects of foliar hydrogen peroxide application on chlorophyll a fluorescence, growth, and production of okra under irrigation with saline water.

MATERIAL AND METHODS
The experiment was conducted under field conditions from December 2020 to March 2021, at the Agri-food Science and Technology Center -CCTA of the Federal University of Campina Grande -UFCG, Pombal, Paraíba, located at the geographic coordinates 6º46'13" S, 37°48'06" W, and at an elevation of 193 m a.s.l.
A randomized block design with a 5×3 factorial arrangement was adopted, corresponding to five water electrical conductivity levels - ECw (0.3,1.3,2.3,3.3,and 4.3 dS m -1 ) and three hydrogen peroxide concentrations -H2O2 (0, 25, and 50 μM), with five replications and one plant per plot.The water salinity levels were based on research carried out by Soares et al. (2020).The concentrations of H2O2 used in this study were based on previous research carried out with summer squash (Dantas et al., 2021).
Sowing was carried out in a 50-mL polyethylene tray using the cultivar Clemson Americano 80, with two seeds per cell in a substrate obtained by mixing soil, sand, and cattle manure in a ratio of 2:1:1 (on a volume basis), respectively.At this stage, the plants were irrigated daily with low-salinity water (0.3 dS m -1 ) and subsequently thinned to one plant per cell.
Transplanting was carried out 20 days after sowing (DAS) into 20-mL pots adapted as drainage lysimeters filled with a 0.5-kg-cm layer of gravel over a geotextile fabric covering the bottom of the container to avoid clogging.Soon after, the pots received 22 kg of a Fluvic Neosol with a sandy loam texture.A 15-mm diameter hose was also installed as a drain at the base of each lysimeter, coupled to a plastic container (2 L) to collect the drained water and determine water consumption by the plants.The chemical and physical characteristics of the soil used in the experiment (Table 1) were determined according to Teixeira et al. (2017).
Fertilization was carried out according to the recommendation of Novais et al. (1991) by applying 100 mg N kg -1 , 300 mg P2O5 kg -1 , and 150 mg K2O kg -1 using urea, monoammonium phosphate, and potassium chloride as the respective sources of nitrogen, phosphorus, and potassium.Fertilization was carried out via topdressing in three applications, starting 10 days after transplanting.Micronutrients were supplied every 15 days, and their application began 20 days after transplanting with the commercial product Micro Rexene® (Mg -1.2%, B -0.85%, Zn -4.2%, Fe -3.4%, Mn -3.2%, Cu -0.5%, and Mo -0.06%) at the concentration of 0.5 g L -1 .The different water electrical conductivity levels (ECw) were prepared by dissolving sodium chloride (NaCl) in local water supply (ECw = 0.3 dS m -1 ) while considering the relationship between the ECw and salt concentration (Richards, 1954), according to Equation 1: Where: C = salt concentration to be applied (mmolc L -1 ); ECw = water electrical conductivity (dS m -1 ).
At the time of transplanting, the soil moisture content was raised to the level corresponding to the maximum water retention capacity, and irrigation was carried out daily with water of low electrical conductivity (0.3 dS m -1 ) until 17 DAT.After this period, irrigation was started daily with different salinity levels.The water depth applied was determined based on drainage lysimetry in order to replace the average daily consumption (Equation 2). Where:

VC -volume consumed (L);
VAwater volume applied on the previous day; VDdrained volume, quantified in the morning of the next day; LFleaching fraction estimated at 0.1, every 10 days, in order to minimize salt accumulation in the root zone.
During the experiment, chemical interventions were carried out with preventive applications of commercial pesticides, e.g., insecticides and fungicides, using a manual compression sprayer for phytosanitary control.The different concentrations of H2O2 were prepared from a stock solution of hydrogen peroxide, obtained by diluting H2O2 -30% in deionized water in each application event.Applications were started 72 hours before irrigation with saline water (15 DAT), according to their respective treatments, using a manual sprayer.The product was applied on the abaxial and adaxial sides in order to completely wet the leaves.A cardboard structure was used to avoid drifting onto neighboring plants.During the experiment period, 2 mL of H2O2 was applied per plant.
Growth was determined at 75 DAT by determining the number of leaves (NL), considering those with a minimum length of 3 cm; plant height (PH) was measured from the base of the plant to the insertion of the apical meristem; stem diameter (SD) was determined at 2 cm from the ground with a digital caliper.Leaf area (LA) was measured by following the methodology of Fideles Filho et al. (2010), according to Equation 3: (3) Where: LAleaf area per plant (cm 2 ); X -length of the midrib of the respective leaf (cm).
Fruit harvest began at 59 DAT and continued until 82 DAT by removing fruits that had the typical green color of ripe fruits.The average fruit length (AFL) measured from the base to the apex of the fruit and the average fruit diameter (AFD) were evaluated using a digital caliper.Subsequently, the fruits were dried to constant weight in an air circulation oven at 65ºC, after which they were taken to a precision balance (0.01g), where the average dry fruit weight (ADF, g per plant) and total dry fruit weight (TDF, g per plant) were determined.
At 82 DAT, the plants were collected and separated into different parts (leaves, stems, and roots); subsequently, the parts were packed in paper bags and taken to an air circulation oven at 65ºC until reaching constant weight.After this period, the material was weighed on a semianalytical balance, obtaining the phytomass of leaves (DPL, g per plant), stem (DPS, g per plant), and roots (DPR, g per plant), the sum of which resulted in the total dry phytomass (TDP, g per plant).
The cumulative water consumption (WC, L per plant) was calculated from the sum of daily water consumption per experimental unit, considering the volume applied and drained during the experiment.
The data obtained were subjected to analysis of variance (F-test) at a probability level of 0.05.In cases of significance, linear and quadratic polynomial regression were performed for the water electrical conductivity levels and H2O2 concentrations using the statistical software SISVAR (Ferreira, 2019).In cases of significant interaction between factors, response surface graphs were created using the software SigmaPlot Version 12.5.

RESULTS AND DISCUSSION
There was a significant effect of water salinity on the maximum (Fm) and variable (Fv) fluorescence of okra plants (Table 2).Hydrogen peroxide concentrations and the interaction between factors (SL × H2O2) did not significantly influence the chlorophyll a fluorescence of  The maximum fluorescence of okra plants (Figure 1A) achieved the maximum estimated value under the ECw of 2.2 dS m -1 (2052.24),decreasing from this level.The lowest value occurred in plants grown under the ECw of 4.3 dS m -1 (1997.89).The increase in Fm is related to the reduction of the damage caused by salt stress, increasing the plant's capacity to transfer energy for the formation of NADPH, ATP, and reduce ferredoxin, in addition to favoring the assimilation of CO2 in the biochemical phase of photosynthesis (Figueiredo et al., 2019).The variable fluorescence (Fv) of okra plants also showed a quadratic behavior (Figure 1B), with the maximum estimated value of 1593.68 being achieved at the ECw of 1.9 dS m -1 .On the other hand, the minimum value of 1518.11 was achieved in plants subjected to irrigation with 4.3 dS m -1 (Figure 1B).Under salt stress conditions, deficiency in the photoreduction of quinone A (QA) and in the flow of electrons between photosystems may occur, probably due to the damage caused to the photosynthetic apparatus as a result of water stress caused by the osmotic effect, with the imbibition forces of plant roots becoming low in relation to the water retention forces in the soil due to excess salts (Cova et al., 2021).
Okra cultivation under irrigation with saline water … Rev. Ambient.Água vol.19, e2980 -Taubaté 2024 There was a significant effect of the interaction between factors (SL × H2O2) on plant height and okra stem diameter at 75 DAT.Water salinity levels significantly influenced all growth variables measured (Table 3).Hydrogen peroxide concentrations had a significant effect only on the number of leaves and leaf area of okra.The plant height (Figure 2A) of okra reached the maximum estimated value under the ECw of 0.3 dS m -1 (77.92 cm) and foliar application of 50 µM of H2O2, being 27.6 cm higher than the lowest value (50.32 cm) obtained under irrigation with 4.3 dS m -1 and at a concentration of 0 µM of H2O2.When the plants received irrigation with 1.8 dS m -1 and 50 µM of H2O2, this decrease was only 13.57 cm in relation to the highest value found.The beneficial effects of hydrogen peroxide may be related to its role as an abiotic stress signal in plants, activating the defense system and favoring adaptation to adverse conditions (Souza et al., 2019).Irrigation with 0.3 dS m -1 and foliar hydrogen peroxide application at a concentration of 50 µM resulted in the highest value (18.91 mm) of stem diameter (SD) in okra plants (Figure 2B).When the plants received irrigation with an ECw of 4.3 dS m -1 and a concentration of 12.5 µM of H2O2, they showed a SD of 15.24 mm, representing a reduction of 19.41% compared to the highest estimated value.The foliar application of hydrogen peroxide at adequate concentrations can favor greater accumulation of proteins and soluble carbohydrates that act in the osmotic adjustment of plants under salt stress, thus allowing greater water and nutrient uptake and, consequently, greater plant growth (Aragão et al., 2023).
The number of leaves (NL) decreased linearly with the increase in the water electrical conductivity levels (Figure 3A), with reductions of 11.82% per unit increase in the ECw.When comparing the NL of plants subjected to an ECw of 4.3 dS m -1 to those irrigated with the lowest salinity, a reduction of 50.9% was observed.Reductions in the number of leaves in plants subjected to salt stress are considered a protection and/or acclimatization strategy to high salinity, minimizing water losses through transpiration and maintaining a high water potential in the cell (Nascimento et al., 2019).The leaf area (LA) of okra plants was also negatively affected by water salinity (Figure 3B), with the maximum estimated value of LA (2738.39cm 2 ) obtained in plants grown under the ECw of 1.3 dS m -1 , decreasing from this point onwards and reaching the minimum value of 1802.28 cm 2 under the ECw of 4.3 dS m -1 .The leaf area reduction constitutes one of the initial responses of plants to salt stress and may be related to cellular inhibition and leaf surface expansion (Lima et al., 2017).Under these conditions, plants delay the emission of leaves (Figure 4A) and deactivate part of their leaf area through leaf abscission, in an attempt to reduce water loss through transpiration (Dias et al., 2017).
Hydrogen peroxide (H2O2) at a concentration of 50 µM (Figure 3C) resulted in a number of leaves (NL) 16.43% higher than plants grown in the absence of H2O2 (0 µM).However, Rev. Ambient.Água vol.19, e2980 -Taubaté 2024 when comparing the NL of plants that received foliar application of 25 and 50 µM of H2O2, it appears that there was no significant difference between them.The application of H2O2 favors the activation of physiological responses that allow the accumulation of proteins, soluble carbohydrates, and NO3 -, inducing cell division and increasing the emission of leaves (Gondim et al., 2011).
There was a significant effect of the interaction between salinity levels and hydrogen peroxide (SL × H2O2) on the stem (DPS), root (DPR) and total (TPM) dry matter of okra plants (Table 4) 82 days after transplanting.The water salinity levels significantly affected all variables analyzed.The hydrogen peroxide concentrations significantly influenced the DPL, DPR, and TDP of okra plants at 82 DAT.The dry phytomass of okra leaves (DPL) was negatively affected by the increase in water electrical conductivity (Figure 4A), with a reduction of 11.98% per unit increase in the ECw.A reduction of 49.72 % (14.27 g per plant) was observed when comparing the DPL of plants subjected to the ECw of 4.3 dS m -1 to those under the lowest electrical conductivity (0.3 dS m -1 ).The reduction in DPL is related to the lower emission of leaves observed in this study under salinity conditions as excess salts reduce the free energy status of water in the root environment and the availability of water and nutrients to plants and compromise meristematic activity and cell elongation, consequently restricting phytomass accumulation in the leaves (Shankar et al., 2016;Silva et al., 2020).
When analyzing the effects of Hydrogen peroxide (H2O2) concentrations on okra leaf dry matter (DPL) (Figure 4B), it was observed that plants subjected to foliar application of 50 µM of H2O2 had an NL 8.51 and 13.28% higher than those under 0 and 25µM of H2O2, respectively.These results are related to the effect of the highest H2O2 level (50 µM) on leaf emission, indicating the role of hydrogen peroxide in inducing the production of several enzymes, e.g., catalase, superoxide dismutase, glutathione reductase, and peroxidase, which are associated with plant detoxification (Das and Roychoudhury, 2014).Pereira et al. (2022) evaluated the effects of irrigation with saline water (ECw varying from 0.3 and 5.0 dS m -1 ) and four concentrations of H2O2 (0, 5, 10, and 15 µmol L -1 ) on melon and found that the highest dry phytomass values of the shoot part were 78.62 and 46.34 g per plant under ECw values of 0.3 and 5.0 dS m -1 and foliar application of 9.11 and 8.35 µmol L -1 of H2O2.Irrigation with low salinity water (ECw of 0.3 dS m -1 ) and foliar application of H2O2 at a concentration of 50 µM provided greater accumulation of dry phytomass in the stem (DPS) (28.71 g per plant).On the other hand, the water salinity of 4.3 dS m -1 and the concentration of 18.75 µM of H2O2 resulted in a lower DPS (10.54 g per plant), i.e., a reduction of 34.69% compared the plants grown under the ECw of 0.3 dS m -1 and 50 µM H2O2 (Figure 5A).For Bienert et al. (2006), hydrogen peroxide can act as a secondary messenger in cells by increasing the flow of Ca 2+ ions and modifying the pattern of proteins and gene expression.Andrade et al. (2019) evaluated four concentrations of hydrogen peroxide (0, 20, 40, and 60 µM) in sour passion fruit under salt stress and observed beneficial effects with foliar application of 20 µM of H2O2.For root dry phytomass (DPR), the maximum estimated value (31.02 g per plant) was obtained in plants grown under the ECw of 0.3 dS m -1 and foliar application of 50 µM of H2O2.On the other hand, the minimum value of 4.49 g per plant was reached in plants grown under the water salinity of 4.3 dS m -1 and foliar application of 0 µM H2O2 (Figure 5B).The restriction in water uptake due to the osmotic effect causes stomatal closure, reducing carbon accumulation in the cell and resulting in less photosynthesis, consequently decreasing phytomass accumulation (Silva et al., 2023).
The total dry mass (TDP) of okra plants achieved the maximum value (90.54 g per plant) under irrigation with 0.3 dS m -1 and foliar application of 50 µM of H2O2.The lowest value (30.31 g per plant) was achieved in plants irrigated with water at 4.3 dS m -1 and hydrogen peroxide at a concentration of 6.25 µM (Figure 5C).The toxicity of specific ions (Na + , Cl -, and B) occurs due to the absorption of saline water by plant roots, causing cell disruption and metabolic changes in plants and reducing growth and phytomass (Ali et al., 2021).
There was a significant effect of the interaction between factors (SL × H2O2) only for the total dry fruit weight (TDF) (Table 5).The salinity levels significantly influenced water consumption (WC).On the other hand, the hydrogen peroxide concentrations significantly affected the average dry fruit weight (TDF) of okra plants.The total weight of the dry fruit (TDF) decreased with increasing salinity levels (Figure 6), with the maximum value (18.39 g per plant) being achieved in plants irrigated with 0.3 dS m -1 and no H2O2 (0 µM).It should be noted that the increase in salinity levels associated with the increase in H2O2 concentrations intensified the deleterious effects of salt stress on okra, with irrigation with 4.3 dS m -1 and a concentration of 50 µM of H2O2 resulting in a reduction of 66.82% compared to the plants grown under the ECw of 0.3 dS m -1 .Azeem et al. (2017) explain that a reduction in photosynthetic efficiency in okra plants, which are sensitive to the effects of saline stress, decreases fruit growth and biomass production.Similar results were found by Soares et al. (2020) in research with okra and irrigation with saline water (ECw ranging from 0.3 to 4.3 dS m -1 ), in which they found that higher salinity levels reduce fruit weight, and the highest value of 33 .64g for average fruit weight was achieved in plants irrigated with an ECw of 2.4 dS m -1 and under hydroponic conditions (nutrient solution EC levels of 2.1, 3.6, 5.1, and 6.6 dS m −1 ), Mendonça et al. (2022a) observed a 3.82% reduction in the average weight of okra fruits per unit increase in the EC of the nutrient solution.With regard to the effects of H2O2 on the average dry fruit weight (Figure 7A), the foliar application of 25 and 50 µM H2O2 reduced this parameter by, respectively, 16.79 and 12.94% in plants that received 0 µM H2O2.The average dry fruit weight decreased with the application of H2O2, probably because it is a reactive oxygen species (ROS) which, depending on its concentration, can act in the oxidation of the lipid membrane, DNA, and other cell components through toxic free radicals (Barbosa et al., 2014;Silva et al., 2019c).The water consumption of okra (WC) plants decreased quadratically with the increase in the electrical conductivity of irrigation water (Figure 7B).Okra plants irrigated with an ECw of 0.3 dS m -1 achieved the highest WC (29.71 L per plant), decreasing sharply from this salinity Okra cultivation under irrigation with saline water … Rev. Ambient.Água vol.19, e2980 -Taubaté 2024

Figure 1 .
Figure 1.Maximum fluorescence -Fm (A) and variable fluorescence -Fv (B) of the okra cv.Clemson Americano 80 as a function of irrigation water salinity -ECw 75 days after transplanting.

Figure 2 .
Figure 2. Plant height (A) and stem diameter (B) of the okra cv.Clemson Americano 80 as a function of the interaction between irrigation water salinity -ECw and hydrogen peroxide concentrations -H2O2, 75 days after transplanting.X and Y -Electrical conductivity of irrigation water -ECw and hydrogen peroxide concentrations -H2O2, respectively; ns, *, **, non-significant and significant at p ≤ 0.05 and 0.01 by the F-test.

Figure 3 .
Figure 3. Number of leaves (A) and leaf area (B) of the okra cv.Clemson Americano 80 as a function of irrigation water salinity-ECw and number of leaves as a function of hydrogen peroxide concentrations -H2O2 75 days after transplanting.Equal letters indicate that the H2O2 means do not differ by the Tukey test at p<0.05.The bars represent the standard error of the mean (n = 5).

Figure 4 .
Figure 4. Dry phytomass of leaves of the okra cv.Clemson Americano 80 as a function of irrigation water salinity -ECw (A) and hydrogen peroxide concentrations -H2O2 (B) 82 days after transplanting.Equal letters indicate that the H2O2 means do not differ by the Tukey test at p<0.05.The bars represent the standard error of the mean (n = 5).

Figure 5 .
Figure 5. Dry phytomass of stem (A), roots (B) and total dry phytomass (C) of the okra cv.Clemson Americano 80 as a function of the interaction between irrigation water salinity -ECw and hydrogen peroxide concentrations -H2O2 82 days after transplanting.X and Y -Electrical conductivity of irrigation water-ECw and hydrogen peroxide concentrations -H2O2, respectively; ns , * , ** significant at p ≤ 0.05 and 0.01 by the F-test.

Figure 6 .
Figure 6.Total dry fruit weight (TDF) of the okra cv.Clemson Americano 80 as a function of the interaction between salinity levels of irrigation water -ECw and concentrations of hydrogen peroxide -H2O2 82 days after transplanting.X and Y -Electrical conductivity of irrigation water-ECw and hydrogen peroxide concentrations -H2O2, respectively; ns,*, **, non-significant and significant at p ≤ 0.05 and 0.01 by the F-test.

Figure 7 .
Figure 7. Average dry fruit weight -ADF of the okra cv.Clemson Americano 80 as a function of hydrogen peroxide concentrations, (A) and water consumption -WC of okra plants as a function of irrigation water salinity -ECw (B) 82 days after transplanting.Equal letters indicate that the H2O2 means do not differ by the Tukey test at p≤0.05.The bars represent the standard error of the mean (n = 5).

Table 1 .
Chemical and physical characteristics of the soil used in the experiment before the application of treatments.

Table 2 .
Summary of the analysis of variance for initial fluorescence (F0), maximum fluorescence (Fm), variable fluorescence (Fv) and quantum efficiency of photosystem II (Fv/Fm) of okra cv.Clemson Americano 80 cultivated with saline water and concentrations of hydrogen peroxide 75 days after transplanting (DAT).

Table 3 .
Summary of the analysis of variance for plant height (PH), stem diameter (SD), number of leaves (NL), and leaf area (LA) of the okra cv.Clemson Americano 80 cultivated with saline water and hydrogen peroxide concentrations 75 days after transplanting (DAT).

Table 4 .
Summary of the analysis of variance for dry phytomass of leaves (DPL), stem (DPS), roots (DPR) and total dry phytomass (TDP) of the okra cv.Clemson Americano 80 cultivated with saline water and hydrogen peroxide concentrations 82 days after transplanting (DAT).

Table 5 .
Summary of the analysis of variance for total dry fruit weight (TDF), average dry fruit weight (ADF), water consumption (WC), average fruit diameter (AFD), and average fruit length (AFL) of the okra cv.Clemson Americano 80 cultivated with saline water and hydrogen peroxide concentrations 82 days after transplanting (DAT).