Comparative Study of Nigella sativa and Triple Therapy in Eradication of Helicobacter pylori in Patients with Non-Ulcer Dyspepsia

A large number of diseases are ascribed to Helicobacter pylori (H. pylori), particularly chronic active gastritis, peptic ulcer disease and gastric cancer. Successful treatment of H. pylori infection with antimicrobial agents can lead to regression of H. pylori–associated disorders. Antibiotic resistance against H. pylori is increasing, and it is necessary to find new effective agents. Nigella sativa seed (NS), a commonly used herb, possesses in vitro anti-helicobacter activity. The present study was undertaken to evaluate the efficacy of NS in eradication of H. pylori infection in non-ulcer dyspeptic patients.

Helicobacter pylori (H.pylori) infection is extremely common worldwide, and a large number of diseases have been ascribed to H. pylori, particularly chronic active gastritis, peptic ulcer, gastric cancer and gastric Mucosa-Associated Lymphoid Tissue (MALT)-lymphoma. [1,2]Considering peptic ulcer alone, at least 6,000,000 new cases occur each year worldwide mainly linked to H. pylori.Figures regarding adenocarcinoma amount to 899,000 new cases annually, and 75% of these are probably linked to H. pylori infection. [3] pylori is a gram-negative flagellated spiral bacterium which is usually acquired during childhood, and the infection persists throughout life unless specifically treated.[4] Eradication of H. pylori infection is recommended to prevent ulcer recurrence and complications in all patients with documented peptic ulcer disease.[5] Since H. pylori has a peculiar habitat and characteristics, it is difficult to eradicate eradicate with a single antibiotic, [6] which is why the standard therapy includes a combination of at least two antibiotics along with a proton pump inhibitor (PPI).Nevertheless, current treatment regimens, including PPIs plus two antibiotics (usually clarithromycin and amoxicillin), fail to eradicate H. pylori in approximately 20% of the patients.[6] Recently, H. pylori has been found to be resistant to one or more of the antimicrobial drugs. [7]or example, in one of the studies the resistance was reported in 44% of cases to metronidazole and in 14% of cases to clarithromycin, [8] while in another study the resistance against the same drugs was 49.4% and 10.8%, respectively.[9] In light of this emerging resistance, there is a need to look for new remedies effective against H. pylori.The traditional use and anecdotal evidence of plants as medicine provide the basis for suggesting that plant extracts may be useful for specific medical conditions.It has been shown that essential oils, extracted from plants, are bactericidal against H. pylori without the development of acquired resistance, suggesting that essential oils may have potential as new and safe agents for inclusion in anti-H. Nigella sativa (N.sativa), one of the members of Ranunculaceae family, commonly grows in the Middle East, eastern Europe and eastern and middle Asia.N. sativa and its oil are being used as food additives as well as natural remedies for many ailments for over thousands of years.[11] Many active ingredients have been isolated from N. sativa, including: thymoquinone, thymohydroquinone, dithymoquinone, thymol, carvacrol, nigellicine and alphahedrin.[12] In addition, many pharmacological effects of N. sativa and its active principles have been identified, such as immune stimulation, anti-inflammatory, anti-cancer and antimicrobial activity.[12,13] The antibacterial effect of the phenolic fraction of N. sativa oil was first reported by Topozada in 1965. Recently, crude extracts of N. sativa were reported to have a promising effect on multi-drug-resistant organisms, including gram-positive and gram-negative bacteria.[16] In a recent in vitro study, N. sativa extract produced within 60 minutes, a 100% inhibition of the growth of all the strains of H. pylori that were tested.[17] Thus the present study aimed at the investigation of the effectiveness of N. sativa in eradication of H. pylori in nonulcer dyspeptic patients compared to that of standard triple therapy.

MATERIALS AND METHODS
The study was conducted in the gastroenterology endoscopy unit at King Fahd Hospital of the University (KFHU), Al-Khobar, Saudi Arabia, from March 2007 to August 2008.A total of 308 patients were initially enrolled in the study, out of which 110 were included according to the inclusion/ exclusion criteria.Of these 110 patients randomly assigned, 22 were excluded, discontinued or lost.All patients (n = 88; 32 male and 56 female; age range, 18-65 years) finally included in the study had complaints of dyspeptic symptoms and had positive result for H. pylori infection by both histopathology and rapid urease test Compylobacter-Like Organism (CLO) test.Patients were excluded if 1) the endoscopy showed peptic ulcer, gastric cancer or gastrointestinal bleeding; 2) they had taken proton-pump inhibitors, bismuth or antibiotics in the last four weeks before endoscopy; 3) they were pregnant or lactating mothers; 4) they were intolerant or allergic to therapeutic regimens; or 5) they failed to report for follow-up.The nature, aim and expected outcome of the study were explained to each patient, and written consent was obtained.The prospective study was approved by the medical and ethical committee of King Fahd Hospital of the University, Al-Khobar, Saudi Arabia, and conducted according to the guidelines of Helsinki declaration.
The trial profile is shown in Figure 1.The study was designed as an open, randomized clinical trial with four groups.The patients were randomly assigned to one of the four groups using alternate subject method as follows: group I: patients received triple therapy, i.e., clarithromycin tablets 500 mg twice daily and amoxicillin 1 g twice daily for one week; group II: patients received 1 g N. sativa powder as capsule of 500 mg (1 capsule twice daily after meals) for four weeks; group III: patients received 2 g N. sativa powder as capsule of 500 mg (2 capsules twice daily after meals) for four weeks; group IV: patients received 3 g N. sativa powder as capsule of 500 mg (2 capsules thrice daily after meals) for four weeks.Patients in all the groups received omeprazole 40 mg once daily for four weeks.Patients were instructed to avoid anti-ulcer and ulcerogenic medications and any antibiotic other than the drugs given until completion of study.They were advised to take normal balanced diet during treatment.
N. sativa capsules were obtained from BioExtract (Pvt.) Ltd., Sri Lanka, and each capsule contained 500 mg of ground N. sativa L seeds.Clarithromycin (Klacid ® , Abott, England) 500 mg capsules, amoxicillin (Amoxil ® , Beecham, England) 500 mg capsules and omeprazole (Gasec ® , Mepha, Switzerland) 20 mg capsules were obtained from a local pharmacy.The doses selected for the study were based on studies being carried out in children and adults. [18,19]The doses of 1, 2 and 3 g/d were considered safe in light of the acute and chronic toxicity studies of N. sativa fixed oil and of thymoquinone (the active ingredient in N. sativa) in mice. [20,21]ophago-gastroduodenoscopy (EGD) was done for every patient according to the standard protocol of Gastroenterology Division at KFHU.Mucosal states of esophagus, stomach and duodenum were assessed and recorded, and mucosal biopsies were taken from the antrum and the body of the stomach.Gastritis, duodenitis were diagnosed endoscopically based on the presence of erythema, erosion and nodularity, which was later confirmed by histology.Endoscopic diagnosis of gastroesophageal reflux disease (GERD) was made according to Los Angeles (LA) classification.Mucosal biopsy specimens were placed in fixative and sent for histopathological study, while one specimen was put in rapid urease test slide (CLO test -Kimberly-Clark-Ballard Medical Products, Draper, Utah, USA) for immediate detection of H. pylori.The presence of H. pylori was defined by two positive results (histopathologic examination and rapid urease test). [22]Patients were subsequently excluded from the study if the presence of H.The Saudi Journal of Gastroenterology pylori determined by rapid urease test was not confirmed by histopathological findings.
A rapid monoclonal immunochromatographic method (ImmunoCard STAT HpSA, Meridian Bioscience, Europe) was used to detect the presence of H. pylori antigen in the stool of the patients 4 weeks after the end of treatment. [23]he test was performed according to the instructions of the manufacturers.
Eligible patients were interviewed and they underwent physical examination, including weight and height.A questionnaire regarding demographic data (name, age, sex, etc.), as well as about the presence of other illnesses or habits (smoking, alcohol, etc.), was filled.The details of relevant gastrointestinal symptoms (epigastric pain and reflux symptoms) were filled for each patient, using a special scoring system that is routinely employed in the gastroenterology unit of the hospital and is a simplified adaptation of "Short-form Leeds Dyspepsia Questionnaire". [24]is system evaluated three important parameters regarding every dyspeptic symptom, viz., the frequency of the that particular dyspeptic symptom (0= less than 3 times/week; 1= most days of the week), relief of the symptom (0= spontaneously relieved; 1= relieved only by medication), and effect of that particular symptom on the quality of life (0= no effect, 1= presence of effect).The effect on quality of life was assessed as to whether the symptom interferes with daily routine working of the person or not.Scores for each of the symptoms could range from zero (no symptom) to a maximum of 3 (severe symptom).In addition, the patients were followed up weekly during the four weeks of treatment with N. sativa, through telephone contact.The followup information regarding improvement of the dyspeptic symptoms, appearance of side effects and compliance of the patients was documented in the hospital file of each patient.
No antibiotic or proton pump inhibitors were allowed during the two weeks preceding the stool antigen test.Finally at the end of the treatment, assessment of dyspeptic symptoms for each patient was done but endoscopy was not performed.The primary outcome measure was eradication of H. pylori, which was considered to be achieved on the basis of a negative stool antigen test four weeks after the end of treatment.The secondary outcome measure included changes in the clinical condition as assessed by an improvement in dyspepsia scores.

Statistical analysis
Only per-protocol population was included in statistical analysis.Treatment groups were compared with respect to the proportion of patients in whom eradication was successful using chi-square test (X 2 test).If a statistically significant difference was detected, pair-wise comparisons were carried out using 2 × 2 chi-square contingency table with Yate's (continuity) correction; the 95% confidence interval (95% CI) was also calculated.The different study groups were compared with respect to demographic data and endoscopic findings using ANOVA (one-way analysis of variance) for comparison of the means of age and BMI; and the chisquare (X 2 ) test, for comparison of the proportions of other categorical variables (sex, smoking, endoscopic findings).
The severity of dyspeptic symptoms before and after treatment was compared in each group using the Wilcoxon signed rank test.The post-treatment dyspeptic symptoms scores were compared between the four groups using Kruskal-Wallis test for ranked data.A P value of <0.05 was taken as significant.All statistical analyses were performed using the Statistical Package of Social Science (SPSS) version 11.

RESULTS
Of the 110 patients randomly assigned, 10 were excluded as they had to use antibiotics for some other ailment, and 11 discontinued because of either traveling abroad or loss of contact.One person was excluded during the course of investigation because of noncompliance.Thus 88 patients were finally recruited in the study.The treatment groups were well matched at baseline in relation to demographic and pretreatment clinical characteristics of the patients, including endoscopic findings; there were no statistically significant differences between the treatment groups [Table 1].
None of the patients withdrew from the study because of side effects or otherwise.Compliance was similar in all groups, and more than 98% of the patients took all the medications correctly.Only 1 patient was erratic in taking the medications, as assessed by frequent telephonic contact and counting the number of capsules remaining at the last visit.
Table 2 shows the eradication rates of different therapies in various groups of patients.The eradication of H. pylori was significantly better with TT compared to 1 g/d and 3 g/d of N. sativa, while it was statistically comparable to 2 g/d of N. sativa used for four weeks along with omeprazole.
As shown in Tables 3 and 4, the treatment regimens led to significant improvement of dyspeptic symptoms in 65%, 81%, 62% and 65% of patients in groups taking TT, 1 g/d, 2 g/d and 3 g/d of N. sativa, respectively (P < 0.001).None of the patients reported any worsening of the symptoms in any group.However, the comparison of post-treatment dyspeptic symptoms between the four groups did not reveal any significant difference.
Adverse effects recorded in the patients taking N. sativa

DISCUSSION
The rising prevalence of H. pylori antibiotic resistance emphasizes the need for discovery of new and safe modalities for treatment of H. pylori infection and the resulting gastroduodenal disease.Although H. pylori is susceptible to many antibiotics and plant products in vitro, not all can be used in vivo to eradicate the infection because of the highly peculiar characteristics and habitat of the organism and low pH of the stomach. [6]e present study was aimed at investigating the effect of N. sativa, supplied as ground seeds, in three doses (1 g/d, 2 g/d and 3 g/d, for 4 weeks) on H. pylori eradication in humans.
The results showed that a dose of 2 g/d eradicated H. pylori in infected patients in a proportion (67%) which is close to that of standard triple therapy.However, 1 g/d and 3 g/d N. sativa were significantly less effective than the triple therapy (P < 0.05) [Table 2] but still effective in approximately 48% of the patients.The triple therapy group in the present study achieved the highest rate for eradication of H. pylori (82.6%), which lies in the range reported by many recent studies investigating the efficacy of different triple therapy combinations. [5]The most effective and well-tolerated combination consists of amoxicillin, clarithromycin and omeprazole.Investigators have reported an eradication rate ranging from below 80% up to approximately 90%. [25,26]In general practice, 20% to 30% of therapies fail probably due to resistance or noncompliance of patients.These differences among various countries, despite using the same regimen, may be attributed to the presence or absence of pretreatment antimicrobial resistance. [27]olume 16, Number 3 Rajab 1431 H July 2010 The Saudi Journal of Gastroenterology to figure out the beneficial effects of N. sativa, if present, on the dyspeptic symptoms.

CONCLUSIONS
N. sativa ground seeds in a dose of 2 g/d when given along with 40 mg/d omeprazole possess clinically useful anti-Helicobacter pylori activity, comparable to that of the standard triple therapy.The doses of 1 g/d and 3 g/d of N. sativa were less effective, but the H. pylori eradication rate achieved with these doses was similar to that obtained with a single antibiotic.Further clinical trials employing N. sativa in combination with one of the antibiotics commonly used for eradication of H. pylori could prove to provide more efficient therapy that is relatively safer and less expensive.
The improvement of dyspeptic symptoms in our study was most probably due to the proton pump inhibitor, omeprazole, though N. sativa has previously been shown to possess gastroprotective and anti-secretory activities.

Table 4 :
Patients showing improvement in post-treatment score of refl ux symptoms with respect to pretreatment score in each study group Groups Number of patients showing change in refl ux symptoms score NS: N. sativa; O: Omeprazole; Triple therapy= (Amoxicillin + Clarithromycin + Omeprazole).n: number of patients.All groups showed signifi cant improvement in post-treatment score compared to the pretreatment score (P < 0.05) using Wilcoxon rank testand antibiotics were minor, similar, mostly related to gastrointestinal irritation and did not persist for long.

saudijgastro.com on Thursday, November 07, 2013, IP: 108.170.102.50] || Click here to download free Android application for this journalTable 1 : Baseline pretreatment characteristics of the patients Variable Group 1 TT (n=23) Group 2 1 g NS+O (n=21) Group 3 2g NS+O (n=21) Group 4 3 g NS+O (n=23) Differences between groups (P value)
NS:Nigella sativa; O: Omeprazole; GERD: gastroesophageal refl ux disease (according to LA classifi cation), n: number of patients; SD: standard deviation.Oneway analysis of variance (ANOVA) for comparison of the means of age and BMI.Chi-square (  2 ) test for comparison of the proportions of categorical variables (sex, smoking, endoscopic fi ndings).There were no signifi cant differences between the four groups regarding age, sex, BMI, smoking and endoscopic fi ndings

Table 3 : Patients showing improvement in post-treatment score of epigastric pain with respect to pretreatment score in each study group Groups Number of patients showing change in epigastric pain score P value Improved score Same score
NS: N. sativa; O: Omeprazole; Triple therapy= (Amoxicillin + Clarithromycin + Omeprazole).n: number of patients.* All groups showed signifi cant improvement in post-treatment score compared to the pretreatment score (P < 0.05) using Wilcoxon rank test