Cut and paste: A novel method of re-attaching rectus muscles with cyanoacrylate during recessions in strabismus

We compared time to reattachment (from disinsertion), complications and inflammatory scores (0 to +3: nil, mild, moderate and severe) on Day One, at two and at four to six weeks post surgery. Results: There were no significant group differences in inflammatory scores (Wilcoxon, all values of P >0.05). All attachments held firm. Gluing took significantly longer by 5.24±1.91 min (95% CI for difference: 3.87-6.61). There were no complications. Conclusion: We feel that although it takes marginally longer, iso-amyl cyanoacrylate offers an effective and safe alternative to sutures for muscle recession in strabismus surgery. Since it is cheaper (vs. polyglactin) and offers multi-use possibility it may also prove to be cost-effective.

A detailed history and examination was carried out, including visual acuity (VA), refraction, biomicroscopy and ophthalmoscopy. Strabismus-specific information such as amount of strabismus, status of binocularity (with Bagolini glasses) and any treatment undertaken in the form of spectacles, prism or patching was recorded.
Surgery was performed under standard peribulbar block. Using 5-0 silk traction sutures at 12 and 6 o'clock, the muscle was approached by the limbal approach. Prior to disinsertion, we passed a 6-0 double-armed Vicryl suture as follows: first a bite through the central belly was taken and then a whip stitch passed on the two edges of the tendon, near the insertion. We noted the time at disinsertion in order to calculate the length of time for reattachment of the muscle. For the study (IAC) group, we dried both the muscle and the sclera with cellulose strips and marked the point of required recession on the sclera after measuring with calipers. After applying one drop of IAC to both the scleral site and the cut edge of the muscle with a 25-gauge cannula [ Fig. 1], we held them in apposition for 45 sec [ Fig. 2]. In case there appeared to be a good 'take' evident from a good adhesion, no peeling-off appearance of the edge of the muscle, and a global movement on gently tugging at the (preplaced) 6-0 Vicryl suture, then time to completion of adhesion was noted (glue time: GT). In the event that the attachment failed, we planned to re-apply once more before switching to standard sutural recession surgery. The 6-0 Vicryl sutures were passed through the insertion stump as a backup option to be Cut and paste: A novel method of re-attaching rectus muscles with cyanoacrylate during recessions in strabismus used as an adjustable hang-back suture, in the unlikely event of the bioadhesive giving way. The ends were left long and carefully attached (with micropore) to the forehead at the end of surgery, and were to be removed after 4-6 h, if not needed.
In the eye constituting the control (PGS) group, we sutured the disinserted muscle to the sclera with the 6-0 Vicryl in a standard cross sword fashion. At the end, time to completion of suturing was noted (Vicryl time: VT). We reattached the conjunctiva at two points with Vicryl (8-0) suture. The eyes were patched for 4 to 6 h. A combination of ofloxacin (Oflacin, Microvision, India) with ketorolac (Ketoflox: Allergan, India) drops were applied four times a day for at least 15 days.
Outcomes assessed included conjunctival injection, chemosis, discomfort and discharge, on a scale of 0 to 3 (none, mild, moderate and severe) on Day one, at two weeks, and at four to six weeks postoperatively. In addition the sum of individual scores constituted a 'total score' (TS) with a range 0 to 12. Intra-operatively we recorded GT and VT, as also complications if any.
We applied the Wilcoxon test and paired t-test for analyses. Significance was set at P ≤ 0.05. Confidence intervals (95% CI) are quoted where appropriate.

Result
The demographic and clinical details of the 10 patients are outlined in Table 1. There were equal number of esotropes and exotropes. The median age was 18.5 years (range: 12-25).
Analyses of individual inflammatory scores revealed no significant difference between the IAC and PGS groups at any point of follow-up. This is borne out by the Wilcoxon paired analyses. P values for injection, chemosis, discomfort and discharge (in the order stated) were all >0.05: on Day one: 0.

Discussions
We could access only one comparable human study in the   literature done by Mulet. [19] In contrast to us, they used the cyanoacrylate, Adal-1 (a mix of ethylcarboxyacrylate + ethylcyanoacrylate). Unlike ours, theirs was an unpaired study on 27 eyes: 17 sutured versus 10 glued recession surgeries.
Animal studies have yielded evidence both against, [18] and in support of, [20,21] an effective bond between rectus muscles and sclera using cyanoacrylate. Although not part of the protocol, we are aware that even after six months, the glued eyes demonstrate equally good ductions and versions comparable to the sutured eyes, suggesting effective bonding.
We could not demonstrate any difference in individual inflammatory scores between sutured eyes and glued eyes. Only the TS on the first day marginally favored the IAC group, and although statistically significant, the result was too small to be clinically meaningful. Comparative bio-tolerance has also been demonstrated in rabbits and humans, with statistically no significant difference in postoperative inflammatory reaction between cyanoacrylate and sutures. [4,19] Though we did not measure the muscular displacement from the points of attachment, both Mulet (in humans) and Tolleni (in rabbits) failed to demonstrate any significant displacement after gluing. [20,21] Compared to suturing, on an average, it took statistically significantly longer to glue back the muscles. We feel that a difference of a mere five minutes on an average would not be of much clinical importance. We are of the opinion that diligent cauterization of bleeders, (cautery was not part of our protocol) is likely to markedly decrease the total time for attachment with glue. We could not find any study where duration of surgery had been studied.
Although we did not undertake formal cost-effectiveness analysis, we estimate that use of IAC is likely to be cheaper than the use of Vicryl sutures: One 0.25 ml vial of IAC costs Rs. 250, and can suffice for both the eyes compared to Rs. 940 for the cost of two Vicryl sutures for two eyes. Clustering the patients on a particular day may allow greater cost reduction, since one IAC vial can be used in up to four patients.
We are only too aware of our small sample, and recommend that more studies with larger number of patients, incorporating parametric statistical testing, are needed to further corroborate our findings.
We are confident that in the future cyanoacrylate can be tried for rectus resections in addition to recessions. Its use should also be considered for re-attaching the conjunctiva to make the strabismus surgery completely sutureless.
Our study, therefore, provides evidence that supports the successful use of cyanoacrylate as a suitable bio-tolerant alternative to sutures to re-attach rectus muscles during recessions in strabismus surgery.