Download PDF
CC BY-NC-ND 4.0 · Indian J Plast Surg 2014; 47(02): 216-220
DOI: 10.4103/0970-0358.138952
Original Article
Association of Plastic Surgeons of India

Effect of limited access dressing on hydroxyproline and enzymatic antioxidant status in nonhealing chronic ulcers

Thittamaranahalli Muguregowda Honnegowda
Departments of Plastic Surgery and Burns, Kasturba Medical College, Manipal, Karnataka, India
,
Pramod Kumar
Departments of Plastic Surgery and Burns, Kasturba Medical College, Manipal, Karnataka, India
,
Padmanabha Udupa
1   Departments of Biochemistry, Kasturba Medical College, Manipal, Karnataka, India
,
Pragna Rao
1   Departments of Biochemistry, Kasturba Medical College, Manipal, Karnataka, India
,
Siddhartha Bhandary
2   Departments of Surgery, Kasturba Medical College, Manipal, Karnataka, India
,
Krishna Kumar Mahato
3   Departments of Biophysics, Kasturba Medical College, Manipal, Karnataka, India
,
Anurag Sharan
Departments of Plastic Surgery and Burns, Kasturba Medical College, Manipal, Karnataka, India
,
Shreemathi S. Mayya
4   Departments of Biostatistics, Kasturba Medical College, Manipal, Karnataka, India
› Author Affiliations
Further Information

Publication History

Publication Date:
26 August 2019 (online)

Also available at
   Permissions and Reprints

ABSTRACT

Background: Healing ability of nonhealing chronic ulcers can be assessed by estimating hydroxyproline, total protein and enzymatic antioxidants such as glutathione peroxidase (GPx), glutathione S-transferase (GST) in the granulation tissue. Materials and Method: A total of 34 patients were analysed from two groups: Limited access dressing (LAD) group (n = 17) and conventional dressing group (n = 17). Results: Patients treated with LAD that exerts combination of intermittent negative pressure and moist wound-healing had shown a significant increase in the hydroxyproline (P = 0.026), total protein (P = 0.004), GPx level (P = 0.030) and GST level (P = 0.045). Conclusion: Patients treated with LAD indicated significantly better anabolic effect on wound-healing compared to that of patients treated with conventional dressing.

KEY WORDS

Chronic ulcers - chronic wound-healing - enzymatic antioxidants - hydroxyproline - limited access - dressing - total protein
 

  • REFERENCES

  • 1 Goldberg SR, Diegelmann RF. Wound healing primer. Surg Clin North Am 2010; 90: 1133-46
    CrossrefPubMedGoogle Scholar
  • 2 Armstrong DG, Lavery LA. Diabetic Foot Study Consortium. Negative pressure wound therapy after partial diabetic foot amputation: A multicentre, randomised controlled trial. Lancet 2005; 366: 1704-10
    CrossrefPubMedGoogle Scholar
  • 3 Mouës CM, van den Bemd GJ, Meerding WJ, Hovius SE. An economic evaluation of the use of TNP on full-thickness wounds. J Wound Care 2005; 14: 224-7
    CrossrefPubMedGoogle Scholar
  • 4 Leininger BE, Rasmussen TE, Smith DL, Jenkins DH, Coppola C. Experience with wound VAC and delayed primary closure of contaminated soft tissue injuries in Iraq. J Trauma 2006; 61: 1207-11
    CrossrefPubMedGoogle Scholar
  • 5 Apelqvist J, Armstrong DG, Lavery LA, Boulton AJ. Resource utilization and economic costs of care based on a randomized trial of vacuum-assisted closure therapy in the treatment of diabetic foot wounds. Am J Surg 2008; 195: 782-8
    CrossrefPubMedGoogle Scholar
  • 6 Braakenburg A, Obdeijn MC, Feitz R, van Rooij IA, van Griethuysen AJ, Klinkenbijl JH. The clinical efficacy and cost effectiveness of the vacuum-assisted closure technique in the management of acute and chronic wounds: A randomized controlled trial. Plast Reconstr Surg 2006; 118: 390-7
    CrossrefPubMedGoogle Scholar
  • 7 Park CA, Defranzo AJ, Marks MW, Molnar JA. Outpatient reconstruction using integraFNx01 and subatmospheric pressure. Ann Plast Surg 2009; 62: 164-9
    CrossrefPubMedGoogle Scholar
  • 8 Hurd T, Chadwick P, Cote J, Cockwill J, Mole TR, Smith JM. Impact of gauze-based NPWT on the patient and nursing experience in the treatment of challenging wounds. Int Wound J 2010; 7: 448-55
    CrossrefPubMedGoogle Scholar
  • 9 Kumar P. Limited access dressing and maggot. Wounds 2009; 21: 150-2
    PubMedGoogle Scholar
  • 10 Kumar P, Sharma A. The limited access dressing for damage control in trauma patients. Wounds 2010; 22: 188-92
    PubMedGoogle Scholar
  • 11 Joseph E, Hamori CA, Bergman S, Roaf E, Swann NF, Anastasi GW. A prospective, randomized trial of vacuum assisted closure versus standard therapy of chronic non healing wounds. Wounds 2000; 12: 6-7
    Google Scholar
  • 12 Ford CN, Reinhard ER, Yeh D, Syrek D, De Las Morenas A, Bergman SB. et al. Interim analysis of a prospective, randomized trial of vacuum-assisted closure versus the healthpoint system in the management of pressure ulcers. Ann Plast Surg 2002; 49: 55-61
    CrossrefPubMedGoogle Scholar
  • 13 Armstrong DG, Lavery LA, Boulton AJ. Negative pressure wound therapy via vacuum-assisted closure following partial foot amputation: What is the role of wound chronicity?. Int Wound J 2007; 4: 79-86
    CrossrefPubMedGoogle Scholar
  • 14 Neuman RE, Logan MA. The determination of collagen and elastin in tissues. J Biol Chem 1950; 186: 549-56
    CrossrefPubMedGoogle Scholar
  • 15 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-75
    CrossrefPubMedGoogle Scholar
  • 16 Abstract Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Science 1973; 179: 588-90
    CrossrefPubMedGoogle Scholar
  • 17 Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 25 (249) 7130-9
    Google Scholar
  • 18 Kumar P, Honnegowda TM, Udupa EG, Rao P, Saran A, Bhandari S. The role of matrix metalloproteinase in the chronic wound healing. J Soc Wound Care Search 2012; 5: 7-16
    Google Scholar
  • 19 Wenk J, Foitzik A, Achterberg V, Sabiwalsky A, Dissemond J, Meewes C. et al. Selective pick-up of increased iron by deferoxamine-coupled cellulose abrogates the iron-driven induction of matrix-degrading metalloproteinase 1 and lipid peroxidation in human dermal fibroblasts in vitro: A new dressing concept. J Invest Dermatol 2001; 116: 833-9
    CrossrefPubMedGoogle Scholar
  • 20 Robson MC, Mustoe TA, Hunt TK. The future of recombinant growth factors in wound healing. Am J Surg 1998; 176: 80S-2
    PubMedGoogle Scholar
  • 21 Falanga V. Growth factors and wound healing. Dermatol Clin 1993; 11: 667-75
    CrossrefPubMedGoogle Scholar
  • 22 Kumar R, Katoch SS, Sharma S. Beta-adrenoceptor agonist treatment reverses denervation atrophy with augmentation of collagen proliferation in denervated mice gastrocnemius muscle. Indian J Exp Biol 2006; 44: 371-6
    PubMedGoogle Scholar
  • 23 Prasad SK, Kumar R, Patel DK, Hemalatha S. Wound healing activity of Withania coagulans in streptozotocin-induced diabetic rats. Pharm Biol 2010; 48: 1397-404
    CrossrefPubMedGoogle Scholar
  • 24 Gupta A, Kumar R, Pal K, Banerjee PK, Sawhney RC. A preclinical study of the effects of seabuckthorn (Hippophae rhamnoides L.) leaf extract on cutaneous wound healing in albino rats. Int J Low Extrem Wounds 2005; 4: 88-92
    CrossrefPubMedGoogle Scholar
  • 25 Gurung S, Skalko-Basnet N. Wound healing properties of Carica papaya latex: in vivo evaluation in mice burn model. J Ethnopharmacol 2009; 121: 338-41
    CrossrefPubMedGoogle Scholar
  • 26 Reddy GK, Stehno-Bittel L, Enwemeka CS. Laser photostimulation accelerates wound healing in diabetic rats. Wound Repair Regen 2001; 9: 248-55
    CrossrefPubMedGoogle Scholar
  • 27 Alvarez OM, Mertz PM, Eaglstein WH. The effect of occlusive dressings on collagen synthesis and re-epithelialization in superficial wounds. J Surg Res 1983; 35: 142-8
    CrossrefPubMedGoogle Scholar
  • 28 Argenta LC, Morykwas MJ. Vacuum-assisted closure: A new method for wound control and treatment: Clinical experience. Ann Plast Surg 1997; 38: 563-76
    CrossrefPubMedGoogle Scholar
  • 29 Adamson B, Schwarz D, Klugston P, Gilmont R, Perry L, Fisher J. et al. Delayed repair: The role of glutathione in a rat incisional wound model. J Surg Res 1996; 62: 159-64
    CrossrefPubMedGoogle Scholar
  • 30 Halliwell B, Grootveld M, Gutteridge JM. Methods for the measurement of hydroxyl radicals in biomedical systems: Deoxyribose degradation and aromatic hydroxylation. Methods Biochem Anal 1988; 33: 59-90
    PubMedGoogle Scholar