Osteoblastic Cells Response to Albumin Coatings on Zinc Containing Hydroxyapatite

Andrea Machado Costa; Elena Mavropoulos; Marcelo Tanaka; Daniel Navarro da Rocha; Flavia Mendonça Fonseca; Marcelo Henrique Prado da Silva

doi:10.4028/www.scientific.net/KEM.720.120

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In Vitro Characterization of 3D Beta-Tricalcium Phosphate Scaffolds Reinforced with Phosphate Based-Bioactive Glass for Bone Replacement
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Synthesis and In Vitro Characterization of Carbon Nano Tube-Polycaprolactone Composite Scaffold for Odontoblast Cell Interaction
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Osteoblastic Cells Response to Albumin Coatings on Zinc Containing Hydroxyapatite
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Simultaneous Identification of Amorphous Calcium Phosphate and S.epidermidis Bacteria by Photoacoustic Spectroscopy
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Characteristics and Biocompatibility of the Hydroxyapatite Based Two Ternary Biocomposites
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Basic Properties of Novel Bioactive Cement Based on Silica-Calcium Phosphate Composite and Carbonate Apatite
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 720Osteoblastic Cells Response to Albumin Coatings on...

Osteoblastic Cells Response to Albumin Coatings on Zinc Containing Hydroxyapatite

Abstract:

In this work, zinc-containing hydroxyapatite (ZnHA) and hydroxyapatite (HA) discs were coated with native bovine serum albumin (BSA) using 100 μg/ml BSA solution at 37°C. Changes on BSA secondary structure from α-helix to β-turn were detected by deconvolution analyses of FTIR amide I band. Fluorescence images of MC3T3-E1 osteoblast cultured on HA and ZnHA discs revealed cells with elongated morphology, typical of 2D experiments, after 24 hours. An intense cytoskeleton organization was observed on samples coated with BSA.

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Periodical:

Key Engineering Materials (Volume 720)

Pages:

120-124

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.720.120

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Online since:

November 2016

Authors:

Andrea Machado Costa*, Elena Mavropoulos, Marcelo Tanaka, Daniel Navarro da Rocha, Flavia Mendonça Fonseca, Marcelo Henrique Prado da Silva

Keywords:

Albumin, Hydroxyapatite, MC3T3-E1 Cells, Zinc

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* - Corresponding Author

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