Distribution & Access For Publication Downloads News About Us Contact Us
Paper Titles
Preface
Physico-Chemical Properties and Characterization of CaO-Fe2O3-P2O5 Glass as a Bioactive Ceramic Material
p.1
Biodegradable Magnesium Alloys: A Review of Material Development and Applications
p.25
Effect of Biomimetic Nanocrystalline Apatite Surface on Bone Ingrowth
p.41
Synthesis and Characterization of Sol-Gel Derived Hydroxyapatite-Bioglass Composite Nanopowders for Biomedical Applications
p.51
Tooth Tissue Engineering: Potential and Pitfalls
p.59
Finite Element Modelling of Rheological Property of Curing PMMA Bone Cement. Part 1 - Effect of Prosthesis Insertion Velocity
p.83
An Investigation of the Carriage Rate of Methicillin-Resistant Staphylococcus aureus in Pigs in the Western Province of Vietnam
p.91

An Investigation of the Carriage Rate of Methicillin-Resistant Staphylococcus aureus in Pigs in the Western Province of Vietnam

Article Preview

Abstract:

Methicillin-resistant Staphylococcus aureus (MRSA) transmission between pigs and humans can be a dangerous infection source for the community. To control and prevent such disease, MRSA research in healthy pigs has been conducted in the US and Holland. This paper reports the first such study in Vietnam. This study aims to optimize the method for investigating MRSA carriage in tonsil and nasal swabs of healthy pigs. Potential samples were selected rapidly by Multiplex PCR (M-PCR) assay with 3 primers (Staph 756, mecA, Sa442) and then combined with conventional methods to detect and pick up MRSA strains. These strains were grouped based on genome pattern by pulsed field gel electrophoresis (PFGE). This study showed that we successfully isolated MRSA and the rate of MRSA carriage samples was 3.63%, much lower than that reported in the USA (49%) and Holland (39%), however the USA and Holland studies investigated pigs from industrial scale piggeries, whereas our study investigated pigs from small farms, which is the usual approach to pig farming in Vietnam. The MRSA patterns identified and associated MRSA strains were different from identified MRSA strains in other countries. This research provides important information about the carriage rate of MRSA in pigs in the Western province of Vietnam and demonstrates the importance of ongoing investigations in this area.

You might also be interested in these eBooks
Journal of Biomimetics, Biomaterials & Tissue Engineering Vol.12 View Preview

Info:

Periodical:

Journal of Biomimetics, Biomaterials and Tissue Engineering (Volume 12)

Pages:

91-98

DOI:

https://doi.org/10.4028/www.scientific.net/JBBTE.12.91

Citation:

Cite this paper

Online since:

February 2012

Authors:

Thi Diem Thi Vo, An Doan Mai Huynh, Hoa Thi Ngo, Duong Thuy Nguyen, Thi Minh Tam Tran

Keywords:

Methicillin-Resistant Staphylococcus aureus (MRSA), Methicillin-Sensitive Staphylococcus aureus, Multiplex PCR, Pulsed Field Gel Electrophoresis

Export:

RIS, BibTeX

Price:

Permissions:

Request Permissions

References

[1] S. Cui, J. Li, C. Hu, S. Jin, F. Li. Y. Guo, L. Ran, Y. Ma; Isolation and characterization of methicillin-resistant Staphylococcus aureus from swine and workers in China. J. Antimicrob. Chemother; 2009, 64 (4), 680-3.

DOI: 10.1093/jac/dkp275

Google Scholar

[2] T. Ito, Y. Katayama, N. More, K. Tsutsumimoto, C. Tiensasitorn, K. Hiramatsu; Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother; 2001, 45 (5), 1323-36.

DOI: 10.1128/aac.45.5.1323-1336.2001

Google Scholar

[3] T. Khanna, R. Friendship, C. Dewey, J. S Weese; Methicillin resistant Staphylococcus aureus colonization in pigs and pig farmers. Vet Microbiol; 2008, 128 (3-4), 298-303.

DOI: 10.1016/j.vetmic.2007.10.006

Google Scholar

[4] C. M Vandenbroucke-Grauls, D. J Beaujean; Methicillin-resistant Staphylococcus aureus in pig breeders and cattle breeders. Ned. Tijdschr. Geneeskd; 2006, 150 (31), 1710-2.

Google Scholar

[5] A. Voss, F. Loeffen, J. Bakker, C. Klaassen, M. Wulf; Methicillin-resistant Staphylococcus aureus in Pig Farming. Emerg Infect Dis; 2005, 11 (12), 1965-6.

DOI: 10.3201/eid1112.050428

Google Scholar

[6] M. Wulf, A. van Ness, A. Eikelenboom-Boskamp, J. de Vries, W. Melchers, C. Klaassen, A. Voss; Methicillin resistant Staphylococcus aureus in Veterinary Doctors and Students, the Netherlands. Emerg Infect Dis, 2006, 12 (12), 1939-41.

DOI: 10.3201/eid1212.060355

Google Scholar

[7] C. C Bens, A. Voss, C. H Klaassen; Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed field gel electroforesis analysis. J Clin Microbiol; 2006, 44 (5), 1875-6.

DOI: 10.1128/jcm.44.5.1875-1876.2006

Google Scholar

[8] K. Krziwanek, S. Metz-Gercek, H. Mittermayer; Methicillin-Resistant Staphylococcus aureus ST398 from Human Patients, Upper Austria. Emerg Infect Dis; 2005, 15 (5), 766-9.

DOI: 10.3201/eid1505.080326

Google Scholar

[9] K. Zhang, J. Sparling, B. L Chow, S. Elsayed, Z. Hussain, D. L Church, D. B Gregson, T. Louie, J. M Conly; New Quadriplex PCR assay for Methicillin and Mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from Coagulase-negative Staphylococci. J Clin Microbiol; 2004, 42 (11), 4947-55.

DOI: 10.1128/jcm.42.11.4947-4955.2004

Google Scholar

[10] U. Reischl, H. J Linde, M. Metz, N. Lehn; Rapid identification of Methicillin resistant Staphylococcus aureus and simultaneous species confirmation using Real-time Fluorescence PCR. J Clin Microbiol; 2000, 38 (6), 2429-33.

DOI: 10.1128/jcm.38.6.2429-2433.2000

Google Scholar

[11] T. C Thuong, D. T Nguyen, T. H Ngo, T. M Nguyen, V. T Le, S. D To, J. Lindsay, T. D Nguyen, V. C Bach, Q. T Le, T. H Le, D. L Le, J. Campbell, T. K Nguyen, V. V Nguyen, J. Cockfield, T. G Le, V. N Phan, H. S Le, V. P Le, M. Counahan, A. Bentsi-Enchill, R. Brown, J. Simmerman, T. C Nguyen, T. H Tran, J. Farrar, C. Schultsz; An outbreak of severe Infections with Community-Acquired MRSA Carrying the Panton-Valentine Leukocidin Following Vaccination. Plos One; 2007, 9, e822.

DOI: 10.1371/journal.pone.0000822

Google Scholar

[12] F. C Tenover, R. D Arbeit, R. V Goering, P. A Mickelsen, B. E Murray, D. H Persing, B. Swaminathan; Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacteria strain typing. J Clin Microbiol, 1995, 33 (9), 2233-9.

DOI: 10.1128/jcm.33.9.2233-2239.1995

Google Scholar

[13] J. A McClure, J. M Conly, V. Lau, S. Elsayed, T. Louie, W. Hutchins, K. Zhang; Novel Multiplex PCR Assay for Detection of the Staphylococcal Virulence Marker Panton-Valentine Leukocidin Genes and Simultaneous Discrimination of Methicillin-Susceptible from -Resistant Staphylococci. J. Clinical Microbiol; 2006, 44 (3), 1141-4.

DOI: 10.1128/jcm.44.3.1141-1144.2006

Google Scholar

[14] A. J de Neeling, M. J van den Broel., E. C Spalburg, M. G van Santen-Verheuvel, W. D Dam-Deisz, H. C Boshuizen, A. W van de Giessen, E. van Duijkeren, X. W Huijsdens; High prevalence of methicillin resistant Staphylococcus aureus in pigs. Vet Microbiol; 2007, 122 (3-4), 366-72.

DOI: 10.1016/j.vetmic.2007.01.027

Google Scholar

[15] T.C. Smith, M. J Male, A. L Harper, J. S Kroeger, G. P Tinkler, E. D Moritz, A. W Capuano, L. A Herwaldt, D. J Diekema; Methicillin-Resistant Staphylococcus aureus (MRSA) Strain ST398 is Present in Midwestern U.S. Swine and Swine Workers. Plos One, 2009, 4 (1), e4258.

DOI: 10.1371/journal.pone.0004258

Google Scholar

[16] X. W Huijsdens, B. J van Dijke, E. Spalburg, M. G van Santen-Verheuvel, M. E Heck, G. N Pluister, A. Voss, W. J Wannet, A. J de Neeling; Community-acquired MRSA and pig-farming. Ann Clin Microbiol Antimicrob; 2006, 5: 26.

DOI: 10.1186/1476-0711-5-26

Google Scholar

[17] L. Guardabassi, M. Stegger, R. Skov; Retrospective detection of methicillin resistant and susceptible Staphylococcus aureus ST398 in Danish slaughter pigs. Vet Microbiol; 2007, 122 (3-4), 384–6.

DOI: 10.1016/j.vetmic.2007.03.021

Google Scholar

Scientific.Net is a registered brand of Trans Tech Publications Ltd
© 2024 by Trans Tech Publications Ltd. All Rights Reserved