Screening of Antarctic Antagonism against Vibro anguillarum and Preliminary Research of the Antibacterial Substances

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Abstract:

Vibrio anguillarum is an opportunistic pathogen of aquatic animals, biocontrol agents have been revealed effective in preventing this disease. Antarctic bacteria were used for the screening of antagonism against V. anguillarum and strain AN32 showed highest activity. AN32 was identified as Pseudoalteromonas according to its morphological, physiological and biochemical and 16S rDNA molecular properties. The antibacterial substance in AN32 culture supernatant was sensitive to pH, temperature and proteinases. The substance could not dissolve in organic solvents, but could be precipitated by ammonium sulfate. These characterizations indicated that the bioactive substance of strain AN32 would be some kind of protein or peptide. This study is a potential application in the control of aquiculture diseases.

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Periodical:

Advanced Materials Research (Volumes 347-353)

Pages:

635-638

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Online since:

October 2011

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[1] J. Bolinches, A.E. Toranzo, A. Silva and J.L. Barja: Bull. Eur. Assoc. Fish Pathol. Vol. 6 (1986), p.1–4.

Google Scholar

[2] S.M. Denkin and D.R. Nelson: Applied and Environmental Microbiology Vol. 70 (2004) No.7, p.4193–4204

Google Scholar

[3] F.L. Thompson, T. Iida and J. Swings: Microbiology and Molecular Biology Reviews Vol. 68 (2004) No.3, p.403–431.

Google Scholar

[4] B. Austin and D.A. Austin: Bacterial Fish Pathogens: Diseases of Farmed and Wild Fish, 4th ed. (Praxis Publishing Ltd, Chichester, UK 2007)

Google Scholar

[5] L. Gram, J. Melchiorsen, B. Spanggaard, I. Huber and T.F. Nielsen: Applied and Environmental Microbiology Vol. 65 (1999) No.3, p.969–973.

DOI: 10.1128/aem.65.3.969-973.1999

Google Scholar

[6] M. Hjelm, Ø. Bergh, A. Riaza, J. Nielsen, J. Melchiorsen, S. Jensen, H. Duncan, P. Ahrens, H. Birkbeck and L. Gram: System. Appl. Microbiol. Vol. 27 (2004), p.360–371.

DOI: 10.1078/0723-2020-00256

Google Scholar

[7] M. Planas, M. Pérez-Lorenzo, M. Hjelm, L. Gram, I.U. Fiksdal, Ø. Bergh and J. Pintado: Aquaculture Vol. 255 (2006), pp.323-333.

DOI: 10.1016/j.aquaculture.2005.11.039

Google Scholar

[8] M.C. Torrento and J.L. Torres: UPV Journal of Natural Sciences Vol. 1 (1996), p.130–138.

Google Scholar

[9] R.Chythanya, I. Karunasagar and I. Karunasagar: Aquaculture Vol. 208 (2002), p.1–10.

Google Scholar

[10] R. Preetha, S. Jose, S. Prathapan, K.K. Vijayan, N. Jayaprakash, R. Philip and I.S. Bright Singh: Aquaculture Research Vol. 41 (2010), p.1452–1461.

DOI: 10.1111/j.1365-2109.2009.02436.x

Google Scholar