Preparation and Characterization of Hybrid Fe3O4/APTES for Immobilization of GOX

Article Preview

Abstract:

This work aims to obtain hybrid from nanoparticles of Fe3O4 modified surface with 3-aminopropyltriethoxysilane (APTES) for their use in the immobilization of glucose oxidase (GOX). The obtained material was characterized by XRD, FTIR, SEM, sedimentation test and magnetic separation. Based on the obtained results it showed that the surface modification with APTES was successfully achieved and that the APTES did not changed the magnetic characteristic neither the structure of Fe3O4, indicating that the hybrid material obtained is promising for immobilization of GOX.

You might also be interested in these eBooks

Info:

Periodical:

Materials Science Forum (Volumes 798-799)

Pages:

460-465

Citation:

Online since:

June 2014

Export:

Price:

* - Corresponding Author

[1] American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 31 (2008) S55-S60.

DOI: 10.2337/dc08-s055

Google Scholar

[2] E. S. Huang, A. Basu, M. O`Grady, J. C. Capretta, Projecting the future diabetes population size and related costs for the U.S. Diabetes Care 32 (2009) 2225-2229.

DOI: 10.2337/dc09-0459

Google Scholar

[3] L. Vilar, Endocrinologia Clinica, 3ª. ed. Guanabara Koogan, Rio de Janeiro, (2006).

Google Scholar

[4] M. B. Gomes, A. C. Lerario, Métodos para monitorar o tratamento da hiperglicemia. Diretrizes da Sociedade Brasileira de Diabetes, 3ª. ed., (2009).

Google Scholar

[5] P. D. Orazio, Biosensor in clinical chemistry. Clinica Chimica Acta 334 (2003) 41-69.

Google Scholar

[6] F. S. Sheu, S. K. Vashist, D. Zheng, K. Al-Rubeaan, J. H. T. Luong, Technology behind commercial devices for blood glucose monitoring in diabetes management: A review. Analytica Chimica Acta 703 (2011) 124-136.

DOI: 10.1016/j.aca.2011.07.024

Google Scholar

[7] S. K. Vashist, Non-invasive glucose monitoring technology in diabetes management: A review. Analytica Chimica Acta 750 (2012) 16-27.

DOI: 10.1016/j.aca.2012.03.043

Google Scholar

[8] N. C. S. Vieira, Biossensores de Glicose Nanoestruturados Baseados em Dendrímeros PAMAM e Filmes finos de In2O3: Sn. 141f. Dissertação apresentada ao Curso de Mestrado da Universidade Federal de Itajubá. (2006).

DOI: 10.11606/t.76.2012.tde-10022012-151657

Google Scholar

[9] N. M. José, L. A. S. A. Prado, Materiais híbridos orgânico-inorgânicos: preparação e algumas aplicações. Química Nova 28 (2005) 281-288.

DOI: 10.1590/s0100-40422005000200020

Google Scholar

[10] S. Kanimozhi, K. Perinbam, Synthesis of amino-silane modified superparamagnetic Fe3O4 nanoparticles and its application in immobilization of lipase from Pseudomonas fluorescens Lp1. Materials Research Bulletin (2013).

DOI: 10.1016/j.materresbull.2013.01.024

Google Scholar

[11] J. M. Pingarrón, P. Díez, R. Villalonga, M. L. Villalonga, Supramolecular immobilization of redox enzymes on cyclodextrin-coated magnetic nanoparticles for biosensing applications. Journal of Colloid and Interface Science 386 (2012) 181-188.

DOI: 10.1016/j.jcis.2012.07.050

Google Scholar

[12] K. S. Kumar, M. Seenuvasan, C. G. Malar, S. Preethi, N. Balaji, J. Iyyappan, M. A. Kumar, Fabrication, characterization and application of pectin degrading Fe3O4–SiO2 nanobiocatalyst. Materials Science and Engineering C 33 (2013) 2273-2279.

DOI: 10.1016/j.msec.2013.01.050

Google Scholar