Preparation of Porous Hydroxyapatite as Synthetic Scaffold Using Powder Deposition and Sintering and Cytotoxicity Evaluation

Eko Pujiyanto; Alva Edy Tontowi; Muhammad Waziz Wildan; Widowati Siswomihardjo

doi:10.4028/www.scientific.net/AMR.747.123

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 747Preparation of Porous Hydroxyapatite as Synthetic...

Preparation of Porous Hydroxyapatite as Synthetic Scaffold Using Powder Deposition and Sintering and Cytotoxicity Evaluation

Abstract:

This study prepared porous hydroxyapatite (porous HA) as synthetic scaffold and find out chemical properties, porosity, compressive strength and cytotoxicity properties. Porous HA was prepared by powder deposition and sintering from HA-PMMA mixed powder. Porous HA characterizations were conducted by XRD, XRF, SEM-EDX and mercury porosimetry analysis. In vitro cytotoxicity testing of porous HA was conducted by MTT method using vero cells. Porous HA has porosity on the interval 62.79 to 69.67% and compressive strength on the interval 1.53 to 3.71 MPa. Optimal porous HA has porosity is 62.79% with compressive strength is 3.71 MPa. Mercury porosimetry analysis showed that optimal porous HA has interconnective porosity up to 88.25% with pore size on the interval 0.05-355 μm and median pore is 52.64 μm. There was no significantly difference in the death percentage of vero cells caused HA powder and optimal porous HA (p= 0.158) but concentration of optimal porous HA were significantly effect on the percentage of vero cells death (p=0.003).

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Periodical:

Advanced Materials Research (Volume 747)

Pages:

123-126

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.747.123

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Online since:

August 2013

Authors:

Eko Pujiyanto, Alva Edy Tontowi, Muhammad Waziz Wildan, Widowati Siswomihardjo

Keywords:

Porous Hydroxyapatite, Powder Cytotoxicity, Powder Deposition, Synthetic Scaffold

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References

[1] S. N. Parikh, Bone Graft Substitutes: Past, Present and Future, Journal Postgraduate Medicine. 48 (2002) 142-148.

Google Scholar

[2] I. Sopyan, M. Mel, S. Ramesh, K. A. Khalid, Porous Hydroxyapatite for Artificial Bone Application, Science and Technology of Advanced Materials. 8 (2007) 116-123.

DOI: 10.1016/j.stam.2006.11.017

Google Scholar

[3] S. F. Hulbert, S. J. Morisson, J. J. Klawitter, Tissue Reaction to Three Ceramics of Porous and Non-Porous Structures, Journal Biomedical Material Research. 6 (1972) 347-354.

DOI: 10.1002/jbm.820060505

Google Scholar

[4] M. Doblare, J. M Garcıa., M.J. Gomez, Modelling Bone Tissue Fracture and Healing: A Review, Engineering Fracture Mechanics. 71 (2004) 1809–1840.

DOI: 10.1016/j.engfracmech.2003.08.003

Google Scholar

[5] B. S. Chang, C. K. Lee, K. S. Hong, H. J. Youn, H. S. Ryu, S. S. Chung, K. W. Park, Osteoconduction at Porous Hydroxyapatite with Various Pore Configurations, Biomaterials. 21 (2000) 1291-1298.

DOI: 10.1016/s0142-9612(00)00030-2

Google Scholar

[6] H. Katsuki,S. Furuta, Microwave Versus Conventional Hydrothermal Synthesis of Hydroxyapatite Crystals from Gypsum, Journal American Ceramic Society. 87 (1999) 2257-2259.

DOI: 10.1111/j.1151-2916.1999.tb02073.x

Google Scholar

[7] E. Pujiyanto, W. Siswomihardjo , I. D. Ana , A. E. Tontowi and M.W. Wildan, Cytotoxicity of Hydroxyapatite Synthesized from Local Gypsum, Proceedings BME Days. (2006) 92-95.

Google Scholar

[8] E. Pujiyanto, W. Siswomihardjo , I. D. Ana , A. E. Tontowi and M.W. Wildan, Porous Hydroxyapatite–Zirconia Composites Prepared by Powder Deposition and Pressureless Sintering, Advanced Materials Research 445 (2012) 463-468.

DOI: 10.4028/scientific5/amr.445.463

Google Scholar

[9] S. Junedi, Document Number CCRC-02-010-00, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, (2011).

Google Scholar

[10] D. E. C. Corbridge, Phosphorus: an Outline of Its Chemistry, Biochemistry and Technology, Elsevier, Amsterdam, (1990).

Google Scholar