Immobilization of Peroxidase from Cauliflower Stem on Ultrafiltration Membrane for Phenol Removal

Karnika Ratanapongleka; Apinya Onsarn

doi:10.4028/www.scientific.net/AMM.879.137

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 879Immobilization of Peroxidase from Cauliflower Stem...

Immobilization of Peroxidase from Cauliflower Stem on Ultrafiltration Membrane for Phenol Removal

Abstract:

The aim of this work is to investigate phenol removal by immobilized peroxidase extracted from cauliflower stem. Peroxidase was partially purified by membrane filtration and diafiltration. Almost four-fold increase in the measured activity of partially purified peroxidase was obtained. The enzyme was then immobilized on to the surface of regenerated cellulose ultrafiltration membrane (molecular weight cut-off 30 kDa) using a dead-end filtration unit. Three different immobilization methods (physical adsorption, cross-linking and covalent-bonding using glutaraldehyde as a membrane activator) were tested. The immobilization and enzymatic reaction efficiency were evaluated in terms of the immobilization yield, the enzyme leakage from the system, the phenol removal and the permeate flux. Results showed that the immobilization methods did not much affect the permeate flux of the membrane. The peroxidase immobilization by covalent-bonding on regenerated cellulose membrane produced the highest immobilization yield and the lowest enzyme leakage. The immobilized enzymatic reaction efficiency on phenol removal was 100% at operational time 60 min and reduced to 96.4% at 600 min.

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Periodical:

Applied Mechanics and Materials (Volume 879)

Pages:

137-143

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.879.137

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Online since:

March 2018

Authors:

Karnika Ratanapongleka*, Apinya Onsarn

Keywords:

Cauliflower Stem, Immobilization, Peroxidase, Phenol, Ultrafiltration Membrane

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