Gestational Ingestion of Oxidized Frying Oil by C57BL/6J Mice Differentially Affects the Susceptibility of the Male and Female Offspring to Diet-Induced Obesity in Adulthood2

https://doi.org/10.3945/jn.112.168948Get rights and content
Under an Elsevier user license
open archive

Abstract

The aim of this study was to investigate whether maternal ingestion of oxidized frying oil (OFO) during pregnancy influences the susceptibility to diet-induced obesity (DIO) of the adult offspring. Pregnant C57BL/6J mice were fed either a control diet [10% fresh soybean oil (SO)] or an OFO-containing diet (10% OFO) throughout the entire gestational period. After parturition, all pups were nursed by SO-fed dams for 3 wk, weaned onto a nonpurified standard diet for 4 wk, and shifted to a high-fat diet (29% butter + 1% SO) for 5 wk. Consequently, 4 groups of offspring were obtained, consisting of the male (m) or female (f) offspring of dams fed the OFO diet (OFO-m and OFO-f) or the SO diet (SO-m and SO-f). At pregnancy d 18, higher amounts (P < 0.05) of mRNA for PPARα target genes were found in the liver of the OFO-fed dams and their fetuses than in their SO controls. Although all pups were raised under the same conditions in postnatal life, a comparison based on the gender of pups from dams fed the different diets showed that adult OFO-f mice were prone to DIO, whereas adult OFO-m mice were resistant. The adult OFO-m mice also had higher expression of PPARα target genes in the liver and white adipose tissue (WAT) and of thermogenic genes in the WAT than adult SO-m mice, whereas adult OFO-f and SO-f mice did not differ. We conclude that uterine PPARα activation caused by maternal OFO ingestion affects hepatic PPARα activity and adipose thermogenic capacity and contributes to the differential susceptibility to DIO in the male and female offspring in adulthood.

Abbreviations

BAT
brown adipose tissue
DIO
diet-induced obesity
E
embryonic day
NEFA
nonesterified fatty acid
OFO
oxidized frying oil
OFO-f
female offspring of dams fed the oxidized frying oil diet
OFO-m
male offspring of dams fed the oxidized frying oil diet
SO
fresh soybean oil
SO-f
female offspring of dams fed the fresh soybean oil diet
SO-m
male offspring of dams fed the fresh soybean oil diet
WAT
white adipose tissue

Cited by (0)

2

Author disclosures: H.-C. Chuang, C.-F. Huang, Y.-C. Chang, Y.-S. Lin, and P.-M. Chao, no conflicts of interest.