Changes in Spontaneous Working-memory, Memory-recall and Approach-avoidance following " Low Dose " Monosodium Glutamate in Mice

The study investigated the effects of 'low doses' of monosodium glutamate (MSG) on hippocampal-related (spontaneous working-memory, memory-recall and anxiety) behaviours, and hippocampal glutamate/glutamine levels. A two-trial Y-maze test and 8-arm radial-arm maze spontaneous working-memory test were used to assess the effects of acute and repeated administration of MSG, on novel-arm choice on retrial and spatial working-memory; while anxiety-related behaviors were assessed in the elevated plus maze. In the elevated plus maze, radial-arm maze and Y-maze, MSG administration was associated with significant anxiolytic and memory-enhancing effects at 10 mg/kg (after both acute and repeated dosing); however, higher doses used in this study were associated with significant anxiogenesis and memory retardation. Hippocampal glutamate and glutamine levels did not increase significantly at any of the doses of MSG. In conclusion, MSG administration at low doses was associated with significant changes in hippocampal-dependent behaviours without a concomitant significant shift in hippocampal glutamate/glutamine levels.


Introduction
The mammalian central nervous system produces and modulates behaviour through cycles of complex interactions involving neurotransmitters and their receptors.Its primary excitatory neurotransmitter, glutamate, is important in synaptic plasticity, learning and development [1], and has been implicated in the pathogenesis of learning, memory and anxiety disorders [2,3].Interaction of glutamate at the N-Methyl-D-Aspartate (NMDA) receptors forms the basis for long-term potentiation/depression in memory processes.Glutamate acts as a neurotransmitter at most of the excitatory synapses in the central nervous system, and glutamatergic neurons are widely distributed in the forebrain [4,5] and hippocampus [5,6].
Glutamate is derivable from exogenous sources, such as dietary monosodium glutamate (MSG), a culinary flavour-enhancer.MSG is metabolised to yield free glutamate, which is not biochemically different from the endogenous ligand, and studies have shown that MSG administration results in reproducible behavioural changes in rodents [7,8].Studies have shown that daily MSG consumption in human averages about 0.3-1.0g [9], although there are variations amongst different nationalities all over the world.MSG consumption ranges from 0.5g/day in America [10] to 0.3-0.5 mg/day amongst the Europeans.In Asia, average consumption ranges from between 1.2-1.7 g/day [11)] in Korea and Japan, to 3-3.8 g/day in Taiwan and China [12].Amongst the Thai population, MSG consumption ranges between 0.4-14 g/day, with an average daily intake of 6 g [13].Advances in food-production practices worldwide and the need to improve the taste of food, in recent times, could mean that the permissible levels of consumption may be significantly exceeded within short periods of time.Hence, there is the need to study not only the morphological changes associated with MSG, but also its effects on hippocampal-dependent behaviours.
A number of studies have sought to explore the relationship between MSG ingestion (at increasing doses) and memory/anxiety, with diverse results.Available data show that ingestion of MSG at extremely high doses (levels significantly higher than average daily human consumption) led to a decrease in spatial-memory and learning tasks performance in adult/neonatal rats or mice [1,7,[14][15][16][17].The deficits in spatial memory and learning in MSG-treated mice have several possible explanations.A deficiency of NMDA glutamate receptors in the hippocampus, degenerative retinal function/impaired vision, cholinergic deficiency that leads to reduced acetylcholine synthesis (especially in the hippocampus), or a combination of some or all of these performance inhibitors are possible explanations [1,17].Studies have also demonstrated that MSG, at very high doses was anxiogenic [18]; this is notable, as imbalances in glutamate/gamma-amino-butyric acid ratio have been implicated in anxiety disorders.
Interactions of glutamate with its ionotropic (mainly NMDA) receptors have sometimes been found to lead to neurotoxic changes, due to glutamate's effect on calcium availability at the neurons [19].Excessive activation of glutamate receptors is believed to contribute to neuronal injury, as persistent or overwhelming activation of glutamate-gated ion channels can result in degeneration of neurons, either through necrosis or apoptosis [20,21].This phenomenon called ''excitotoxicity,'' has been linked to the final common pathway of neuronal death in a number of disorders including Huntington disease, Alzheimer disease, amyotrophic lateral sclerosis (ALS), and stroke [19].
Early studies had described anatomical changes in specific brain regions secondary to MSG administration [22].Some of these studies also showed that the hippocampus is one of the most susceptible regions of the brain, and that of several animal species; mice were the most susceptible to MSG neurotoxicity [23].Hence, it is obvious from earlier studies using MSG that ingestion of high doses led to obvious anatomical changes in the hippocampi of susceptible species, in addition to behavioural changes.In a more recent study by us, repeated administration of MSG at the same doses used in the present study (equivalent to 0.7 g-5.6 g/day in a 70 kg man; doses within and below average daily consumption levels) was observed to cause hippocampal injury in mice, at the upper limit of dosing [8].The justification for this study therefore was the need to evaluate the effects of repeated administration of MSG (at doses chosen with reference to previous studies [8,[24][25][26] on hippocampal-dependent (recognition memory in the Y-maze, spatial memory in the Y and radial-arm mazes, and anxiety test in the elevated-plus maze) behaviours; and to compare effects seen, with standard reference drugs (scopolamine and diazepam respectively).Our hypothesis is that repeated administration of MSG at concentrations currently associated with typical dietary consumption may significantly alter recognition-memory, working-memory and anxiety related behaviours.

Animals
Adult

Behavioural testing
Neurobehavioural tests were carried out in a quiet room between the hours of 8 a.m. and 2 p.m.
On each of the test days, animals were transported to the testing room in their home cages, and allowed to acclimatize for 30 minutes before behavioural tests, after which drug or vehicle was administered.Behavioural tests were conducted after 30 minutes of administration of MSG, based on the pharmacokinetics of oral MSG.At the onset of the tests, each animal was placed in the apparatus and its behaviour videotaped for subsequent analysis.After testing, each mouse was removed from the maze, returned to its home cage, the maze cleaned thoroughly with 70 % ethanol, and then wiped dry to remove any trace of odour.

Learning and memory test (Y-Maze, Radial-arm maze)
Hippocampus-dependent Y-maze task characterizes spatial recognition-memory, which is based on the propensity of rodents to gravitate toward novel spatial environments, which is not contingent upon either reward, or water-escape associated stress.The two-trial Y-maze test consisted of two trials separated by an inter-trial interval of 30 minutes to assess spatial recognition-memory.At the start of each session, each animal was gently placed at the end of the start arm, facing away from the central platform.The number of entries and time spent in each arm of the maze by each mouse were recorded over the trial session.Arm entry is defined as the entry of all four paws into one arm.For the first trial, mice were placed inside the start arm, while the novel arm is closed with a wooden block (the same arm was closed for all animals in all test groups).Therefore, mice were able to explore the start and other arm, but not the novel arm [28,29].Memory-retrieval (second trial) was evaluated in a test session carried out 30 minutes after the first trial [30].For this trial, trained animals were placed back in the maze in the same starting arm, with free access to all three arms.The Y-maze was also used as a measure of spatial working-memory, measured using the spontaneous alternation.Spontaneous alternation was measured using a Y-maze made up of three equally spaced arms (120°, 41 cm long and 15 cm high).Each mouse was placed in one of the arm compartments and allowed to move freely until its tail completely entered another arm.An alternation was defined as entry into all three arms consecutively [31].The number of actual alternations is number of sequential arm entries into three arms, designated A, B and C. The percentage alternation is calculated as {(Actual alternations/Total arm entry minus two) × 100} in a 5 minute interval.
Working-memory in the radial-arm maze was measured as alternation index, which is the ratio of sequential arm entries before error and total arm entry.The apparatus is made up of eight equidistantly spaced arms, each about 33 cm long, all radiating from a small circular central platform.
Working-memory was assessed when the rat enters each arm a single time over a 5 minute period.
Re-entry into the arms would result in a working-memory error [31].

Assessment of hippocampal glutamate and glutamine levels
At the end of the experimental period, animals were euthanized using diethyl-ether anesthesia and perfused transcardially with ice-cold saline.Whole brains were dissected out and the hippocampus sectioned and blotted dry.A 10 % hippocampal homogenate was prepared with ice-cold phosphate buffered saline using Teflon-glass homogenizer.The homogenate was centrifuged at 5,000 rpm (at 4 degrees Celsius), for 15 min.and the pellet discarded.The supernatant was used to assay brain glutamate and glutamine levels [8].

Plasma and brain glutamate and glutamine assay
Plasma and hippocampal glutamate and glutamine levels were assayed using glutamate and glutamine assay kit following the manufacturer's instructions.The glutamate assay kit provides a sensitive detection method of the glutamate in a variety of samples.The glutamate enzyme mix recognizes glutamate as a specific substrate leading to proportional colour development.The glutamine assay is based on the hydrolysis of glutamine to glutamate which produces a stable signal, which is directly proportional to the quantity of glutamine in the sample [8].

Statistical analysis
Data was analysed using Chris Rorden's ezANOVA for windows, version 0.98.Hypothesis testing was performed using analysis of variance (ANOVA).We tested the hypothesis, that acute and repeated administration of low doses of MSG causes significant changes in hippocampal-dependent behaviours, using a two-way analysis of variance (ANOVA).One way ANOVA was used for analysis of body weight, plasma and hippocampal glutamate and glutamine level data.Tukey highly significant difference (HSD) test was used for pairwise comparisons.Results are expressed as mean ± S.E.M, p values less than 0.05 were considered statistically significant.

Effects of monosodium glutamate on radial arm-maze spatial working-memory tasks
Figure 5 shows the effect of MSG on spatial working-memory in the radial arm-maze, measured as alternation index/5 minute, which is a fraction of number of correct alternation before first error and total number of alternations made in a five minute period.Two-factor ANOVA assessing the effect of two factors (MSG dose and duration of administration) revealed a significant effect of MSG dose (F (5,108) = 6.70, p < 0.010) and duration of administration (F (1,108) = 5.32, p < 0.020), with significant interactions between MSG dose x duration of administration (F (5, 108) = 3.88, p < 0.002).Pairwise comparisons of the effect of SCOP or MSG dose and vehicle (control) revealed a significant decrease in spatial memory with SCOP (p < 0.012, p < 0.001)) and at 80 mg/kg (p < 0.021, p < 0.001) of MSG, while at 10 mg/kg (p < 0.001, p < 0.001), a significant increase was seen following both acute and repeated administration respectively.Compared to SCOP however, a significant increase in spatial memory was seen at 10 (p < 0.001, p < 0.001), 20 (p < 0.013, p < 0.001) and 40 mg/kg (p < 0.001, p < 0.001) following acute and repeated administration respectively.Pairwise comparisons of the effect of acute and repeated dosing revealed no significant difference at any of the doses of MSG.

Effect of monosodium glutamate on locomotor activity
Table 2 shows the effects of MSG on locomotor activity in the Y-maze and radial arm maze respectively following 5 minutes of exploration.Following exploration in the Y-maze, two-factor ANOVA revealed a significant effect of MSG dose (F (5, 108) = 33.5, p < 0.001) and duration of administration (F (1, 108) = 74.44,p < 0.001) with no significant interaction between MSG dose × duration of administration (F (5, 108) = 9.45, p < 0.212).Pairwise comparisons of the effect of SCOP or MSG dose and vehicle following acute and repeated administration revealed a significant increase in locomotor activity with SCOP (p < 0.001, 0.021) and no significant difference at any of the doses of MSG respectively.Compared to SCOP, there was a significant decrease in locomotor activity at 10 (p < 0.002, p < 0.001), 20 mg/kg (p < 0.012, p < 0.031), 40 (p < 0.001, p < 0.001) and 80 (p < 0.001, p < 0.001)] of MSG following acute and repeated administration respectively.Comparison of the effect of acute and repeated dosing revealed no significant difference in locomotor activity at any of the doses of MSG.
Following exploration in the radial-arm maze, two-factor ANOVA revealed a significant effect of MSG dose (F (5, 108) = 321, p < 0.001) and duration of administration (F (1, 108) = 334.8,p < 0.001), with no significant interactions between MSG dose × duration of administration (F (5, 108) = 1.25, p < 0.402).Pairwise comparisons of the effect of SCOP or MSG dose and vehicle revealed a significant increase in locomotor activity with SCOP (p < 0.001, p < 0.001) but no significant difference at either dose of MSG following acute and repeated administration respectively.In comparison to SCOP, there was a significant decrease in locomotor activity at 10 (p < 0.001; p < 0.001), 20 mg/kg (p < 0.001; p < 0.001), 40 (p < 0.001; p < 0.001) and 80 mg/kg (p < 0.001; p < 0.001) of MSG following acute and repeated administration respectively.Comparisons of the effect of acute and repeated dosing revealed no significant difference in locomotor activity at any of the doses of MSG.

Effects of monosodium glutamate on plasma and hippocampal levels of glutamate and glutamine
Table 3 shows the effects of MSG on plasma and hippocampal levels of glutamate and glutamine at day 21.There was a significant increase in plasma glutamate (F (5, 45) = 16.52,

Discussion
Our study in mice (using test groups outlined in Table1) revealed that acute and repeated administration of MSG was associated with: (1) no significant effect on body weight (2) enhancement of spatial recognition and spatial working-memory at 10 mg/kg while impairing both at higher doses (3) mixed anxiety-related response, (4) an increase in plasma glutamate and glutamine levels at high doses, while only a non-significant trend towards an increase in hippocampal levels was seen.
In this study, administration of MSG did not significantly alter body weight, compared to vehicle (figure 1).This corroborates an earlier study in which similar results were reported [8], although reports on the effects of MSG consumption on body weight continue to be conflicting, with more studies supporting an age-related effect on body weight.MSG effects on body weight have been reported to be mediated by gut L-glutamate receptors which are connected to the afferent fibres of the vagus nerve or via L-In this study, MSG increased spatial-recognition (figure 2 and figure 3) and spatial workingmemory (figure 4 and figure 5) at 10 mg/kg and impaired it at higher doses, following both acute and repeated administration.Learning and memory in the Y-maze and radial-arm maze are measured by motor reactions; and the results of Y-maze and radial-arm maze locomotor activity (Table 2) showed a non-significant trend towards increased locomotion, which affirms that memory changes seen in this study were not simply a consequence of motor impairment.The effect seen at 10 mg/kg is suggestive of a possible nootropic effect; although, this contravenes the results of a number of studies in which MSG was administered at extremely high doses (doses that far exceed average daily human consumption), where impaired memory [15,[41][42][43] was reported irrespective of route of administration.In a previous study [44], acute administration of very low doses of MSG (0.5-1.5 mg/kg) showed no significant effect on spatial working-memory in the Y-maze.This also suggests that the possible nootropic effect of MSG is only observable within a narrow margin of doses.The possible mechanisms for this effect are still being studied.Studies have shown that the memory impairment seen with MSG are either as a result of interference with glutamate synthesis in the hippocampus [41,42], or through inhibition of the cholinergic system [41].A number of the glutamate taste receptors (Umami) [33][34][35][36].MSG administered by gavage delivers a loading dose of MSG to the gastrointestinal tract lumen, activating vagal afferents [36,37], indirectly through production and release of either nitric oxide and/or serotonin [38]; although MSG-mediated gastric response is concentration-dependent [34], which may account for the variations in body weight effects reported in a number of studies.Studies in which MSG was administered to neonatal or prenatal rodents reported increased weight gain at times bordering on obesity [39], whilst studies in which adults rats or mice were used either reported no weight change or a reduction [8,38,40].In this study, food and water intake (not reported) did not differ significantly from control animals, which suggests that the mechanisms responsible for lesser or no weight gain may be associated with higher energy expenditure rather than increased energy intake.
studies in which MSG has been associated with memory loss or no improvement in memory have either used very low doses [44] or very high doses [41].At the high doses, memory impairment has also been associated with neuronal injury, possibly due to glutamate excitotoxicity or oxidative stress [8,45].In a recently published study with doses similar to those used in this study, neuronal injury was not evident at doses less than 40 mg/kg [8], suggesting that at these levels, intensity of glutamate receptor stimulation is not high enough to causes excitotoxicity, although it is sufficient for memory enhancement.The results of hippocampal glutamate and glutamine levels (Table 3) in this study showed non-significant increases compared to vehicle.Recent experimental evidence supports the notion that 'umami' taste in the gastrointestinal tract can stimulate cortical and subcortical brain areas that are linked to working-memory [46].Using functional magnetic resonance imaging, Meyer-Gerspach et al. [46] found that MSG administration via nasogastric tube in human subjects was associated with stimulation of neuron clusters in the pre-central gyrus, post-central gyrus, hemispheric operculum, precuneus and the cingulate gyrus; however this did not result in significant changes in working-memory performance [46].It must be noted that a major limitation of their study is that MSG delivery through nasogastric tube The impairment in working-memory seen at the higher doses of MSG could be secondary to a combined effect of slight elevation of glutamate and glutamine, coupled with increased oxidative stress that has been reported at these two doses [8].The results of locomotor activity in this study showed no significant difference from vehicle.This implies that the memory-impairment seen at high doses were not locomotor/motor-related.
bypasses the physiological route of nutrient intake, as a result, information from the tongue and the gut are not integrated; this failure of integration is likely to have reduced the strength of stimulatory impulses delivered to the brain.In contrast, MSG was administered by oral gavage in our study.
In terms of practical usefulness, the application of glutamic acid as a nootropic agent is a topic that has generated lots of debates; presently, it is used as an adjunctive memory-enhancing agent, however, its usage is still neither popular nor backed by convincing experimental evidences.
In the EPM, (figure 6 and figure 7) acute MSG administration led to an anxiolytic response at the lower doses (10 and 20 mg/kg) and an anxiogenic response at the higher doses, when compared to vehicle.However, the anxiolytic response seen at lower doses of MSG is not comparable to that of diazepam.Repeated administration of MSG resulted in an anxiogenic response across all doses tested in comparison to both vehicle and diazepam controls.The results seen with repeated administration in this study corroborates a number of studies that have demonstrated anxiety behaviour with MSG administration [18,47].Increased glutamatergic transmission has been associated with anxiety disorders [48,49], and in a few studies, a correlation between increase in glutamate concentration in the hippocampus and/or amygdala and anxiety disorders in humans have been documented [50,51].Glutamate-GABA imbalances have also been implicated in anxiety disorders and the response elicited following oral MSG could mean that administration of MSG might have resulted in an alteration in the glutamate-GABA balance in the brain that is enough to cause the observed effects.The loss of an anxiolytic and assumption of anxiogenic response with continuous administration of MSG suggests that even at low doses, repeated exposure of the brain to MSG may alter anxiety-related behaviours.

Conclusion
In conclusion, MSG at doses studied is nootropic at low doses in mice, suggesting a need for further studies.Although, the association of MSG with memory loss and anxiogenesis at higher doses still suggest that continued caution in its use is necessary.

Conflict of Interest
Authors no conflicts of interest in this paper.
Choice of novel arm and time spent in the novel arm were then scored.The entire task is based on the innate explorative behaviour of rodents.If the animals have good recognition-memory, they will spend more time in the novel arm relative to the previously explored arms.Number of entries, and time spent in each arm was scored.Percentage time spent in the novel arm was scored as: time spent in the novel arm relative to the average time × by 100.Novel arm choice on retrial was scored as the number of times animals in the group entered the novel arm first/number of animals in the group × 100.
-related behaviours was measured in the elevated plus maze {EPM}.The EPM is plus-shaped, with two open arms measuring 25 × 5 × 5 cm lying across from each other and perpendicular to two closed arms measuring 25 × 5 × 16 cm with a centre platform (5 × 5 × 0.5 cm).The closed arms are enclosed by 2 high walls (16 cm) while the open arms have no side wall.Animals are placed in the central platform facing the open arm and behaviours recorded for 5 minutes.The criterion for arm visit was considered only when the animal decisively moved all its four limbs into an arm.The following measures were measured: Percent open arm entries (open arm entries/total arm entry multiplied by 100) and percent time spent in the open arms (time in open arms/ total time spent in the maze multiplied by 100) [32].Administration of an anxiolytic drug e.g.(diazepam) in rodents significantly increased the percentage of entries into, and time spent in the open arms.

Figure 1
Figure 1 represents the percentage body weight change, defined as the percentage difference between the final and initial body weights, divided by the initial body weight, multiplied by 100.There was no significant (F (4, 45) = 1.56, p < 0.786) difference in % weight increase at any of the doses compared to either vehicle or MSG.

Figure
Figure 1． Effect of repeated administration of monosodium glutamate on body weight.Each bar represents mean ± S.E.M, number of mice per treatment group = 10; VEH: Vehicle, mg/kg, p.o: milligram/kilogram body weight, per oral.

Figure 2
Figure 2 shows one way ANOVA analysis the effect of acute or repeated administration of MSG on novel arm choice on retrial in the 2-trial Y-maze test.Acute (F (5, 48) = 23.27,p < 0.015) and repeated (F (5, 48) = 12.10, p < 0.001) administration of MSG resulted in a significant increase in recognition-memory (measured as choice of the novel arm on retrial) at 10 mg/kg and a decrease at 20, 40 and 80 mg/kg, compared to vehicle respectively; compared to scopolamine however, there was an increase at 10, 20 and 40 mg/kg with acute administration, while with repeated administration significant increase was seen at 10, 20 and 40 mg/kg and a decrease at 80 mg/kg.

Figure
Figure 2． Effects of acute and repeated administration of MSG on novel arm choice on retrial.Each bar represents Mean ± S.E.M, Differences between experimental groups (MSG or SCOP) and control (VEH) was marked by asterisk (post hoc test: p < 0.05).Differences between dosages of MSG, VEH and SCOP are marked by hash (post hoc test; p < 0.05).Number of mice per treatment group = 10; VEH: Vehicle, MSG: monosodium glutamate, SCOP: scopolamine, mg/kg, p.o: milligram/kilogram body weight, per oral.

Figure 3
Figure 3 shows the effect of MSG on time spent in the novel arm on retrial.Two-factor ANOVA assessing the effects of two main factors (MSG dose and duration of administration) revealed a significant effect of MSG dose (F (5,108) = 456, p < 0.001) and duration of administration (F (1,108) = 5.88, p < 0.020), with significant interactions between MSG dose × duration of administration (F (5, 108) = 2.64, p < 0.030).Pairwise comparisons of the effects of scopolamine (SCOP) or MSG dose and vehicle (control) following acute and repeated administration revealed a

Figure
Figure 3． Effects acute and repeated administration of MSG on time spent in the novel arm on retrial.Each bar represents Mean ± S.E.M, Differences between experimental groups (MSG or SCOP) and control (VEH) was marked by asterisk (post hoc test: p < 0.05).Differences between dosages of MSG, VEH and SCOP are marked by hash (post hoc test; p < 0.05).Number of mice per treatment group = 10; VEH: Vehicle, MSG: monosodium glutamate, SCOP: scopolamine, mg/kg, p.o: milligram/kilogram body weight, per oral.

Figure 4
Figure 4 shows the effect of MSG on spatial working-memory in the Y-maze.Two-factor ANOVA assessing the effect of two factors (MSG dose and duration of administration) revealed a significant effect of MSG dose (F (5,108) = 23.1, p < 0.001) and duration of administration (F (1,108) = 11.20,p < 0.003), with a significant interaction between MSG dose × duration of administration (F (5,108) = 23.90, p < 0.001).Pairwise comparisons of the effect of SCOP or MSG doses and vehicle (control) following acute administration, revealed a significant decrease in spatial

Figure 4．
Figure 4． Effects of acute and repeated administration of MSG on Y-maze spatial working-memory.Each bar represents Mean ± S.E.M, Differences between experimental groups (MSG or SCOP) and control (VEH) was marked by asterisk (post hoc test: p < 0.05).Differences between dosages of MSG, VEH and SCOP are marked by hash (post hoc test; p < 0.05).Number of mice per treatment group = 10; VEH: Vehicle, MSG: monosodium glutamate, SCOP: scopolamine, mg/kg, p.o: milligram/kilogram body weight, per oral.

Figure 5．
Figure 5． Effects of acute and repeated administration of MSG on radial-arm maze spatial working-memory.Each bar represents Mean ± S.E.M, Differences between experimental groups (MSG or SCOP) and control (VEH) was marked by asterisk (post hoc test: p < 0.05).Differences between dosages of MSG, VEH and SCOP are marked by hash (post hoc test; p < 0.05).Number of mice per treatment group = 10; VEH: Vehicle, MSG: monosodium glutamate, SCOP: scopolamine, mg/kg, p.o: milligram/kilogram body weight, per oral.

Figure 6
Figure 6 shows the effect of MSG on % open-arm entries in the elevated plus maze.Two-factor ANOVA assessing the effect of two factors (MSG dose and duration of administration) revealed a significant effect of MSG dose (F (5, 108) = 9.40, p < 0.001) and duration of administration (F (1, 108) = 5.38, p < 0.002), with no significant interaction between MSG dose × duration of administration (F (5, 108) = 0.85, p < 0.342).Pairwise comparisons of the effect of diazepam (DIZ) or MSG dose and vehicle (control) revealed a significant increase in open arms entry with DIZ (p < 0.001, p < 0.001), at 10 (p < 0.001, p < 0.001) and 20 mg/kg (p < 0.001, p < 0.001) of MSG, while at 80 mg/kg (p < 0.001, p < 0.001), a significant decrease was seen following acute and repeated administration respectively.In comparison to DIZ however, there was a significant decrease in number of open arm entries at all doses of MSG (p < 0.001) following acute and repeated administration respectively.Comparisons of the effect of acute and repeated dosing revealed no significant difference in number of open arm entry at any of the doses of MSG with repeated compared to acute administration.

Figure 6．
Figure 6． Effects of acute and repeated administration of MSG on % open arm entry.Each bar represents Mean ± S.E.M, Differences between experimental groups (MSG or SCOP) and control (VEH) was marked by asterisk (post hoc test: p < 0.05).Differences between dosages of MSG, VEH and SCOP are marked by hash (post hoc test; p < 0.05).Number of mice per treatment group = 10; VEH: Vehicle, MSG: monosodium glutamate, SCOP: scopolamine, mg/kg, p.o: milligram/kilogram body weight, per oral.

Figure 7
Figure 7 shows the effect of MSG on % time spent in the open-arm of the EPM.Two-factor ANOVA assessing the effect of two factors (MSG dose and duration of administration) revealed a significant effect of MSG dose (F (5,108) = 12.20, p < 0.001) and duration of administration (F (1, 108) = 7.44, p < 0.010), with significant interactions between MSG dose × duration of administration (F (5,108) = 4.55, p < 0.002).Pairwise comparisons of the effect of DIZ or MSG dose and vehicle (control) revealed a significant increase in time spent in the open arms with DIZ (p < 0.001, p < 0.001), at 10 (p < 0.001, p < 0.015) and 20 mg/kg (p < 0.001, p < 0.001) of MSG, while at 40 (p < 0.001, p < 0.001) and 80 mg/kg (p < 0.024, p < 0.013) a significant decrease was seen, following acute and repeated administration respectively.In comparison to DIZ, there was a significant decrease in time spent in the open arms at all doses of MSG (p < 0.050) following acute and repeated administration.Pairwise comparisons of the effect of acute and repeated dosing revealed no significant difference in time spent in the open arms at any of the doses of MSG with repeated compared to acute administration.

Figure 7．
Figure 7． Effects of acute and repeated administration of MSG on percentage time spent in the open arm.Effects acute and repeated administration of MSG on time spent in the novel arm on retrial.Each bar represents Mean ± S.E.M, Differences between experimental groups (MSG or SCOP) and control (VEH) was marked by asterisk (post hoc test: p < 0.05).Differences between dosages of MSG, VEH and SCOP are marked by hash (post hoc test; p < 0.05).Number of mice per treatment group = 10; VEH: Vehicle, MSG: monosodium glutamate, SCOP: scopolamine, mg/kg, p.o: milligram/kilogram body weight, per oral.

Table 1 . Experimental groups and number of animals in each test group (n). Groups Test Y-maze Radial-arm maze EPM VEH
Neuroscience and Behavioural Research" prepared by the Committee on Guidelines for the Use of Animals in Neuroscience and Behavioural Research; National Research council of the Carbohydrate) from weaning.Mice had free access to food and drinking water except during the behavioural tests.All procedures were conducted in accordance with the approved institutional protocols and within the provisions outlined in the "Guidelines for the Use of Animals in