Calpain inhibition as a possible new therapeutic target in multiple sclerosis

Multiple sclerosis (MS), the most common chronic autoimmune inflammatory disease of the central nervous system (CNS), is characterized by demyelination and neurodegeneration. In particular, neurodegeneration is a major factor in disease progression with neuronal death and irreversible axonal damage leading to disability. MS is manageable with current therapies that are directed towards immunomodulation but there are no available therapies for neuroprotection. The complex pathophysiology and heterogeneity of MS indicate that therapeutic agents should be directed to both the inflammatory and neurodegenerative arms of the disease. Activity of the Ca activated protease calpain has been previously implicated in progression of MS and its primary animal model, experimental autoimmune encephalomyelitis (EAE). The effects of calpain inhibitors in EAE involve downregulation of Th1/Th17 inflammatory responses and promotion of regulatory T cells, overall leading to decreased inflammatory cell infiltration in CNS tissues. Furthermore, analysis of brains, spinal cords and optic nerves from EAE animals revealed decreases in axon degeneration, motor neuron and retinal ganglion cell death. This resulted in improved severity of paralysis and preservation of visual function. Taken together, the studies presented in this brief review suggest that use of calpain inhibitors in combination with an immunomodulatory agent may be a potential therapeutic strategy for MS and optic neuritis.


Epidemiology of MS
Multiple Sclerosis (MS) is an autoimmune inflammatory and neurodegenerative disease of the central nervous system (CNS) that is the leading cause of neurologic disability in young adulthood, and there is no cure for the disease [1].The worldwide disease burden of MS is upwards of 2.5 million individuals, with an incidence of approximately 7 new cases per 100,000 people per year and a lifetime risk of 1 in 400.An estimated 400,000 cases have been diagnosed in the US with roughly 200 new cases every week.As many as 26% of MS patients live without impaired mobility in their lifetime, but a minority (15%) become rapidly disabled [2,3].The reduction in life expectancy due to MS is small, so most patients live at least 25 years after diagnosis, and the majority experience increasing neurologic disability for the duration of their lives.Estimates suggest that MS results in $9.5 billion in medical costs and lost productivity each year.
MS was first described nearly 200 years ago but many questions about causal factors, individual susceptibility and biomarkers of disease progression remain [4,5].Current treatments are directed towards immunomodulation but do not specifically target neurodegenerative processes.As such, therapies need to be developed that are directed towards this aspect of the disease, especially since damage to axons and neurons appears to predict long term disability in MS [4,[6][7][8].

Pathophysiology and Clinically Isolated Syndrome of Multiple Sclerosis
The current consensus is that MS pathology can be attributed to an inflammatory response subsequent to activation of auto-reactive T cells specific for myelin antigens.While the exact mechanisms of MS induction are still unknown, epitope mimicry and disturbed immune surveillance are the most well-studied theories of how infection may induce MS in susceptible individuals [9].Within the concept of epitope mimicry, one becomes infected by a virus or bacterium that expresses a protein with a sequence very similar to that of a CNS self-protein.The infection thus indirectly triggers an autoaggressive lymphocyte response that leads to progression of MS [10].There is also increasing evidence that the CNS is continuously surveyed by virus-specific T cells, which protect against reactivating neurotropic viruses.Acute viral infections may lead to the breakdown of self-tolerance in genetically predisposed individuals, resulting in reactivations of viruses in the CNS that induce the recruitment of both autoaggressive and virus-specific T cell subsets, causing relapses and cumulative disability [11].
The monitoring of a potential MS case begins when patients present with a Clinically Isolated Syndrome (CIS), which is a single neurologic attack compatible with MS.CIS commonly presents as difficulty walking, pain or numbness of the lower extremities, loss of central vision, double vision, bladder dysfunction, or cognitive deficits [3,12].Studies in CIS patients suggest that gray matter atrophy occurs very early in the MS disease course [13].Moreover, cerebral atrophy may even begin well before the clinical symptoms appear, and it may be independent of cortical demyelination.Klaver et al. found neurodegenerative changes in both normally myelinated normal-appearing gray matter (NAGM) and Type III MS lesions, however there was no significant difference in neuron and axon density between NAGM and Type III MS lesions [14].De Stefano demonstrated the presence of widespread, irreversible axonal damage independent of inflammatory lesions in the cerebral white matter at very early stages of the disease and despite heterogeneity of MS subtype [15].One pathological mechanism of gray matter atrophy is damage secondary to demyelination and axonal transection within an inflammatory lesion [16], but this mechanism alone is insufficient to explain why atrophy is at times scarce in the presence of inflammation and at times significant in regions with little or no active inflammatory demyelination [17,18].Thus, the task of elucidating the molecular mechanisms of the independent neurodegenerative process is at hand.

Optic neuritis
Optic neuritis (ON) is strongly associated with multiple sclerosis (MS) and is the first sign in the diagnosis of 15-20% of MS cases.Clinical electrophysiology and optical coherence tomography studies have demonstrated degeneration of the optic nerve and retina following an optic neuritis flare [19].Corollary animal studies have shown oligodendroglial death, myelin and axonal damage, and retinal ganglion cell (RGC) death [20].These events herald acute loss of visual acuity, contrast sensitivity, and visual field defects that are not fully reversible due to residual axon damage and neuronal loss [21][22][23][24].Changes to visual pathway structure in MS have been described as a window into the CNS, yielding valuable information about larger disease processes.
Current approved disease-modifying therapies (DMTs), based on their mechanisms of action, are limited in their ability to protect or repair damage to the optic nerve, brain and spinal cord.Addition of a neuroprotective agent to the DMT regimen may significantly delay disease progression and provide an option for patients who receive minimal benefit from DMT alone.
MS and ON have been well-studied in the animal model experimental autoimmune encephalomyelitis (EAE) [25][26][27].Increased expression and activity of calpain, a Ca 2+ -activated protease, has been detected in the CNS tissues of EAE rodents during disease exacerbations.Further study has revealed that activated calpain plays a role in promotion of inflammation, demyelination, and neurodegeneration in MS disease models [28][29][30].Calcium influx and calpain activation early in the disease course are hypothesized to contribute to neurological impairment in EAE and MS.Therefore, prophylactic or therapeutic treatment with calpain inhibitor may attenuate the events that lead to cell death and axonal damage in these diseases, ultimately improving neurological function.

Calpain
Among the proteolytic enzymes, calpain stands out as an important player in the pathogenesis of many CNS disorders, including traumatic brain injury [31], spinal cord injury [32], Parkinson's Disease and MS [33][34][35].Calpain, a Ca 2+ -activated cysteine protease, is a neutral and non-lysosomal proteolytic enzyme typically found in the cytosol of all animal cells.Inactive calpain is a pro-enzyme that is activated in the cytosol with an increase in intracellular free Ca 2+ concentrations.The pro-enzyme may also relocate to the membrane for activation in contact with membrane-bound phospholipids and then for degradation of membrane proteins.Fifteen genes within the human genome encode the calpain isoforms that form the calpain family [36].Some of the calpain isoforms are found in specific tissues.Only two major calpain isoforms, μ-calpain, and m-calpain, are expressed in all cell types.In this review "calpain" refers to the ubiquitous calpain isoforms, μ-calpain and m-calpain, which require 2-80 μM and 0.2-0.8mM Ca 2+ concentrations, respectively, for half-maximal activity in vitro.The μ-calpain and m-calpain isoforms exist as heterodimers consisting of a catalytic 80 kD large subunit and a regulatory 30 kD small subunit.Calpain exists as a pro-enzyme in the cytosol where the normal range of free Ca 2+ concentration is 50-100 nM in resting cells [37].An increase in the cytoplasmic free Ca 2+ concentration activates calpain, producing the active 76 kD fragment for proteolysis in the cells.At the normal range of intracellular free Ca 2+ concentrations, calpain is activated to perform important physiological functions such as cytoskeletal rearrangement, long-term potentiation, processing of hormones, and protein turnover [38].However, when intracellular free Ca 2+ concentrations exceed the normal threshold, uncontrolled and prolonged calpain activation may occur.An increased activation of ubiquitous calpain has been associated with processes integral to demyelination and neurodegeneration [31,39,40].
Both μ-calpain and m-calpain demonstrate the same substrate specificity.Cytoskeletal proteins, membrane proteins, cytokines, transcription factors, protein kinases, phosphatases, and lens proteins are preferred calpain substrates.Calpain substrates are proteolyzed in a limited manner rather than digested to smaller peptides, suggesting a modulation of their function [41].Calpastatin is an endogenous protein inhibitor that specifically inhibits ubiquitous calpain activity [36].The increase of the calpain/calpastatin ratio is a consequence of excessive activation of calpain that can degrade calpastatin either in a conservative or non-conservative way.Calpastatin is always considered a suicide substrate, but in normal conditions of calpain activity, this ratio, instead of increasing, decreases as conservative calpastatin digestion produces units retaining the ability to inhibit calpain [36].Calpastatin is not cell permeable, and therefore is not usable as a therapeutic option for inhibition of intracellular calpain activity.Extensive research has, however, produced different groups of cell-permeable calpain inhibitors, some of which appear to be promising for neuroprotection in animal models of CNS injuries and diseases such as acute viral encephalitis [42], stroke and Parkinson's Disease [43][44].

Calpeptin
Several commercially available calpain inhibitors are employed in experimental studies of MS.A challenging aspect of calpain inhibitor development has been to identify calpain-selective inhibitors, as many older generation agents also inhibit other cysteine proteases, serine proteases or the proteosome [45].Synthetic calpain inhibitors can be categorized into two major groups, the peptide and non-peptide inhibitors.The peptide inhibitors can be further classified as reversible (peptidyl aldehydes, peptidyl α-ketoamides) or irreversible inhibitors (peptidyl epoxides) [46].However, the peptide inhibitors have a common mechanism of action that involves formation of hemiacetal or theohemiacetals with active site cysteine or serine residues.Since µ and m-calpain are ubiquitously expressed and have diverse physiological functions, reversible inhibitors are preferred in order to avoid side effects secondary to permanent, non-specific blockage of calpain activity.Furthermore, the cellular permeability of these inhibitors has been enhanced via N-terminal capping and esterification of the carboxyl group with lipophilic groups [47,48].Peptidyl epoxides and peptidyl aldehydes in particular have greater selectivity for cysteine proteases vs. serine proteases [49,50].Calpeptin (EMD Biochemicals, Gibbstown, NJ) is a cell-permeable peptidyl aldehyde inhibitor of µ and m-calpain.Although any protease with active site cysteine residues (e.g.papain) may be targeted, calpeptin is highly substrate selective for calpains 1 and 2 (ID50 = 52 nM for calpain-1; ID50 = 34 nM for calpain-2; ID50 = 138 nM for papain).Calpeptin has been shown to prevent apoptosis of various cell types in vitro, including lympho cytes [51], cardio myocytes [52][53][54], primary oligodendrocytes [55], and retinal ganglion cells (RGCs) [56].Furthermore, calpeptin treatment can reduce the development of acute and chronic inflammation in vivo and attenuate signs of EAE paralysis [57,58].A limitation of calpeptin for in vivo use is its poor aqueous solubility, which necessitates intraperitoneal delivery of this agent after it has been dissolved in dimethyl sulfoxide (DMSO).

A role for calpain in inflammatory demyelinating disease
As mentioned previously, the hallmark features of MS and EAE are inflammation, demyelination, and neurodegeneration.In various experimental settings, calpain activity has been shown to play a role in all three pathophysiological events (Table 1).Calpain activity plays a key role in increasing the expression of many pro-inflammatory mediators (e.g.NF-κB, Cox-2) and participates in activation and migration of T cells [67][68][69][70][71]. Calpain released from activated T cells degrades myelin basic protein (MBP) and other myelin components in vitro and in a rat model of prenatal hypoxia-ischemia [72,73].Calpain activity has also been implicated in axonal damage and death of neurons and oligodendrocytes [35,39,74,75], at least partially through modulation of proteins involved in classical receptor and mitochondrial apoptotic pathways [76][77][78].Specifically, in a Lewis rat model of acute EAE [77], an increase in TUNEL-positive neurons and internucleosomal DNA fragmentation in EAE spinal cords suggested that neurons died by apoptosis on days 8-10 following EAE induction.Beginning on day 8 of EAE, increases in calpain expression in the spinal cord correlated with activation of pro-apoptotic proteases, which occurred prior to the appearance of paralysis.An increase in calcineurin expression and a decrease in phospho-Bad (p-Bad) suggested that Bad activation contributed to apoptosis during acute EAE.An increase in the Bax: Bcl-2 ratio and activation of caspase-9 indicated involvement of the mitochondria in apoptosis.Caspase-8 activation suggested induction of the death receptor-mediated pathway of apoptosis.Endoplasmic reticulum stress leading to caspase-3 activation was also observed, leading to the conclusion that multiple apoptotic pathways were activated in the spinal cord during acute EAE, and that activated calpain has a role in neuronal apoptosis during the development of acute EAE.To test the hypothesis that calpain inhibition can alleviate disability in EAE, two experimental models were utilized.First, treatment of actively immunized EAE Lewis rats with the calpain inhibitor calpeptin (50-250 microg/kg) dose-dependently ameliorated paralysis [77].In the second model, MBP-specific T cells were harvested from actively immunized SJL/J mice and were then incubated in the presence or absence of the calpain inhibitor SJA6017 prior to adoptive transfer (AT) of the T cells to naive SJL/J mice.EAE mice that received MBP-specific T cells incubated with SJA6017 before AT demonstrated a dose-dependent reduction in the degree of paralysis during relapses as well as a reduction in the frequency of relapses [57].In both models, calpain activity, astrocyte reactivity, loss of myelin, axonal damage, and neuronal and oligodendrocyte death were attenuated in the spinal cord with calpain inhibitor therapy.Thus, the advantage of calpain inhibitors in comparison to the majority of current therapeutic agents is that they target both neurodegenerative and inflammatory pathways of disease in EAE.In addition to experimental evidence of a role for calpain in inflammatory demyelinating disease, clinical data from MS patients is emerging.Expression and activity of calpain was increased in post-mortem brain tissue from MS patients and has been co-localized with glial and CD4+ T cells and damaged axons in MS plaques, which has also been seen in EAE [39,99,100].The activation of myelin-specific T cells is thought to be a major event in the development and progression of MS and its models.Production of pro-inflammatory Th1 cytokines (e.g.TNF, IL-2, and IFN) by CD4 + T cells increases during an MS exacerbation [101,102].In contrast, anti-inflammatory Th2 cytokines (e.g.IL-4, IL-10, IL-13) are predominant during disease remission [103][104][105].Th17 cells also contribute to autoimmune pathogenesis, and cytokines that promote their development and proliferation include IL-17, IL-23, and IL-6 [106][107][108].Th17 cells can efficiently cross the blood-brain barrier using alternate paths from Th1 cells, promote blood brain barrier disruption, and induce activation of other inflammatory cells in the CNS [109].Furthermore, increased numbers of peripheral blood mononuclear cells including CD4+ and CD8+ T cells have been shown to express high levels of IL-17 mRNA during MS relapses [107,110,111].On the other hand, dendritic cells (DCs) of MS patients and EAE mice express abnormally low levels of cytoplasmic indoleamine 2,3-dioxygenase (IDO) [112].IDO is a heme-containing enzyme that catalyzes the aerobic metabolism of L-tryptophan to N-formylkynurenine, which is the first and rate-limiting step in the kynurenine pathway.Initiation of the IDO-kynurenine pathway facilitates immune inhibitory function, and local DO-mediated tryptophan depletion leads to starvation and stress of Th1 cells, impaired function of bystander Th1 cells, and apoptosis [113][114][115][116]. Therefore, a high level of IDO expression may precede a favorable shift in Th1/Th2-mediated immune responses in chronic inflammatory diseases such as MS, and measurement of IDO gene expression and activity in the blood is being explored as a useful biomarker to monitor the course of relapsing-remiting MS [117,118].Higher levels of calpain are expressed in both unactivated and activated PBMCs from MS patients compared to controls, and inhibition of calpain in these cells attenuates secretion of IL-2 and IFN, which may promote an anti-inflammatory cytokine bias [89].Furthermore, calpain inhibition in primary myelin basic protein-specific T cell cultures increases Th2 proliferation and cytokine profile with relative decreases in Th1 and Th17 proliferation [66].Further investigation demonstrated that calpain inhibition downregulated several pro-inflammatory cytokines (IL-17, IL-23, TNF, G-CSF, IL-12) in MS PBMCs while it upregulated IDO expression and limited T cell proliferation, indicating that inhibition of calpain may ameliorate immune pathology in MS [119].

The efficacy of calpain inhibition in optic neuritis
The optic nerve serves as a marker of neurodegeneration for other CNS tissues in MS and EAE [120].The visual dysfunction that accompanies ON is a common cause of disability and reduced quality of life in MS [121,122].Intravenous methylprednisolone (MP) is currently the drug of choice to hasten visual recovery and reduce the risk of MS during an acute episode of ON.However, MP fails to improve long-term visual disability [22,123].A prospective cohort study of EAE mice revealed that disruption of the blood-optic nerve barrier (BONB) began as early as 3 days post-immunization [77], suggesting that ON may be an early event during EAE progression.As observed in MS patients, EAE-ON animals also demonstrate pathological changes in visual evoked potential (VEP) and electroretinogram (ERG) recordings, which reflect damage to the optic nerve and retina, respectively [26,67,124].Increased expression and activity of µ-calpain and m-calpain has been detected in EAE-ON optic nerves prior to paralysis onset [125,126].Moreover, treatment with EAE rats with calpeptin reduced the level of active calpain expression and attenuated inflammatory marker expression (iNOS, COX-2, NF-kB), microgliosis, astrogliosis, and expression of aquaporin 4 (AQP4).The Bax: Bcl-2 ratio, production of tBid, PARP-1, expression and activities of pro-apoptotic caspases, and internucleosomal DNA fragmentation were also attenuated in the optic nerves of treated rats [126].In a related EAE study, calpeptin was shown to prevent apoptosis of retinal ganglion cells (RGCs) by downregulating expression of pro-apoptotic proteins and the pro-inflammatory molecule nuclear factor-kappa B (NF-κB) [74,119,126].Furthermore, a rat model of EAE induced with myelin oligodendrocyte glycoprotein demonstrated that significant RGC loss precedes the onset of pathologically defined ON, and calpeptin attenuates loss of RGCs [35,127].In the study by Hoffman et al. [127], manganese-enhanced magnetic resonance imaging was used to monitor preclinical elevations in the retina and optic nerve of induced rats.Calcium elevation correlated with an increase in calpain activation during the induction phase of ON, as revealed by increased calpain-specific cleavage of spectrin.Calpeptin treatment reduced calpain activity and protected retinal ganglion cells from degeneration.These results further establish that elevation of retinal calcium levels and calpain activation are early events in ON, which make this pathway an ideal target for therapeutic intervention.
The neuroprotective efficacy of SNJ 1945, an inhibitor with superior oral bioavailability to calpeptin, has mainly been tested in mouse models of retinal disease.In a model of N-methyl D-aspartate (NMDA)-induced excitotoxic injury, two oral doses of SNJ 1945 significantly inhibited TUNEL+ cell death in the ganglion cell layer (GCL) and inner nuclear layer (INL) and prevented thinning of the inner photoreceptor layer (IPL).Furthermore, levels of cleaved spectrin fragments (surrogates of calpain activity) were increased as early as 6 h after NMDA injection, and spectrin cleavage was attenuated by SNJ 1945 [128].Similarly, oral dosing of SNJ 1945 prevented outer nuclear layer (ONL) atrophy in a mouse model of light-induced retinal degeneration [129].An in vivo mouse model of oxidative stress in RGCs showed that oxidative stress directly activated the calpain pathway and induced RGC death.Importantly, inhbition of the calpain pathway with SNJ 1945 was neuroprotective [130].In an ON treatment study, EAE was induced in B10.PL mice with myelin basic protein at Day 0, then the mice received twice daily oral dosing of SNJ 1945 from Day 9 until sacrifice on Day 26 [35].Throughout the study, visual function was determined by electroretinogram recordings and daily measurement of optokinetic responses (OKR) to a changing pattern stimulus.EAE mice manifested losses in OKR thresholds, a measurement of visual acuity, which began early in the disease course.There was a significant bias toward unilateral visual impairment among EAE-ON eyes.Treatment with SNJ 1945, initiated after the onset of OKR threshold decline, improved visual acuity and pattern electroretinogram amplitudes, with attenuation of RGC death.Furthermore, calpain inhibition spared oligodendrocytes, prevented degradation of axonal neurofilament protein, and attenuated reactive astrocytosis.The finding of early, unilateral visual impairment in this mouse model parallels the clinical presentation of ON.Overall, these findings suggest that administration of an orally bioavailable calpain inhibitor may preserve visual function in clinical ON.

Future Studies
Some of the in vitro studies described above can be enhanced by the introduction of calpain 1 or 2 gene silencing in certain cell types, which allow unequivocal testing of a role for calpain in immune and neurodegenerative processes [63].Use of silencing might aid the identification of a molecular mechanism for events such as upregulation of IDO.Moreover, a calpain heterozygous transgenic mouse and a calpastatin knockout mouse model are being used in related studies of neuroprotection and utilization of these animals could greatly enhance the understanding of EAE pathophysiology and provide a standard by which the efficacy of inhibitors can be compared [131][132][133].
Although the neuroprotective efficacy of calpain inhibitors has been established, their use in chronic demyelinating disease is limited by their ability to cross the blood-brain barrier.Fusion of calpain inhibitors with molecules that have specific transporters in the brain (e.g.dendrimers) may be an alternative strategy to achieve entry into the CNS that also allows dose-sparing [134,135].
Finally, an important theme that has emerged is the concept of pairing a neuroprotectant compound with an established disease-modifying therapy to treat EAE or MS.Data from a recent report indicates that natalizumab treatment can actually reduce the accumulation of nerve injury in relapsing-remitting multiple sclerosis, as assessed by release of NFL into the CSF [136].Likewise, in EAE, IFN-β-1a treatment three times per week slightly decreases the loss of RGCs.However, in contrast to neurotrophic factor effects, IFN-β-1a does not directly protect cultured RGCs from apoptosis.IFN-β-1a was found to be a suitable candidate to be combined with a directly neuroprotective agent such as a calpain inhibitor in order to further decrease axonal and neuronal degeneration in MS patients [137].

Conclusion
As re-iterated throughout this review, MS and ON have inflammatory, demyelinating, and neurodegenerative aspects that must be further elucidated and addressed therapeutically.More importantly, although patients experience disease relapses and remissions, gray matter damage is permanent and appears to be strongly associated with disability progression.Previous studies have highlighted the fact that known mechanisms of axon degeneration, namely, impaired axonal transport from the cell body, mitochondrial failure, and an increase in intra-axonal calcium, involve calpain-mediated degradation of axonal proteins [138].The studies presented in this review build a case that calpain inhibition, possibly in combination with an approved immunomodulatory agent, is a potential novel strategy for neuroprotection in MS.