Hybrid agarose gel for bone substitutes

Over the last decades, different materials have been investigated to overcome some flaws of bone substitutes. Even though various materials have been proposed for this conception, the in vivo assessments have still highlighted a lack of bioactivity and integration. In this context, this work focuses on the development of hybrid gel with surface properties specifically designed to promote bone regeneration by a sustained local delivery of active agents. We propose a new approach using modified-silica with high specific surface area and superior hydrophilicity dispersed in agarose hydrogel. In this optic, silica particles were dispersed in agarose solutions before the gelation of the composite upon cooling. The dispersion of the silica particles in the agarose gel was determined via scanning electronic microscopy. The degradation of the silica/agarose gels was also studied over a period of 12 weeks. Finally, the influence of the addition of silica on the permeability of the agarose gel was assessed via a diffusion test. The results showed that modified-silica particles exhibit a wide size distribution (500 nm and 10 μm) and can form clusters with higher size after their dispersion in agarose (up to 100 μm). The hybrid gel was stable over 12 weeks in aqueous solution. Moreover, no difference in permeability was noted between the hybrid gel and agarose hydrogel, allowing molecules up to 3 nm in diameter to diffuse freely within 1 mm thick agarose gels in less than 24 h. The present results indicate that hybrid agarose gel could represent an attractive matrix to disperse silica for scaffold applications.

Abstract: Over the last decades, different materials have been investigated to overcome some flaws of bone substitutes. Even though various materials have been proposed for this conception, the in vivo assessments have still highlighted a lack of bioactivity and integration. In this context, this work focuses on the development of hybrid gel with surface properties specifically designed to promote bone regeneration by a sustained local delivery of active agents. We propose a new approach using modified-silica with high specific surface area and superior hydrophilicity dispersed in agarose hydrogel. In this optic, silica particles were dispersed in agarose solutions before the gelation of the composite upon cooling. The dispersion of the silica particles in the agarose gel was determined via scanning electronic microscopy. The degradation of the silica/agarose gels was also studied over a period of 12 weeks. Finally, the influence of the addition of silica on the permeability of the agarose gel was assessed via a diffusion test. The results showed that modified-silica particles exhibit a wide size distribution (500 nm and 10 µm) and can form clusters with higher size after their dispersion in agarose (up to 100 µm). The hybrid gel was stable over 12 weeks in aqueous solution. Moreover, no difference in permeability was noted between the hybrid gel and agarose hydrogel, allowing molecules up to 3 nm in diameter to diffuse freely within 1 mm thick agarose gels in less than 24 h. The present results indicate that hybrid agarose gel could represent an attractive matrix to disperse silica for scaffold applications.
to promote a better dispersion of silica in the agarose matrix. This modified silica may exhibit high specific surface area, superior hydrophilicity, and less aggregates forms in comparison to non-modified silica [36,37]. This work includes the preparation of the hybrid gels using agarose and this modified-silica, as well as a study of their morphology and dissolution. Moreover, the permeability of the hybrid gel is analyzed via the diffusion of polyethylene glycol markers.

Silica gel synthesis
The synthesis is based on previous studies by our group [36,37]

Hybrid agarose gel preparation
Agarose gels without silica (denoted AG) and with 2 wt% of silica (denoted AG-Si) were prepared. This proportion of silica was selected based on previous works studying the diffusion of large biomolecules in silica-agarose gels for different applications such as chromatography or separation [38,39].
The silica particles were dispersed in water in an ultrasonic bath for 6 h to obtain a 4 wt% silica dispersion. The dispersion was then kept in a water bath at 60 ℃ until mixing. In parallel, agarose was dissolved in water at 95 ℃ for 30 min to obtain a 2 wt% solution of agarose. The agarose solution was then kept in a water bath at 60 ℃ for 30 min before mixing. The final solution was prepared by adding the same volume of the silica dispersion and the agarose solution in a vial dipping in the water bath at 60 ℃. The vials were then mixed by inversion. 2.4 mL (degradation and thickness tests) or 500 µL (permeability test) of the final solution were placed in a petri dish of 3.5 cm in diameter (degradation and thickness tests) or in an insert of 12 mm in diameter (permeability test). The solution was then let to cool down at room temperature for 30 min. Agarose gels without silica were also prepared using the same procedure, but replacing the silica dispersion by water. The obtained gels were directly characterized to avoid dehydration.

Sample characterization
The organic content was determined by thermogravimetric analysis (TGA, TGA 7 from Perkin-Elmer) under air atmosphere at a heating rate of 20 ℃/min between 50 and 90 ℃ and 10 ℃/min between 90 and 750 ℃. The weight loss was measured between 150 and 750 ℃.
The textural properties were characterized by nitrogen adsorption-desorption isotherms in an ASAP 2420 multi-sampler adsorption-desorption volumetric device from Micromeritics. The surface area was evaluated using Brunauer, Emmett, and Teller theory (S BET ) [40,41]. V P was defined as the specific liquid volume adsorbed at the saturation pressure of nitrogen. The mesoporous pore size distribution was determined by Barrett, Joyner, and Halenda theory [42,43].
The point of zero charge (PZC) was determined by the method of equilibrium pH at high loading [44]. The mass of the samples was first adjusted to obtain a surface loading equal to 10000 m 2 /L in 20 mL (in line with previous studies [44,45]). The porous solids were then soaked for 3 h under stirring in 20 mL of water solutions of various starting pH ranging from 1 to 13 adjusted using HCl or NaOH solutions. After equilibration, the equilibrium pH was measured using an InLab Expert Pro-ISM electrode from Mettler Toledo. The PZC of the solid corresponds to a plateau in a plot of the final pH vs. the initial pH [44].
The silica gel particle size was investigated via scanning electron microscopy (SEM, ESEM XL30 from Philips) with accelerating voltage of 20 kV.
The hybrid agarose gel thickness was measured using a micrometer (Mitutoyo, 293-344) after equilibration at 25 ℃ for 1 h. The measurements were performed on three samples (5 times per replicate).
The dispersion of silica gel in the agarose gel were analyzed via SEM. The hybrid agarose gel samples were dehydrated in a graded ethanol series, then infiltrated successively in 1,2-propylene oxide, 1:1 propylene oxide/epoxy resin mix and then in pure epoxy resin. The samples were placed in silicon molds and left to polymerize at 60 ℃ for 72 h. The resin blocks were then polished and imaged at an accelerating voltage of 30 kV (energy dispersive X-ray analysis, EDX), 20 kV (secondary electron analysis, SE), 10 kV (back scattered electron analysis, BSE) with a scanning electron microscope (SEM, ESEM XL30 from Philips). The EDX analysis provides elemental identification and quantitative compositional information. The SE analysis shows the topography of the surface. The BSE analysis is used to detect contrast between areas with different chemical compositions (i.e. resin/agarose vs silica). The SE analysis was first performed on the sample to detect the silica particles. The BSE and EDX analyses were then applied to confirm these observations.
The evolution of the macroscopic morphology of the hybrid agarose gels was followed by immersing the samples in 24 mL of PBS at 37 ℃ in closed vials. At scheduled times (i.e. 30 min, 4 h, 1 d, 1 week, 2 weeks, 4 weeks, 12 weeks), the samples were visually inspected and the pH of the supernatant was determined using an InLab Expert Pro-ISM electrode from Mettler Toledo. This analysis was performed in triplicates. As control, the evolution of the pH of a PBS solution was also followed.
The permeability of the hybrid agarose gels was assessed via a diffusion test. The inserts containing the gels were placed in wells of a 24-well cell culture plate containing 2.5 mL of mQ water. 500 µL of a 1 mgꞏmL −1 polyethylene glycol (PEG) solution containing PEG 400, 10000, and 35000 Da was placed on top of the gel. A schematic representation of the experimental set-up is presented in Figure S1. At scheduled times (i.e. 15 min, 30 min, 1 h, 4 h, 24 h), the solution in the well was removed, stored at −20 ℃ until analysis, and replaced by fresh mQ water. The PEG concentration was assessed via high performance liquid chromatography (HPLC) using PEG as standard. Chromatographic conditions were as follows: PL aquagel-OH mix 8 µm column (8 µm, 300 × 7.5 mm) followed by a PL aquagel-OH 308 µm column (8 µm, 300 × 7.5 mm), using milli-Q water as mobile phase at a flow rate of 1 mLꞏmin −1 (pump: T1-series 1050 from Hewlett-Packard) at 30 ℃ (oven: CHM from Waters) with an evaporation light scattering detector with a gas flow rate of 1.3 mLꞏmin −1 and a nebulization temperature of 40 ℃ (ELSD detector: 3300 ELSD from Alltech).

Silica gel
As shown in the previous studies by our group, the proportion of ethylene diamine groups in the silica is equivalent to 22 wt%, which is close to the theoretical calculated value (i.e. 22.7 wt%) and corresponds to a concentration of 2.05 mmolꞏg −1 [36,37]. Regarding the texture, the specific surface area S BET is equal to 330 m 2 ꞏg −1 and the total pore volume V P to 1.5 ± 0.1 cm 3 ꞏg −1 . The pore size distribution is characterized by the presence of a small peak at 8.8 nm and a larger peak at 18.7 nm. Finally, the point of zero charge (PZC) of the sample is 9.5. This means that the surface of the silica gel sample is positively charged at the pH of the morphology test (i.e. pH = 7.4), at agarose gel preparation (i.e. pH = 5), and at diffusion test (i.e. pH = 5).
The size and morphology of the silica particles is checked by SEM (Figure 1). Silica aggregates are present with a size ranging between 500-600 nm (encircled in red) and 10 µm (encircled in blue). These particles tend to agglomerate to form large structures with a size close to 50 µm.

Hybrid agarose gels
Hybrid agarose is a valuable alternative to improve the osseointegration of synthetic substitutes for bone reconstruction. The possibility to incorporate and release active agents, such as proteins, makes this strategy even more attractive.
When dealing with thermally sensitive biomolecules like proteins, both the process and the materials have to be selected to prevent any denaturation, notably regarding temperature. For this reason, during hybrid gel preparation, the silica suspension and the agarose solution should be kept at 60 ℃ for a minimal duration, even though the encapsulation of the biomolecule inside the silica could protect it [46,47]. Furthermore, to guarantee the biomolecule stability, the melting and gelation temperature can be decreased by playing on the concentration of the agarose solution, the content in methoxy groups naturally present in agarose, or the pretreatment of the agarose (e.g. alkali treatment, methylation, hydroxyethylation) [48,49].

Physical aspect
According to our formulation procedure, the resulting gels discloses a flat surface. The agarose gels without silica (AG) are transparent while the agarose gel containing 2 wt% silica (AG-Si) are translucent (Figure 4, T0). It is worth to mention that the gelation rate is not affected by the presence of silica, all being formed within about 30 min.
The syneresis of hybrid silica-agarose gel is also compared to agarose gel by studying their thickness after equilibration at 25 ℃ for 1 h. The thickness of the AG sample was 2.1 ± 0.2 mm (mean ± standard deviation) and the one of the AG-Si sample was 1.9 ± 0.1 mm. No significant difference was observed, indicating that the presence of silica does not affect the equilibration equilibrium of agarose.

Microscopic morphology
The SEM images of the hybrid gel are presented in Figure 2a-d. Three kinds of analysis were performed. First, the topography was analyzed via the SE analysis. Then, the BSE analysis was applied to detect contrast between areas with different chemical compositions (i.e. resin/agarose vs silica). Finally, the EDX analysis, which provides elemental identification and quantitative compositional information, was performed to detect the element present in each zone and determine if silica was present or not.
Contrary to the AG sample, which only exhibits a wave-like morphology, the AG-Si one presents agglomerates (indicated by a white arrow). As shown by the BSE analysis (Figure 2e-f), a difference in composition is observed between the agglomerates and the wave-like surface. This observation is confirmed by the EDX analysis ( Figure 3). The agglomerates contain silicon, showing the presence of silica. On the opposite, the wave-like regions are only composed of carbon, oxygen, and chlorine, indicating the presence of agarose (i.e. carbon and oxygen) and resin (i.e. carbon, oxygen, and chlorine). Silica is therefore located in these aggregates.   The large size of these agglomerates (10-20 µm wide and over 100 µm long) is probably due to the presence of larger silica particles, as observed in the previous section (diameter close to 10 µm). These results show that silica is not finely dispersed in the materials but rather agglomerates randomly distributed in the agarose matrix.
Even not studied for agarose, it has been shown that the hydrophilicity of nanofillers directly affect the filler-matrix interactions and subsequently the filler dispersion [50]. In order to avoid the formation of aggregates, the hydrophilicity of the silica could therefore be increased by modifying its surface with a larger quantity of ethylene diamine groups. In this case, increasing the hydrophilicity of the silica would enhance the interactions between the silica and the agarose gel (mainly composed of water), which would decrease the presence of silica aggregates. However, a higher number of ethylene diamine groups may disturb the pH medium as discussed later on.

Evolution of the macroscopic morphology
The macroscopic morphology of the gels in PBS (pH = 7.4) is followed during 12 weeks (Figure 4). The visual inspection does not show any evolution in the macroscopic aspect of the gels up to 12 weeks. The samples present a flat and smooth surface over the 12 weeks. Moreover, no change in color or transparency is observed. Another interesting result is the absence of syneresis (i.e. shrinkage of the gel due to the expulsion of the solvent). The pH of the supernatant is also followed over the 12-week period ( Figure 5). For the AG sample, the pH remains constant over time, close to the value of the PBS. On the opposite, a pH increase of the supernatant is observed over the first 7 d of the test for the AG-Si sample. After day 7, the pH remains stable around 7.9. This slight alkalinisation of the buffer probably results from the basicity of the ethylene diamine groups grafted on the modified silica. As already observed in previous studies by our group, the pH of a silica dispersion in pure water tends to reach an equilibrium value, i.e. point of zero charge = 9.5 in milli-Q water at a surface loading of 10000 m 2 ꞏL −1 [36,37]. In this case, due to the presence of a buffer and a lower surface loading (i.e. 700 m 2 ꞏL −1 ), this value is not reached. Nevertheless, these results indicate that silica particles are well accessible to the surrounding water giving rise to the protonation of ethylene diamine groups.
Interestingly, this pH increase does not influence the macroscopic morphology of the hybrid gel. Nevertheless, this observation should be taken into account for future development, notably regarding cell cytotoxicity and adhesion.

Permeability test
The permeability study is an important assay to evaluate the diffusion ability of different proteins through the hydrogels. Being hydrosoluble and devoid from ionic charge, PEG is used as model biomarker to assess the permeability and porosity properties of agarose gels [51]. Available in a very wide range of molecular weight, it allows to easily assess the diffusion ability of macromolecules in function of their hydrodynamic radii. In practice, we have selected 3 PEG with Mn of 400, 10000, and 35000 Da, corresponding to hydrodynamic radii of 0.4-0.5 nm, 2.8-3.3 nm and 5.7-6.6 nm, respectively [52,53].
Their diffusion kinetics profiles over 24 h are depicted in Figure 6. No significant difference is observed between the AG and AG-Si samples, indicating that the addition of silica does not significantly influence the diffusion of the PEG molecules through the samples. On the opposite, the kinetics of diffusion is directly influenced by the molar weight of the PEG. Indeed, the PEG 400 is detected in the well after 1 h while 24 h are required for the PEG 10000 and 35000 to be detected. Moreover, after 24 h, 70-75% of PEG 400 diffuses through the agarose gels, while only 15-20% of PEG 10000 and 5-6% of PEG 35000 diffuses. This decrease in diffusion rate with molar weight can be assigned to the increase in hydrodynamic diameter and molar weight of PEG according to the Ogston model (Eq 1) [54]. This model hypothesizes that the diffusion of solutes in hydrogel involves a series of random unit steps, which stop when the solutes encounter the hydrogel fiber. The polymer gel is considered as a random network composed of long and straight fibers, while the diffusing solute is extrapolated as a hard sphere.
where is the diffusion coefficient in the agarose gel (m 2 ꞏs −1 ); is the diffusion coefficient at infinite diffusion (in the range of 2-3 × 10 -10 m 2 ꞏs −1 for agarose gels); φ is the volume fraction of polymer fibers in the hydrogel (in the range of 0.1-0.3 for agarose gels); is the radius of the polymer fibers (1.9 × 10 −9 m for agarose), and is the radius of the solute (m). According to this model, increasing , while keeping , φ, and constant, lead to a decrease in the diffusion coefficient in the agarose gel . This decrease in diffusion coefficient with increasing molar weights has already been observed for various polymers diffusing through agarose [33,55]. In the case of PEG, the evolution of the diffusion coefficient with the molar weight follows an exponential relation (Eq 2) [51].
where is the diffusion coefficient in the agarose gel (m 2 ꞏs −1 ) and is the molar weight of PEG (Da). The diffusion coefficients calculated from Eq 2 are equal to 4.1 × 10 −10 m 2 ꞏs −1 for PEG 400, 7.5 × 10 −11 m 2 ꞏs −1 for PEG 10000, and 3.8 × 10 −11 m 2 ꞏs −1 for PEG 35000. These results show that molecules larger than 3 nm can diffuse in 1-mm thick agarose gels in less than 24 h. As an example, if we consider an active protein to bone repair like the growth factor Bone Morphogenetic Protein-2 that exhibits a hydrodynamic diameter around 3-7 nm [36,37], it can be anticipated that the hybrid gel is not a barrier to its diffusion. Hybrid gel could therefore be used as a matrix to depose silica particles loaded with active macromolecules at the surface of scaffolds, for instance.
Nevertheless, this hypothetical analysis should be viewed cautiously, as PEG mainly interacts with agarose through frictional effects (i.e. solvent effects, steric hindrance), while proteins could also interact through attractive or repulsive forces [51,56].

Conclusions
In conclusion, this work suggests an alternative technique for the deposition of silica particles on the surface of bone substitute scaffolds. The strategy relies on the dispersion of silica particles in an agarose solution before proceeding to its deposition at the scaffold surface by gelation of the agarose matrix.
In this optic, ethylene diamine-modified silica with a wide size distribution, ranging between 500 nm and 10 µm, is added in agarose solutions in order to prepare a hybrid gel. Once added to the agarose, silica particles agglomerate in clusters (10-µm wide and over 100 µm-large) randomly distributed in the matrix.
Although preliminary, the present results have shown that hybrid agarose gel could represent an attractive matrix to disperse silica to bone substitute scaffolds. Indeed, the hybrid gels are stable over a 12-week period. Moreover, the silica particles are accessible to the surrounding medium. Finally, the presence of silica does not affect the diffusion kinetics of PEG through the hybrid gel. It is also observed that PEG molecules larger than 3 nm could diffuse freely within 1 mm thick agarose gels in less than 24 h. Nevertheless, an increase in pH of the supernatant was noticed for sample containing silica, which can be assigned to the ethylene diamine functional groups grafted on silica particles. This observation should be taken into account for the in vitro evaluation, as a change in pH of the cell culture medium could affect cell viability.
In further studies, the deposition of this material at the surface of the scaffold should also be investigated, notably via casting or dip-coating.