The Role of Protein Engineering in Biomedical Applications of Mammalian Synthetic Biology
Open access
Date
2020-07-09Type
- Review Article
Abstract
Engineered proteins with enhanced or altered functionality, generated for example by mutation or domain fusion, are at the core of nearly all synthetic biology endeavors in the context of precision medicine, also known as personalized medicine. From designer receptors sensing elevated blood markers to effectors rerouting signaling pathways to synthetic transcription factors and the customized therapeutics they regulate, engineered proteins play a crucial role at every step of novel therapeutic approaches using synthetic biology. Here, recent developments in protein engineering aided by advances in directed evolution, de novo design, and machine learning are discussed. Building on clinical successes already achieved with chimeric antigen receptor (CAR‐) T cells and other cell‐based therapies, these developments are expected to further enhance the capabilities of mammalian synthetic biology in biomedical and other applications. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000426938Publication status
publishedExternal links
Journal / series
SmallVolume
Pages / Article No.
Publisher
WileySubject
bioengineering; cell‐based therapies; designer cells; synthetic biology; therapeuticOrganisational unit
03694 - Fussenegger, Martin / Fussenegger, Martin
Funding
785800 - Electrogenetics - Shaping Electrogenetic Interfaces for Closed-Loop Voltage-Controlled Gene Expression (EC)
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