Open Access

Enhanced expression of SOS1 is detected in prostate cancer epithelial cells from African-American men

  • Authors:
    • Olga A. Timofeeva
    • Xueping Zhang
    • Habtom W. Ressom
    • Rency S. Varghese
    • Bhaskar VS. Kallakury
    • Kan Wang
    • Youngmi Ji
    • Amrita Cheema
    • Mira Jung
    • Milton L. Brown
    • Johng S. Rhim
    • Anatoly Dritschilo
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijo_00000388
  • Pages: 751-760
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Abstract

African-American (AA) men experience an increased risk of developing prostate cancers as well as increased mortality following treatment as compared to European-American (EA) men. The aim of our study was to identify biological factors with the potential to predispose AA men to prostate tumor progression and metastasis. To identify cancer-specific gene expression patterns in AA men, we established primary prostate cancer epithelial cells from 14 AA and 13 EA men. High-throughput microarrays were used to investigate differences in global gene expression comparing the two groups. Quantitative RT-PCR and immunohistochemistry validated mRNA and protein expression levels. RNAi knockdowns provided support for biological significance for the identified genes in prostate cancer cells. Son of sevenless homolog 1 (SOS1) was overexpressed in AA male-derived primary prostate cancer epithelial cells. Depletion of SOS1 in PC3 and DU145 prostate cancer cells resulted in decreased capacities for cell proliferation, migration and invasion, at least partially through inhibition of extracellular signal-regulated kinase 1 and 2. Tissue microarray analyses of SOS1 expression in prostate carcinomas correlated with Gleason's grades of tumors, consistent with a possible role in prostate cancer progression. Investigation of prostate cancer-derived epithelial cells has led to identification of SOS1 as a potential candidate biomarker and molecular therapeutic target in prostate cancer in AA men, consistent with the hypothesis that a biological basis exists for prostate cancer aggressiveness in AA men.

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October 2009
Volume 35 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Timofeeva OA, Zhang X, Ressom HW, Varghese RS, Kallakury BV, Wang K, Ji Y, Cheema A, Jung M, Brown ML, Brown ML, et al: Enhanced expression of SOS1 is detected in prostate cancer epithelial cells from African-American men. Int J Oncol 35: 751-760, 2009
APA
Timofeeva, O.A., Zhang, X., Ressom, H.W., Varghese, R.S., Kallakury, B.V., Wang, K. ... Dritschilo, A. (2009). Enhanced expression of SOS1 is detected in prostate cancer epithelial cells from African-American men. International Journal of Oncology, 35, 751-760. https://doi.org/10.3892/ijo_00000388
MLA
Timofeeva, O. A., Zhang, X., Ressom, H. W., Varghese, R. S., Kallakury, B. V., Wang, K., Ji, Y., Cheema, A., Jung, M., Brown, M. L., Rhim, J. S., Dritschilo, A."Enhanced expression of SOS1 is detected in prostate cancer epithelial cells from African-American men". International Journal of Oncology 35.4 (2009): 751-760.
Chicago
Timofeeva, O. A., Zhang, X., Ressom, H. W., Varghese, R. S., Kallakury, B. V., Wang, K., Ji, Y., Cheema, A., Jung, M., Brown, M. L., Rhim, J. S., Dritschilo, A."Enhanced expression of SOS1 is detected in prostate cancer epithelial cells from African-American men". International Journal of Oncology 35, no. 4 (2009): 751-760. https://doi.org/10.3892/ijo_00000388